BDB743057

Granulocytes Mouse Anti-Rat, Brilliant Violet 711, Clone: RP-1, BD Optibuild™

Manufacturer: BD Biosciences

Select a Size

Pack Size SKU Availability Price
Each of 1 BDB743057-Each-of-1 In Stock ₹ 34,052.88

BDB743057 - Each of 1

₹ 34,052.88

In Stock

Quantity

1

Base Price: ₹ 34,052.88

GST (18%): ₹ 6,129.518

Total Price: ₹ 40,182.398

Antigen

Granulocytes

Clone

RP-1

Conjugate

Brilliant Violet 711

Host Species

Mouse

Purification Method

Affinity Purified

Regulatory Status

RUO

Target Species

Mouse

Isotype

IgG2a κ

Classification

Monoclonal

Concentration

0.2mg/mL

Formulation

Aqueous buffered solution containing ≤0.09% sodium azide.

Immunogen

WKA/Hok rat peritoneal neutrophils

Quantity

50 μg

Primary or Secondary

Primary

Content And Storage

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

Description

  • The RP-1 antibody reacts with rat peritoneal and peripheral blood neutrophils and bone marrow cells
  • It does not bind rat macrophages, rat eosinophils, or peritoneal neutrophils from mice, rabbits, guinea pigs, or human peripheral blood as determined by antibody adsorption experiments
  • Flow cytometric analysis of peripheral blood leukocytes indicates that all granulocytes (cells having high side scatter), but not lymphocytes or monocytes (low side-scatter cells), express that antigen recognized by RP-1 mAb
  • Expression of the RP-1 antigen on rat peritoneal neutrophils is enhanced by stimulation with PMA or ConA
  • Immunoprecipitation of rat PMA-activated and non-treated neutrophil membranes with the RP-1 mAb produced two bands of approximately 85 kDa in m.w
  • However, the RP-1 antigen has not been biochemically characterized
  • The antibody was conjugated to BD Horizon™ BV711 which is part of the BD Horizon Brilliant™ Violet family of dyes
  • This dye is a tandem fluorochrome of BD Horizon BV421 with an Ex Max of 405nm and an acceptor dye with an Em Max at 711nm
  • BD Horizon BV711 can be excited by the violet laser and detected in a filter used to detect Cy™5.5 / Alexa Fluor™ 700-like dyes (eg, 712/20nm filter)
  • Due to the excitation and emission characteristics of the acceptor dye, there may be moderate spillover into the Alexa Fluor™ 700 and PerCP-Cy5.5 detectors
  • However, the spillover can be corrected through compensation as with any other dye combination
  • BV711 is a tandem fluorochrome of BD Horizon BV421 and an acceptor dye with an emission maximum at 711 nm
  • This dye offers a very bright choice for the violet laser
  • Due to the excitation and emission characteristics of the acceptor dye, there may be moderate spillover into the Alexa Fluor™ 700 and PerCP-Cy5.5 detectors
  • BV711 will also have moderate spillover into the BD Horizon Brilliant™ Violet 786 detector.

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Antigen:
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Clone:
RP-1

Conjugate:
Brilliant Violet 711

Host Species:
Mouse

Purification Method:
Affinity Purified

Regulatory Status:
RUO

Target Species:
Mouse

Isotype:
IgG2a κ

Classification:
Monoclonal

Concentration:
0.2mg/mL

Formulation:
Aqueous buffered solution containing ≤0.09% sodium azide.

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WKA/Hok rat peritoneal neutrophils

Quantity:
50 μg

Primary or Secondary:
Primary

Content And Storage:
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

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Affinity Purified

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RUO

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Formulation:
Aqueous buffered solution containing ≤0.09% sodium azide.

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Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

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IgG2a κ

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0.2mg/mL

Formulation:
Aqueous buffered solution containing ≤0.09% sodium azide.

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Quantity:
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Primary or Secondary:
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Content And Storage:
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.