Platelet GPVI Mouse anti-Human, Brilliant Violet 711, Clone: HY101 (also known as HY 101; HY-101), BD Optibuild™

Description

• The HY101 monoclonal antibody specifically binds to a collagen receptor, Platelet Glycoprotein VI (GPVI or GP6)
• GPVI is a 58-kD platelet membrane glycoprotein that belongs to the immunoglobulin superfamily
• Upon blood vessel wall injury and consequent endothelial cell damage, platelets collect upon the exposed collagen fibers present in the subendothelial matrix
• The platelet and collagen interaction leads to activated platelet aggregation and thrombus formation
• Although normally serving a protective function in clot formation that limits blood loss, the platelet GPVI/collagen interaction may also play a role in myocardial infarction and stroke which are associated with the rupture of atherosclerotic plaques
• The antibody was conjugated to BD Horizon™ BV711 which is part of the BD Horizon Brilliant™ Violet family of dyes
• This dye is a tandem fluorochrome of BD Horizon BV421 with an Ex Max of 405nm and an acceptor dye with an Em Max at 711nm
• BD Horizon BV711 can be excited by the violet laser and detected in a filter used to detect Cy™5.5 / Alexa Fluor™ 700-like dyes (eg, 712/20nm filter)
• Due to the excitation and emission characteristics of the acceptor dye, there may be moderate spillover into the Alexa Fluor™ 700 and PerCP-Cy5.5 detectors
• However, the spillover can be corrected through compensation as with any other dye combination
• BV711 is a tandem fluorochrome of BD Horizon BV421 and an acceptor dye with an emission maximum at 711 nm
• This dye offers a very bright choice for the violet laser
• Due to the excitation and emission characteristics of the acceptor dye, there may be moderate spillover into the Alexa Fluor™ 700 and PerCP-Cy5.5 detectors
• BV711 will also have moderate spillover into the BD Horizon Brilliant™ Violet 786 detector.