CD46 Mouse anti-Human, Brilliant Violet 421, Clone: E4.3, BD Optibuild™

Description

• The E4.3 monoclonal antibody specifically binds to CD46
• CD46 is also known as membrane cofactor protein (MCP)
• CD46 is a type I membrane glycoprotein composed of two non-disulfide linkedα(66 kDa) andβ(56 kDa) chains expressed on lymphocytes, monocytes and granulocytes
• It is not expressed on erythrocytes or platelets
• There are four isoforms of the dimer which function as complement regulatory factors
• CD46 promotes the enzymatic degradation of activated C3b and/or C4b deposited on host cells
• It also serves as the measles virus receptor
• The antibody was conjugated to BD Horizon™ BV421 which is part of the BD Horizon Brilliant™ Violet family of dyes
• With an Ex Max of 407nm and Em Max at 421nm, BD Horizon BV421 can be excited by the violet laser and detected in the standard Pacific Blue™ filter set (eg, 450/50nm filter)
• BD Horizon BV421 conjugates are very bright, often exhibiting a 10 fold improvement in brightness compared to Pacific Blue conjugates
• BV421 is a polymer-based dye excited by the violet laser and is one of the brightest fluorochromes offered by BD Biosciences
• Conjugates are typically 10 times brighter than Pacific Blue™ conjugates and are often as bright as or brighter than PE conjugates
• Due to nearly identical excitation and emission properties but different spillover characteristics, BD Horizon BV421, Pacific Blue™, and BD Horizon™ V450 cannot be used simultaneously.