BDB743053

Granulocytes Mouse Anti-Rat, Brilliant Violet 421, Clone: RP-1, BD Optibuild™

Manufacturer: BD Biosciences

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Pack Size SKU Availability Price
Each of 1 BDB743053-Each-of-1 In Stock ₹ 34,224.00

BDB743053 - Each of 1

₹ 34,224.00

In Stock

Quantity

1

Base Price: ₹ 34,224.00

GST (18%): ₹ 6,160.32

Total Price: ₹ 40,384.32

Antigen

Granulocytes

Clone

RP-1

Conjugate

Brilliant Violet 421

Host Species

Mouse

Purification Method

Affinity Purified

Regulatory Status

RUO

Target Species

Mouse

Isotype

IgG2a κ

Classification

Monoclonal

Concentration

0.2mg/mL

Formulation

Aqueous buffered solution containing ≤0.09% sodium azide.

Immunogen

WKA/Hok rat peritoneal neutrophils

Quantity

50 μg

Primary or Secondary

Primary

Content And Storage

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

Description

  • The RP-1 antibody reacts with rat peritoneal and peripheral blood neutrophils and bone marrow cells
  • It does not bind rat macrophages, rat eosinophils, or peritoneal neutrophils from mice, rabbits, guinea pigs, or human peripheral blood as determined by antibody adsorption experiments
  • Flow cytometric analysis of peripheral blood leukocytes indicates that all granulocytes (cells having high side scatter), but not lymphocytes or monocytes (low side-scatter cells), express that antigen recognized by RP-1 mAb
  • Expression of the RP-1 antigen on rat peritoneal neutrophils is enhanced by stimulation with PMA or ConA
  • Immunoprecipitation of rat PMA-activated and non-treated neutrophil membranes with the RP-1 mAb produced two bands of approximately 85 kDa in m.w
  • However, the RP-1 antigen has not been biochemically characterized
  • The antibody was conjugated to BD Horizon™ BV421 which is part of the BD Horizon Brilliant™ Violet family of dyes
  • With an Ex Max of 407nm and Em Max at 421nm, BD Horizon BV421 can be excited by the violet laser and detected in the standard Pacific Blue™ filter set (eg, 450/50nm filter)
  • BD Horizon BV421 conjugates are very bright, often exhibiting a 10 fold improvement in brightness compared to Pacific Blue conjugates
  • BV421 is a polymer-based dye excited by the violet laser and is one of the brightest fluorochromes offered by BD Biosciences
  • Conjugates are typically 10 times brighter than Pacific Blue™ conjugates and are often as bright as or brighter than PE conjugates
  • Due to nearly identical excitation and emission properties but different spillover characteristics, BD Horizon BV421, Pacific Blue™, and BD Horizon™ V450 cannot be used simultaneously.

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Purification Method:
Affinity Purified

Regulatory Status:
RUO

Target Species:
Mouse

Isotype:
IgG2a κ

Classification:
Monoclonal

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0.2mg/mL

Formulation:
Aqueous buffered solution containing ≤0.09% sodium azide.

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WKA/Hok rat peritoneal neutrophils

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50 μg

Primary or Secondary:
Primary

Content And Storage:
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

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RUO

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IgG2a κ

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0.2mg/mL

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Aqueous buffered solution containing ≤0.09% sodium azide.

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Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

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Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.