CD203c Mouse anti-Human, Brilliant Violet 650, Clone: NP4D6, BD Optibuild™

Description

• The NP4D6 monoclonal antibody specifically binds to CD203c
• CD203c is a type II transmembrane glycoprotein that is a member of the E-NNP family of ectoenzymes
• CD203c is also known as ectonucleotide pyrophosphatase/phosphodiesterase 3 (E-NPP3, ENPP3) due to its capacity to hydrolyze phosphodiester and phosphosulfate bonds in a variety of molecules including deoxynucleotides, nucleoside phosphates, and nicotinamide adenine dinucleotide
• CD203c is otherwise known as Phosphodiesterase-1 (PD-1, PD-I beta), B10, or gp130RB13-6
• CD203c is expressed by basophils and mast cells
• Basophils increase CD203c expression following activation with allergens or antibodies that crosslink cytophilic IgE
• Thus, CD203c serves as a useful flow cytometric marker for basophil activation and the detection and analysis of type 1 allergic responses
• IgE-receptor cross-linking results in CD203c upregulation and overexpression on neoplastic mast cells in cases of systemic mastocytosis
• The antibody was conjugated to BD Horizon™ BV650 which is part of the BD Horizon Brilliant™ Violet family of dyes
• This dye is a tandem fluorochrome of BD Horizon BV421 with an Ex Max of 405nm and an acceptor dye with an Em Max at 650nm
• BD Horizon BV650 can be excited by the violet laser and detected in a filter used to detect APC-like dyes (eg, 660/20nm filter)
• Due to the excitation and emission characteristics of the acceptor dye, there will be spillover into the APC and Alexa Fluor™ 700 detectors
• However, the spillover can be corrected through compensation as with any other dye combination
• BV650 is a tandem fluorochrome of BD Horizon BV421 and an acceptor dye with an emission maximum at 650 nm
• Due to the excitation and emission characteristics of the acceptor dye, there will be spillover into the APC and Alexa Fluor™ 700 detectors
• BV650 will have moderate spillover into the BD Horizon™ BV711 detector.