CD266 Mouse anti-Human, Brilliant Violet 650, Clone: ITEM-1, BD Optibuild™

Description

• The ITEM-1 monoclonal antibody specifically binds to CD266, which is also known as TWEAK Receptor (TWEAK-R/TWEAKR), or Fibroblast growth factor inducible 14 (FGF-inducible 14, FN14)
• CD266 is a ñ14 kDa type I transmembrane protein that is the 12A family member of the tumor necrosis factor receptor superfamily (TNFRSF12A)
• It is expressed at low levels in a variety of tissues including, heart, placenta, lung, muscle, and pancreas
• CD266 is relatively highly expressed on HUVEC cells and certain tumor cell lines
• TWEAK-induced CD266-mediated signaling can play roles in inflammation, the induction of apoptosis in certain cell types, the proliferation and migration of endothelial cells, and can promote angiogenesis within healthy and diseased tissues, eg, tumors
• The ITEM-1 antibody can reportedly induce the proliferation of HUVEC cells and the death of certain TWEAK-sensitive tumor target cell lines
• CD266 binds TWEAK (CD255) and can signal intracellularly by interactions with TRAF1, 2, and 3 cytoplasmic proteins leading to NF-κB activation
• The antibody was conjugated to BD Horizon™ BV650 which is part of the BD Horizon Brilliant™ Violet family of dyes
• This dye is a tandem fluorochrome of BD Horizon BV421 with an Ex Max of 405nm and an acceptor dye with an Em Max at 650nm
• BD Horizon BV650 can be excited by the violet laser and detected in a filter used to detect APC-like dyes (eg, 660/20nm filter)
• Due to the excitation and emission characteristics of the acceptor dye, there will be spillover into the APC and Alexa Fluor™ 700 detectors
• However, the spillover can be corrected through compensation as with any other dye combination
• BV650 is a tandem fluorochrome of BD Horizon BV421 and an acceptor dye with an emission maximum at 650 nm
• Due to the excitation and emission characteristics of the acceptor dye, there will be spillover into the APC and Alexa Fluor™ 700 detectors
• BV650 will have moderate spillover into the BD Horizon™ BV711 detector.