CD42d Mouse Anti-Rat, Brilliant Violet 650, Clone: RPM.4, BD Optibuild™

Description

• The RPM.4 monoclonal antibody specifically recognizes CD42d (Platelet glycoprotein V, GPV)
• CD42d is a 88-kDa type I transmembrane glycoprotein which noncovalently associates with GPIb and GPIX to form a receptor for von Willebrand factor on megakaryocytes and resting platelets
• The binding sites for von Willebrand factor and thrombin have been localized to the GPIb chain of the GPI-b-V-IX complex
• GPV is cleaved by thrombin to release a 70 kDa soluble fragment, which contains the epitope recognized by mAb RPM.4
• Although the rat CD42d protein's structure is similar to that of human CD42d, the RPM.4 antibody does not cross-react with human platelets
• The antibody was conjugated to BD Horizon™ BV650 which is part of the BD Horizon Brilliant™ Violet family of dyes
• This dye is a tandem fluorochrome of BD Horizon BV421 with an Ex Max of 405nm and an acceptor dye with an Em Max at 650nm
• BD Horizon BV650 can be excited by the violet laser and detected in a filter used to detect APC-like dyes (eg, 660/20nm filter)
• Due to the excitation and emission characteristics of the acceptor dye, there will be spillover into the APC and Alexa Fluor™ 700 detectors
• However, the spillover can be corrected through compensation as with any other dye combination
• BV650 is a tandem fluorochrome of BD Horizon BV421 and an acceptor dye with an emission maximum at 650 nm
• Due to the excitation and emission characteristics of the acceptor dye, there will be spillover into the APC and Alexa Fluor™ 700 detectors
• BV650 will have moderate spillover into the BD Horizon™ BV711 detector.