CD155 Rat anti-Mouse, Brilliant Violet 605, Clone: 3F1, BD Optibuild™

Description

• The 3F1 monoclonal antibody specifically binds to CD155, which is also known as Poliovirus receptor (Pvr) or Tumor-associated antigen 1 (Taa1)
• CD155 is a type I transmembrane glycoprotein that belongs to the Ig supergene family
• CD155 is an adhesion receptor that binds to different ligands including nectin-3, CD96, CD226, TIGIT, and the extracellular matrix protein vitronectin
• It is highly expressed on double positive thymocytes and variably expressed on mature thymocytes and T cells, including regulatory T cells and NKT cells
• CD155 is also differentially expressed on subsets of B cells, plasma cells, dendritic cells, and monocytes
• CD155 expression is upregulated by activated T cells, B cells, and dendritic cells
• CD155 is involved in forming adherens junctions between adjacent epithelial or endothelial cells
• CD155 plays roles in regulating cell growth, adhesion, motility, migration, and cell-mediated cytotoxicity
• CD155-deficient mice exhibit impaired secondary humoral immune responses to orally administered antigens
• This antibody is conjugated to BD Horizon™ BV605 which is part of the BD Horizon Brilliant™ Violet family of dyes
• With an Ex Max of 407nm and Em Max of 602nm, BD Horizon BV605 can be excited by a violet laser and detected with a standard 610/20nm filter set
• BD Horizon BV605 is a tandem fluorochrome of BD Horizon BV421 and an acceptor dye with an Em max at 605nm
• Due to the excitation of the acceptor dye by the green (532 nm) and yellow-green (561 nm) lasers, there will be significant spillover into the PE and BD Horizon PE-CF594 detectors off the green or yellow-green lasers
• BD Horizon BV605 conjugates are very bright, often exhibiting brightness equivalent to PE conjugates and can be used as a third color off of the violet laser
• BV605 is a tandem fluorochrome that combines BD Horizon BV421 and Cy™3.5
• These conjugates are very bright, exhibiting similar brightness to equivalent PE conjugates
• Due to the excitation of Cy3.5 by the green (532nm) and yellow-green (561nm) lasers, there will be significant spillover of the BD Horizon BV605 signal into the PE and BD Horizon PE-CF594 detectors off the green or yellow-green lasers.