CD89 Mouse anti-Human, Brilliant Violet 711, Clone: A59, BD Optibuild™

Description

• The A59 monoclonal antibody specifically recognizes CD89
• CD89 is the Fc receptor for IgA (Fc α R), a 55-75 kDa glycoprotein expressed exclusively on cells of granulocytic and monocyte/macrophage lineages in peripheral blood, but not on lymphocytes
• It is also expressed on promyelocytes in bone marrow and in the cytoplasm of fixed monocytes
• This mAb does not block IgA binding and is more efficient than native IgA ligands in the isolation of Fc α R molecules
• Fc α R plays a role in triggering phagocytosis and respiratory burst
• The antibody was conjugated to BD Horizon™ BV711 which is part of the BD Horizon Brilliant™ Violet family of dyes
• This dye is a tandem fluorochrome of BD Horizon BV421 with an Ex Max of 405nm and an acceptor dye with an Em Max at 711nm
• BD Horizon BV711 can be excited by the violet laser and detected in a filter used to detect Cy™5.5 / Alexa Fluor™ 700-like dyes (eg, 712/20nm filter)
• Due to the excitation and emission characteristics of the acceptor dye, there may be moderate spillover into the Alexa Fluor™ 700 and PerCP-Cy5.5 detectors
• However, the spillover can be corrected through compensation as with any other dye combination
• BV711 is a tandem fluorochrome of BD Horizon BV421 and an acceptor dye with an emission maximum at 711 nm
• This dye offers a very bright choice for the violet laser
• Due to the excitation and emission characteristics of the acceptor dye, there may be moderate spillover into the Alexa Fluor™ 700 and PerCP-Cy5.5 detectors
• BV711 will also have moderate spillover into the BD Horizon Brilliant™ Violet 786 detector.