Siglec-1 (CD169) Mouse anti-Human, Brilliant Violet 605, Clone: 7-239, BD Optibuild™

Description

• The 7-239 monoclonal antibody specifically binds to Sialic acid-binding Ig-like lectin 1 (Siglec-1), which is also known as Sialoadhesin (SN), or CD169
• Siglec-1 is a type I transmembrane glycoprotein that belongs to the Siglec family within the Ig superfamily
• This adhesion molecule especiallybinds to glycolipids and glycoproteins with terminal α-2 sialyl residues.Siglec-1 is expressed by macrophages and dendritic cells and serves as a cellular interaction molecule
• Its expression can be upregulated by cells in response to type II collagen, or to cytokines including interferons, and tumor necrosis factor
• Siglec-1 plays roles in endocytosis, hematopoiesis, and leucocyte migration
• It mediates macrophage binding to various cell types including developing and mature leucocytes
• Siglec-1 that is expressed by dendritic cells can also bind HIV-1 and may mediate viral transfer to bystander CD4+ T cells
• Several Siglec-1 counter-receptors have been described including CD43, CD206, and CD227 which are expressed by T cells, macrophages, or breast cancer cells, respectively
• The 7-239 antibody reportedly blocks Siglec-1 functions in some cellular assay systems
• This antibody is conjugated to BD Horizon™ BV605 which is part of the BD Horizon Brilliant™ Violet family of dyes
• With an Ex Max of 407nm and Em Max of 602nm, BD Horizon BV605 can be excited by a violet laser and detected with a standard 610/20nm filter set
• BD Horizon BV605 is a tandem fluorochrome of BD Horizon BV421 and an acceptor dye with an Em max at 605nm
• Due to the excitation of the acceptor dye by the green (532 nm) and yellow-green (561 nm) lasers, there will be significant spillover into the PE and BD Horizon PE-CF594 detectors off the green or yellow-green lasers
• BD Horizon BV605 conjugates are very bright, often exhibiting brightness equivalent to PE conjugates and can be used as a third color off of the violet laser
• BV605 is a tandem fluorochrome that combines BD Horizon BV421 and Cy™3.5
• These conjugates are very bright, exhibiting similar brightness to equivalent PE conjugates
• Due to the excitation of Cy3.5 by the green (532nm) and yellow-green (561nm) lasers, there will be significant spillover of the BD Horizon BV605 signal into the PE and BD Horizon PE-CF594 detectors off the green or yellow-green lasers.