Vγ9 TCR Mouse anti-Human, BUV395, Clone: B3, BD Optibuild™

Description

• The B3 monoclonal antibody specifically recognizes the human variableγ9 subunit (Vγ9) ofγ/δ T- cell receptor (TCR)
• Theγ/δTCR is composed of two disulfide-linked glycoproteins
• The gamma chain is approximately 45-60 kD and is detected on a small subset peripheral blood T cells and thymocytes
• The majority of normal peripheral bloodγ/δT cells express a Vγ9+/Vδ2+ phenotype
• The reason for this selection in the T-cell repertoire is not well understood
• The antibody was conjugated to BD Horizon™ BUV395 which is part of the BD Horizon Brilliant™ Ultraviolet family of dyes
• This dye has been exclusively developed by BD Biosciences to have minimal spillover into other detectors, making it an optimal choice for multicolor flow cytometry
• With an Ex Max at 348 nm and an Em Max at 395 nm, BD Horizon BUV395 can be excited with a 355 nm laser and detected with a 379/28 filter
• BUV395 is a UV-excitable dye that has been developed exclusively by BD Biosciences
• With an excitation max of 348 nm and emission max of 395 nm, BUV395 can be excited by the 355nm laser and detected in a 379/28 filter
• This dye is optimal for multicolor flow cytometry because it has little to no spillover into other detectors.