IgM[a] Mouse anti-Mouse, Brilliant Violet 711, Clone: DS-1, BD Optibuild™

Description

• The DS-1 antibody reacts specifically with mouse IgM of Igh-C[a] /and related haplotypes (e.g., BALB/c, C58, CBA, C3H/Bi, C3H/He, DBA/1, DBA/2)
• It does not react with IgM of Igh-C[b], Igh-C[e] /, or related hapltypes (e.g., C57BL, SJL, A, AKR, NZB)
• DS-1 antibody does not react with free μ heavy chain in vitro or in the cytoplasm of pre-B lymphocytes, which lack Ig light chain
• It has not been shown to stimulate B-cell proliferation
• The antibody was conjugated to BD Horizon™ BV711 which is part of the BD Horizon Brilliant™ Violet family of dyes
• This dye is a tandem fluorochrome of BD Horizon BV421 with an Ex Max of 405nm and an acceptor dye with an Em Max at 711nm
• BD Horizon BV711 can be excited by the violet laser and detected in a filter used to detect Cy™5.5 / Alexa Fluor™ 700-like dyes (eg, 712/20nm filter)
• Due to the excitation and emission characteristics of the acceptor dye, there may be moderate spillover into the Alexa Fluor™ 700 and PerCP-Cy5.5 detectors
• However, the spillover can be corrected through compensation as with any other dye combination
• BV711 is a tandem fluorochrome of BD Horizon BV421 and an acceptor dye with an emission maximum at 711 nm
• This dye offers a very bright choice for the violet laser
• Due to the excitation and emission characteristics of the acceptor dye, there may be moderate spillover into the Alexa Fluor™ 700 and PerCP-Cy5.5 detectors
• BV711 will also have moderate spillover into the BD Horizon Brilliant™ Violet 786 detector.