CD1d Mouse anti-Human, Brilliant Violet 650, Clone: CD1d42 (also known as 42.1), BD Optibuild™

Description

• The CD1d42 monoclonal antibody recognizes CD1d
• Cell surface CD1d is structurally homologous to Class I MHC molecules
• It consists ofa glycosylated type I transmembrane α chain (43-49 kDa) that is non-covalently associated with β2-microglobulin
• CD1d is a member of the CD1 family of molecules, which belong to the immunoglobulin superfamily
• Sequence homology data classifies the CD1 molecules into two groups
• Group 1 includes CD1a, CD1b andCD1c molecules; group 2 includes CD1d molecules and their homologs in other species
• CD1d is expressed on cortical thymocytes, B cells, dendritic cells, monocytes, and some nonlymphoid cells including intestinal epithelial cells, hepatocytes and keratinocytes
• It is not expressed on resting mature T cells
• Studies suggest that CD1d participates in lipid antigen presentation to CD1d-restricted NKT cells The antibody was conjugated to BD Horizon™ BV650 which is part of the BD Horizon Brilliant™ Violet family of dyes
• This dye is a tandem fluorochrome of BD Horizon BV421 with an Ex Max of 405nm and an acceptor dye with an Em Max at 650nm
• BD Horizon BV650 can be excited by the violet laser and detected in a filter used to detect APC-like dyes (eg, 660/20nm filter)
• Due to the excitation and emission characteristics of the acceptor dye, there will be spillover into the APC and Alexa Fluor™ 700 detectors
• However, the spillover can be corrected through compensation as with any other dye combination
• BV650 is a tandem fluorochrome of BD Horizon BV421 and an acceptor dye with an emission maximum at 650 nm
• Due to the excitation and emission characteristics of the acceptor dye, there will be spillover into the APC and Alexa Fluor™ 700 detectors
• BV650 will have moderate spillover into the BD Horizon™ BV711 detector.