IgM[a] Mouse anti-Mouse, Brilliant Violet 650, Clone: DS-1, BD Optibuild™

Description

• The DS-1 antibody reacts specifically with mouse IgM of Igh-C[a] /and related haplotypes (e.g., BALB/c, C58, CBA, C3H/Bi, C3H/He, DBA/1, DBA/2)
• It does not react with IgM of Igh-C[b], Igh-C[e] /, or related hapltypes (e.g., C57BL, SJL, A, AKR, NZB)
• DS-1 antibody does not react with free μ heavy chain in vitro or in the cytoplasm of pre-B lymphocytes, which lack Ig light chain
• It has not been shown to stimulate B-cell proliferation
• The antibody was conjugated to BD Horizon™ BV650 which is part of the BD Horizon Brilliant™ Violet family of dyes
• This dye is a tandem fluorochrome of BD Horizon BV421 with an Ex Max of 405nm and an acceptor dye with an Em Max at 650nm
• BD Horizon BV650 can be excited by the violet laser and detected in a filter used to detect APC-like dyes (eg, 660/20nm filter)
• Due to the excitation and emission characteristics of the acceptor dye, there will be spillover into the APC and Alexa Fluor™ 700 detectors
• However, the spillover can be corrected through compensation as with any other dye combination
• BV650 is a tandem fluorochrome of BD Horizon BV421 and an acceptor dye with an emission maximum at 650 nm
• Due to the excitation and emission characteristics of the acceptor dye, there will be spillover into the APC and Alexa Fluor™ 700 detectors
• BV650 will have moderate spillover into the BD Horizon™ BV711 detector.