CD122 Mouse anti-Human, Brilliant Violet 421, Clone: Mik-β2, BD Optibuild™

Description

• The Mik-β2monoclonal antibody specifically recognizes the 75 kDβsubunit (p75) of the IL-2 receptor (IL-2Rβ)
• Cell surface IL-2Rβmolecules are expressed by T cells, B cells, monocytes, myeloid precursors, NK cells, and LGL
• Together with theαsubunit (p55, CD25) and the commonγsubunit (CD132;γc subunit of the IL-2R, IL-4R, and IL-7R), the IL-2Rβmolecule forms a high-affinity, signaling receptor complex for IL-2 which can be expressed by activated T and B lymphocytes
• Alternatively, some cell types, such as NK cells and myeloid cell populations, coexpress IL-2Rβmolecules andγc subunits to form intermediate-affinity, signalling receptor complexes for IL-2
• The antibody was conjugated to BD Horizon™ BV421 which is part of the BD Horizon Brilliant™ Violet family of dyes
• With an Ex Max of 407nm and Em Max at 421nm, BD Horizon BV421 can be excited by the violet laser and detected in the standard Pacific Blue™ filter set (eg, 450/50nm filter)
• BD Horizon BV421 conjugates are very bright, often exhibiting a 10 fold improvement in brightness compared to Pacific Blue conjugates
• BV421 is a polymer-based dye excited by the violet laser and is one of the brightest fluorochromes offered by BD Biosciences
• Conjugates are typically 10 times brighter than Pacific Blue™ conjugates and are often as bright as or brighter than PE conjugates
• Due to nearly identical excitation and emission properties but different spillover characteristics, BD Horizon BV421, Pacific Blue™, and BD Horizon™ V450 cannot be used simultaneously.