CD203c Mouse anti-Human, BUV395, Clone: NP4D6, BD Optibuild™

Description

• The NP4D6 monoclonal antibody specifically binds to CD203c
• CD203c is a type II transmembrane glycoprotein that is a member of the E-NNP family of ectoenzymes
• CD203c is also known as ectonucleotide pyrophosphatase/phosphodiesterase 3 (E-NPP3, ENPP3) due to its capacity to hydrolyze phosphodiester and phosphosulfate bonds in a variety of molecules including deoxynucleotides, nucleoside phosphates, and nicotinamide adenine dinucleotide
• CD203c is otherwise known as Phosphodiesterase-1 (PD-1, PD-I beta), B10, or gp130RB13-6
• CD203c is expressed by basophils and mast cells
• Basophils increase CD203c expression following activation with allergens or antibodies that crosslink cytophilic IgE
• Thus, CD203c serves as a useful flow cytometric marker for basophil activation and the detection and analysis of type 1 allergic responses
• IgE-receptor cross-linking results in CD203c upregulation and overexpression on neoplastic mast cells in cases of systemic mastocytosis
• The antibody was conjugated to BD Horizon™ BUV395 which is part of the BD Horizon Brilliant™ Ultraviolet family of dyes
• This dye has been exclusively developed by BD Biosciences to have minimal spillover into other detectors, making it an optimal choice for multicolor flow cytometry
• With an Ex Max at 348 nm and an Em Max at 395 nm, BD Horizon BUV395 can be excited with a 355 nm laser and detected with a 379/28 filter
• BUV395 is a UV-excitable dye that has been developed exclusively by BD Biosciences
• With an excitation max of 348 nm and emission max of 395 nm, BUV395 can be excited by the 355nm laser and detected in a 379/28 filter
• This dye is optimal for multicolor flow cytometry because it has little to no spillover into other detectors.