CD45R Mouse Anti-Rat, Brilliant Violet 711, Clone: HIS24, BD Optibuild™

Description

• The HIS24 antibody reacts with a developmentally regulated form of CD45 found on most B lymphocytes, including developing B cells in the bone marrow and peripheral B cells, but not plasma cells
• The level of expression of this CD45R antigen appears to be an indicator of both maturational stages in the B-cell lineage and of functionally distinct B-cell subsets
• The HIS24 mAb, in combination with other markers such as TdT and Ig expression, is effective in the identification of B-cell progenitors
• CD45 is a member of the Protein Tyrosine Phosphatase (PTP) family: Its intracellular (COOH-terminal) region contains two PTP catalytic domains, and the extracellular region is highly variable due to alternative splicing of exons 4, 5, and 6 (designated A, B, and C, respectively), plus differing levels of glycosylation
• The CD45 isoforms detected in the rat are cell type-, maturation-, and activation state-specific
• The CD45 isoforms play complex roles in T-cell and B-cell antigen receptor signal transduction
• The antibody was conjugated to BD Horizon™ BV711 which is part of the BD Horizon Brilliant™ Violet family of dyes
• This dye is a tandem fluorochrome of BD Horizon BV421 with an Ex Max of 405nm and an acceptor dye with an Em Max at 711nm
• BD Horizon BV711 can be excited by the violet laser and detected in a filter used to detect Cy™5.5 / Alexa Fluor™ 700-like dyes (eg, 712/20nm filter)
• Due to the excitation and emission characteristics of the acceptor dye, there may be moderate spillover into the Alexa Fluor™ 700 and PerCP-Cy5.5 detectors
• However, the spillover can be corrected through compensation as with any other dye combination
• BV711 is a tandem fluorochrome of BD Horizon BV421 and an acceptor dye with an emission maximum at 711 nm
• This dye offers a very bright choice for the violet laser
• Due to the excitation and emission characteristics of the acceptor dye, there may be moderate spillover into the Alexa Fluor™ 700 and PerCP-Cy5.5 detectors
• BV711 will also have moderate spillover into the BD Horizon Brilliant™ Violet 786 detector.