CD172a/b Mouse anti-Human, Brilliant Violet 510, Clone: SE5A5, BD Optibuild™

Description

• The SE5A5 monoclonal antibody specifically binds to a common epitope on CD172a/ SIRPα (90 kDa) and CD172b/ SIRPβ 1 (50 kDa)
• These transmembrane glycoproteins are members of the Signal Regulatory Protein (SIRP) family that, in turn, belongs to the Immunoglobulin superfamily
• The SIRP family is comprised of two subgroups, SIRPα and SIRPβ that are distinguished by the presence (α) or absence (β) of a cytoplasmic domain containing two immunoreceptor tyrosine-based inhibition motifs (ITIM)
• CD172a/ SIRPα is expressed on CD34+ stem/progenitor cells, cardiomyocytes, monocytes, macrophages, granulocytes, dendritic cells, and in the central nervous system
• It binds to CD47 and is implicated in mediating inhibitory signals via the ITIM/SHP-2 association
• CD172b/ SIRPβ 1 does not possess a cytoplasmic domain but instead the transmembrane domain contains a positively-charged residue that can interact with another transmembrane protein, DAP-12/KARAP
• DAP-12 has two immunoreceptor tyrosine-based activat ion motifs (ITAM) within its cytoplasmic domain that are thought to link CD172b to cellular activation signaling
• CD172b is expressed on myeloid cells, including peripheral blood monocytes and granulocytes
• It is not expressed on CD34+ cells
• CD172a and CD172b have complementary role s in signal regulation and may work together in tuning certain cellular responses to stimuli
• The antibody was conjugated to BD Horizon™ BV510 which is part of the BD Horizon Brilliant™ Violet family of dyes
• With an Ex Max of 405nm and Em Max at 510nm, BD Horizon BV510 can be excited by the violet laser and detected in the BD Horizon V500 (525/50nm) filter set
• BD Horizon BV510 conjugates are useful for the detection of dim markers off the violet laser
• BV510 is a polymer-based dye that is brighter than BD Horizon V500
• Due to similar excitation and emission properties, BD Horizon BV510 and BD Horizon V500 cannot be used simultaneously.