Integrin β7 Chain Rat anti-Mouse, Brilliant Violet 711, Clone: M293, BD Optibuild™

Description

• The M293 monoclonal antibody specifically recognizes the integrin ß7 chain (also known as integrin ßp), which is found in association with either of twoαchains.α4ß7 (LPAM-1) is expressed on most mature lymphocytes and on small subsets of thymic and bone marrow cells; it interacts with several ligands, including VCAM-1, fibronectin, and MAdCAM-1.αIELß7 is found primarily on intestinal mucosal and intraepithelial lymphocytes (IEL), and it may be primarily involved in interactions between IEL and epithelia via /recognition of E-cadherin
• The epitope recognized by the mAb M293 has been mapped to the region between amino acids 387 and 542
• The M293 antibody exhibits little or no activity in blocking ß7 integrin-mediated adhesion
• The antibody was conjugated to BD Horizon™ BV711 which is part of the BD Horizon Brilliant™ Violet family of dyes
• This dye is a tandem fluorochrome of BD Horizon BV421 with an Ex Max of 405nm and an acceptor dye with an Em Max at 711nm
• BD Horizon BV711 can be excited by the violet laser and detected in a filter used to detect Cy™5.5 / Alexa Fluor™ 700-like dyes (eg, 712/20nm filter)
• Due to the excitation and emission characteristics of the acceptor dye, there may be moderate spillover into the Alexa Fluor™ 700 and PerCP-Cy5.5 detectors
• However, the spillover can be corrected through compensation as with any other dye combination
• BV711 is a tandem fluorochrome of BD Horizon BV421 and an acceptor dye with an emission maximum at 711 nm
• This dye offers a very bright choice for the violet laser
• Due to the excitation and emission characteristics of the acceptor dye, there may be moderate spillover into the Alexa Fluor™ 700 and PerCP-Cy5.5 detectors
• BV711 will also have moderate spillover into the BD Horizon Brilliant™ Violet 786 detector.