CD42a Mouse anti-Human, Brilliant Violet 605, Clone: ALMA.16, BD Optibuild™

Description

• The ALMA.16 monoclonal antibody specifically recognizes CD42a
• CD42a is a 17-22 kDa type I transmembrane glycoprotein that is also known as Platelet glycoprotein IX (GPIX), or Glycoprotein 9 (GP9)
• CD42a forms a noncovalently linked complex (GPIb/GPIX/GPV) with CD42b, CD42c and CD42d that may serve as a receptor for von Willebrand factor
• It is expressed on platelets and megakaryocytes and is absent on the platelets of patients with Bernadr-Soulier Syndrome (BSS)
• Although the CD42a function is not fully understood, GPIX glycoprotein is important for the assembly and membrane expression of the CD42 complex and for the maintenance of the functional conformation of CD42b (GPIb)
• This antibody is conjugated to BD Horizon™ BV605 which is part of the BD Horizon Brilliant™ Violet family of dyes
• With an Ex Max of 407nm and Em Max of 602nm, BD Horizon BV605 can be excited by a violet laser and detected with a standard 610/20nm filter set
• BD Horizon BV605 is a tandem fluorochrome of BD Horizon BV421 and an acceptor dye with an Em max at 605nm
• Due to the excitation of the acceptor dye by the green (532 nm) and yellow-green (561 nm) lasers, there will be significant spillover into the PE and BD Horizon PE-CF594 detectors off the green or yellow-green lasers
• BD Horizon BV605 conjugates are very bright, often exhibiting brightness equivalent to PE conjugates and can be used as a third color off of the violet laser
• BV605 is a tandem fluorochrome that combines BD Horizon BV421 and Cy™3.5
• These conjugates are very bright, exhibiting similar brightness to equivalent PE conjugates
• Due to the excitation of Cy3.5 by the green (532nm) and yellow-green (561nm) lasers, there will be significant spillover of the BD Horizon BV605 signal into the PE and BD Horizon PE-CF594 detectors off the green or yellow-green lasers.