CD43 Mouse anti-Human, Brilliant Violet 711, Clone: 1G10, BD Optibuild™

Description

• The 1G10 monoclonal antibody specifically binds to CD43, a 95-135 kDa sialoglycoprotein that is highly expressed on most human leucocytes
• CD43 is encoded by the SPN /gene and is also known as Sialophorin, Galactoglycoprotein (GALGP), Leukosialin, and Leukocyte sialoglycoprotein
• The CD43 antigen is expressed on T cells, pre-B cells and activated B cells, NK cells and granulocytes, but is not present on resting peripheral blood B cells, red blood cells, platelets and non-hematopoietic cells
• CD43 is enzymatically shed from leucocyte surfaces following activation by various stimuli
• CD43 appears to be involved in intercellular interactions that regulate T, B, and NK cell functions
• This antibody is suitable for staining formalin-fixed, paraffin-embedded tissue sections with TUF pretreatment
• The antibody was conjugated to BD Horizon™ BV711 which is part of the BD Horizon Brilliant™ Violet family of dyes
• This dye is a tandem fluorochrome of BD Horizon BV421 with an Ex Max of 405nm and an acceptor dye with an Em Max at 711nm
• BD Horizon BV711 can be excited by the violet laser and detected in a filter used to detect Cy™5.5 / Alexa Fluor™ 700-like dyes (eg, 712/20nm filter)
• Due to the excitation and emission characteristics of the acceptor dye, there may be moderate spillover into the Alexa Fluor™ 700 and PerCP-Cy5.5 detectors
• However, the spillover can be corrected through compensation as with any other dye combination
• BV711 is a tandem fluorochrome of BD Horizon BV421 and an acceptor dye with an emission maximum at 711 nm
• This dye offers a very bright choice for the violet laser
• Due to the excitation and emission characteristics of the acceptor dye, there may be moderate spillover into the Alexa Fluor™ 700 and PerCP-Cy5.5 detectors
• BV711 will also have moderate spillover into the BD Horizon Brilliant™ Violet 786 detector.