Platelet GPVI Mouse anti-Human, Brilliant Violet 421, Clone: HY101 (also known as HY 101; HY-101), BD Optibuild™

Description

• The HY101 monoclonal antibody specifically binds to a collagen receptor, Platelet Glycoprotein VI (GPVI or GP6)
• GPVI is a 58-kD platelet membrane glycoprotein that belongs to the immunoglobulin superfamily
• Upon blood vessel wall injury and consequent endothelial cell damage, platelets collect upon the exposed collagen fibers present in the subendothelial matrix
• The platelet and collagen interaction leads to activated platelet aggregation and thrombus formation
• Although normally serving a protective function in clot formation that limits blood loss, the platelet GPVI/collagen interaction may also play a role in myocardial infarction and stroke which are associated with the rupture of atherosclerotic plaques
• The antibody was conjugated to BD Horizon™ BV421 which is part of the BD Horizon Brilliant™ Violet family of dyes
• With an Ex Max of 407nm and Em Max at 421nm, BD Horizon BV421 can be excited by the violet laser and detected in the standard Pacific Blue™ filter set (eg, 450/50nm filter)
• BD Horizon BV421 conjugates are very bright, often exhibiting a 10 fold improvement in brightness compared to Pacific Blue conjugates
• BV421 is a polymer-based dye excited by the violet laser and is one of the brightest fluorochromes offered by BD Biosciences
• Conjugates are typically 10 times brighter than Pacific Blue™ conjugates and are often as bright as or brighter than PE conjugates
• Due to nearly identical excitation and emission properties but different spillover characteristics, BD Horizon BV421, Pacific Blue™, and BD Horizon™ V450 cannot be used simultaneously.