CD137 Ligand Mouse anti-Human, Brilliant Violet 421, Clone: C65-485, BD Optibuild™

Description

• The C65-485 monoclonal antibody specifically recognizes the type II membrane protein known as 4-1BB Ligand (4-1BB-L) or CD137 Ligand (CD137L)
• This molecule belongs to the TNF superfamily and is encoded by TNFSF9 /(Tumor necrosis factor superfamily member 9)
• CD137 Ligand has been reported to have costimulatory function on activated T cells
• Reports suggest that stimulation of activated T cells via CD137 Ligand does not require the presence of other costimulatory molecules, including CD28
• Some studies have reported human CD137 Ligand to be expressed preferentially on primary B cells and B-cell lines
• The C65-485 antibody is able to react with recombinant human 4-1BB Ligand (rh4-1BB-L/CD137L) bound to CD137 (4-1BB receptor) expressed on phytohemagglutinin (PHA)-stimulated PBMC
• The antibody was conjugated to BD Horizon™ BV421 which is part of the BD Horizon Brilliant™ Violet family of dyes
• With an Ex Max of 407nm and Em Max at 421nm, BD Horizon BV421 can be excited by the violet laser and detected in the standard Pacific Blue™ filter set (eg, 450/50nm filter)
• BD Horizon BV421 conjugates are very bright, often exhibiting a 10 fold improvement in brightness compared to Pacific Blue conjugates
• BV421 is a polymer-based dye excited by the violet laser and is one of the brightest fluorochromes offered by BD Biosciences
• Conjugates are typically 10 times brighter than Pacific Blue™ conjugates and are often as bright as or brighter than PE conjugates
• Due to nearly identical excitation and emission properties but different spillover characteristics, BD Horizon BV421, Pacific Blue™, and BD Horizon™ V450 cannot be used simultaneously.