CD116 Mouse anti-Human, Brilliant Violet 510, Clone: hGMCSFR-M1, BD Optibuild™

Description

• The hGMCSFR-M1 antibody reacts with the subunit (GM-CSFR) of the human Granulocyte-Macrophage Colony-Stimulating Factor Receptor complex
• This 75-85 kD subunit is also known as CD116
• The hGMCSFR-M1 antibody was first clustered at the Fifth International Workshop on Human Leucocyte Differentiation Antigens
• The GM-CSFR subunit associates with the 120-140 kDβc subunit (common subunit, CD131), that is shared with the receptors for interleukins IL-3 and IL-5
• Both of the chains of the GM-CSFR complex are involved in ligand binding and intracellular signaling
• Theαchain appears to transmit most of the biological signals
• CD116 is expressed by a variety of myeloid cell lines, hematopoietic and non-hematopoetic tumor cells, and normal cell types including monocytes, macrophages, neutrophils, eosinophils, myeloid dendritic cells, endothelial cells, fibroblasts, and placental trophoblasts
• Lymphocytes are negative for GM-CSFR expression
• Reports suggest that GM-CSFR plays a role in myeloid lineage growth and differentiation
• The immunogen used to generate the hGMCSFR-M1 hybridoma was recombinant human GM-CSFR
• The antibody was conjugated to BD Horizon™ BV510 which is part of the BD Horizon Brilliant™ Violet family of dyes
• With an Ex Max of 405nm and Em Max at 510nm, BD Horizon BV510 can be excited by the violet laser and detected in the BD Horizon V500 (525/50nm) filter set
• BD Horizon BV510 conjugates are useful for the detection of dim markers off the violet laser
• BV510 is a polymer-based dye that is brighter than BD Horizon V500
• Due to similar excitation and emission properties, BD Horizon BV510 and BD Horizon V500 cannot be used simultaneously.