Endosialin (CD248) Mouse anti-Human, Brilliant Violet 421, Clone: B1/35, BD Optibuild™

Description

• The B1/35 monoclonal antibody specifically binds to CD248 which is also known as Endosialin, Tumor endothelial marker 1 (TEM1), and CD164 sialomucin-like 1 (CD164L1)
• CD248 is a heavily glycosylated, single-pass type I transmembrane protein
• CD248 belongs to the Group XIV C-Type lectin family that includes CD93 and CD141/thrombomodulin
• It is expressed on pericytes and stromal fibroblasts
• Although the exact functions of CD248 remain to be determined, its expression is associated with angiogenesis and lymphoid tissue organization during development, as well as, with postnatal inflammation and tumor development and growth
• The antibody was conjugated to BD Horizon™ BV421 which is part of the BD Horizon Brilliant™ Violet family of dyes
• With an Ex Max of 407nm and Em Max at 421nm, BD Horizon BV421 can be excited by the violet laser and detected in the standard Pacific Blue™ filter set (eg, 450/50nm filter)
• BD Horizon BV421 conjugates are very bright, often exhibiting a 10 fold improvement in brightness compared to Pacific Blue conjugates
• BV421 is a polymer-based dye excited by the violet laser and is one of the brightest fluorochromes offered by BD Biosciences
• Conjugates are typically 10 times brighter than Pacific Blue™ conjugates and are often as bright as or brighter than PE conjugates
• Due to nearly identical excitation and emission properties but different spillover characteristics, BD Horizon BV421, Pacific Blue™, and BD Horizon™ V450 cannot be used simultaneously.