Integrin β7 Chain Rat anti-Mouse, Brilliant Violet 605, Clone: M293, BD Optibuild™

Description

• The M293 monoclonal antibody specifically recognizes the integrin ß7 chain (also known as integrin ßp), which is found in association with either of twoαchains.α4ß7 (LPAM-1) is expressed on most mature lymphocytes and on small subsets of thymic and bone marrow cells; it interacts with several ligands, including VCAM-1, fibronectin, and MAdCAM-1.αIELß7 is found primarily on intestinal mucosal and intraepithelial lymphocytes (IEL), and it may be primarily involved in interactions between IEL and epithelia via /recognition of E-cadherin
• The epitope recognized by the mAb M293 has been mapped to the region between amino acids 387 and 542
• The M293 antibody exhibits little or no activity in blocking ß7 integrin-mediated adhesion
• This antibody is conjugated to BD Horizon™ BV605 which is part of the BD Horizon Brilliant™ Violet family of dyes
• With an Ex Max of 407nm and Em Max of 602nm, BD Horizon BV605 can be excited by a violet laser and detected with a standard 610/20nm filter set
• BD Horizon BV605 is a tandem fluorochrome of BD Horizon BV421 and an acceptor dye with an Em max at 605nm
• Due to the excitation of the acceptor dye by the green (532 nm) and yellow-green (561 nm) lasers, there will be significant spillover into the PE and BD Horizon PE-CF594 detectors off the green or yellow-green lasers
• BD Horizon BV605 conjugates are very bright, often exhibiting brightness equivalent to PE conjugates and can be used as a third color off of the violet laser
• BV605 is a tandem fluorochrome that combines BD Horizon BV421 and Cy™3.5
• These conjugates are very bright, exhibiting similar brightness to equivalent PE conjugates
• Due to the excitation of Cy3.5 by the green (532nm) and yellow-green (561nm) lasers, there will be significant spillover of the BD Horizon BV605 signal into the PE and BD Horizon PE-CF594 detectors off the green or yellow-green lasers.