Integrin β7 Chain Rat anti-Mouse, BUV395, Clone: M293, BD Optibuild™

Description

• The M293 monoclonal antibody specifically recognizes the integrin ß7 chain (also known as integrin ßp), which is found in association with either of twoαchains.α4ß7 (LPAM-1) is expressed on most mature lymphocytes and on small subsets of thymic and bone marrow cells; it interacts with several ligands, including VCAM-1, fibronectin, and MAdCAM-1.αIELß7 is found primarily on intestinal mucosal and intraepithelial lymphocytes (IEL), and it may be primarily involved in interactions between IEL and epithelia via /recognition of E-cadherin
• The epitope recognized by the mAb M293 has been mapped to the region between amino acids 387 and 542
• The M293 antibody exhibits little or no activity in blocking ß7 integrin-mediated adhesion
• The antibody was conjugated to BD Horizon™ BUV395 which is part of the BD Horizon Brilliant™ Ultraviolet family of dyes
• This dye has been exclusively developed by BD Biosciences to have minimal spillover into other detectors, making it an optimal choice for multicolor flow cytometry
• With an Ex Max at 348 nm and an Em Max at 395 nm, BD Horizon BUV395 can be excited with a 355 nm laser and detected with a 379/28 filter
• BUV395 is a UV-excitable dye that has been developed exclusively by BD Biosciences
• With an excitation max of 348 nm and emission max of 395 nm, BUV395 can be excited by the 355nm laser and detected in a 379/28 filter
• This dye is optimal for multicolor flow cytometry because it has little to no spillover into other detectors.