CD47 Mouse anti-Human, BUV395, Clone: B6H12, BD Optibuild™

Description

• The B6H12 monoclonal antibody specifically binds to CD47, a 42-52 kDa N-linked glycan protein
• CD47 antigen, also known as integrin-associated protein (IAP), is expressed on all hematopoietic cells, including leukocytes, platelets and erythrocytes
• It is also expressed on epithelial cells, endothelial cells, fibroblasts and many tumor cell lines
• CD47 may play a role as a signal transducer in the regulation of cation fluxes across cell membranes and in the chemotactic and adhesive interactions of leukocytes with endothelial cells
• The B6H12 antibody is capable of blocking phagocytosis of microparticles by peripheral blood granulocytes
• It has also been reported to induce proliferation of CD3-activated T cells The antibody was conjugated to BD Horizon™ BUV395 which is part of the BD Horizon Brilliant™ Ultraviolet family of dyes
• This dye has been exclusively developed by BD Biosciences to have minimal spillover into other detectors, making it an optimal choice for multicolor flow cytometry
• With an Ex Max at 348 nm and an Em Max at 395 nm, BD Horizon BUV395 can be excited with a 355 nm laser and detected with a 379/28 filter
• BUV395 is a UV-excitable dye that has been developed exclusively by BD Biosciences
• With an excitation max of 348 nm and emission max of 395 nm, BUV395 can be excited by the 355nm laser and detected in a 379/28 filter
• This dye is optimal for multicolor flow cytometry because it has little to no spillover into other detectors.