CD324 (E-Cadherin) Mouse anti-Human, Brilliant Violet 421, Clone: 67A4, BD Optibuild™

Description

• The 67A4 monoclonal antibody specifically recognizes the extracellular domain of human E-Cadherin (CD324)
• E-Cadherin is a 120-kDa transmembrane glycoprotein that is localized in the adherens junctions of epithelial cells
• There it interacts with the cytoskeleton through the associated cytoplasmic catenin proteins
• In addition to being a calcium-dependent adhesion molecule, E-Cadherin is also a critical regulator of epithelial junction formation
• Its association with catenins is necessary for cell-to-cell adhesion
• These E-Cadherin/catenin complexes associate with cortical actin bundles at both the zonula adherens and the lateral adhesion plaques
• Tyrosine phosphorylation can disrupt these complexes, leading to changes in cell adhesion properties
• E-Cadherin expression is often down-regulated in highly invasive, poorly differentiated carcinomas
• Increased expression of E-Cadherin in these cells reduces their invasiveness
• Thus, loss of expression or function of E-Cadherin appears to be an important step in tumorigenic progression
• Pluripotent stem cells express E-Cadherin
• Upon differentiation, an epithelial to mesenchymal transition results in the loss of E-cadherin expression and a gain in the expression of N-cadherin
• The antibody was conjugated to BD Horizon™ BV421 which is part of the BD Horizon Brilliant™ Violet family of dyes
• With an Ex Max of 407nm and Em Max at 421nm, BD Horizon BV421 can be excited by the violet laser and detected in the standard Pacific Blue™ filter set (eg, 450/50nm filter)
• BD Horizon BV421 conjugates are very bright, often exhibiting a 10 fold improvement in brightness compared to Pacific Blue conjugates
• BV421 is a polymer-based dye excited by the violet laser and is one of the brightest fluorochromes offered by BD Biosciences
• Conjugates are typically 10 times brighter than Pacific Blue™ conjugates and are often as bright as or brighter than PE conjugates
• Due to nearly identical excitation and emission properties but different spillover characteristics, BD Horizon BV421, Pacific Blue™, and BD Horizon™ V450 cannot be used simultaneously.