CD314 (NKG2D) Mouse anti-Human, BUV395, Clone: 1D11, BD Optibuild™

Description

• The 1D11 monoclonal antibody specifically binds to NKG2D, a 42 kDa type II transmembrane glycoprotein that is also known as CD314 and KLRK1
• NKG2D is a member of the C-type lectin family and is expressed on human NK cells
• This activating receptor binds strongly to several ligands including MICA and MICB and ULBP-1, -2, and -3 proteins that are expressed by different target cell types
• Different from natural cytotoxicity receptor (NCR), NKG2D expression is not confined to NK cells
• It is also expressed on virtually all TCR γ/δ+ and CD8+TCR α/β+ T cells
• NKG2D functions as a triggering receptor involved in natural cytotoxicity mediated by normal NK cells against a variety of tumors or normal target cells
• Importantly, NKG2D can complement the role of NCR in tumor cell lysis
• Remarkably, the combined maskings of NCR and NKG2D can reportedly lead to a complete inhibition of NK-mediated lysis of all tumor or normal cells
• The 1D11 antibody can reportedly block or stimulate the function of NKG2D-positive cells The antibody was conjugated to BD Horizon™ BUV395 which is part of the BD Horizon Brilliant™ Ultraviolet family of dyes
• This dye has been exclusively developed by BD Biosciences to have minimal spillover into other detectors, making it an optimal choice for multicolor flow cytometry
• With an Ex Max at 348 nm and an Em Max at 395 nm, BD Horizon BUV395 can be excited with a 355 nm laser and detected with a 379/28 filter
• BUV395 is a UV-excitable dye that has been developed exclusively by BD Biosciences
• With an excitation max of 348 nm and emission max of 395 nm, BUV395 can be excited by the 355nm laser and detected in a 379/28 filter
• This dye is optimal for multicolor flow cytometry because it has little to no spillover into other detectors.