CD137 Ligand Mouse anti-Human, Brilliant Violet 711, Clone: C65-485, BD Optibuild™

Description

• The C65-485 monoclonal antibody specifically recognizes the type II membrane protein known as 4-1BB Ligand (4-1BB-L) or CD137 Ligand (CD137L)
• This molecule belongs to the TNF superfamily and is encoded by TNFSF9 /(Tumor necrosis factor superfamily member 9)
• CD137 Ligand has been reported to have costimulatory function on activated T cells
• Reports suggest that stimulation of activated T cells via CD137 Ligand does not require the presence of other costimulatory molecules, including CD28
• Some studies have reported human CD137 Ligand to be expressed preferentially on primary B cells and B-cell lines
• The C65-485 antibody is able to react with recombinant human 4-1BB Ligand (rh4-1BB-L/CD137L) bound to CD137 (4-1BB receptor) expressed on phytohemagglutinin (PHA)-stimulated PBMC
• The antibody was conjugated to BD Horizon™ BV711 which is part of the BD Horizon Brilliant™ Violet family of dyes
• This dye is a tandem fluorochrome of BD Horizon BV421 with an Ex Max of 405nm and an acceptor dye with an Em Max at 711nm
• BD Horizon BV711 can be excited by the violet laser and detected in a filter used to detect Cy™5.5 / Alexa Fluor™ 700-like dyes (eg, 712/20nm filter)
• Due to the excitation and emission characteristics of the acceptor dye, there may be moderate spillover into the Alexa Fluor™ 700 and PerCP-Cy5.5 detectors
• However, the spillover can be corrected through compensation as with any other dye combination
• BV711 is a tandem fluorochrome of BD Horizon BV421 and an acceptor dye with an emission maximum at 711 nm
• This dye offers a very bright choice for the violet laser
• Due to the excitation and emission characteristics of the acceptor dye, there may be moderate spillover into the Alexa Fluor™ 700 and PerCP-Cy5.5 detectors
• BV711 will also have moderate spillover into the BD Horizon Brilliant™ Violet 786 detector.