CD49c Mouse anti-Human, Brilliant Violet 711, Clone: C3 II.1, BD Optibuild™

Description

• The C3 II.1 monoclonal antibody specifically recognizes CD49c which is also known as Integrin alpha 3 (α3 integrin)
• CD49c is encoded by ITGA3 /(Integrin subunit alpha 3)
• CD49c isa 150 kDa type I transmembrane glycoprotein which non-covalently associates with integrin β1 (CD29) to form the α3β1 (CD49c/CD29, VLA-3) complex
• CD49c is expressed mostly on endothelial and epithelial cells (basal epidermal layers)
• It is not expressedon platelets and is expressed weakly on peripheral blood leucocytes, including T cells, B cells, and monocytes
• The CD49c/CD29 complex serves as an adhesive receptor for kalinin or epiligrin
• This interaction may be important for thymocyte interaction with thymic epithelium
• The antibody was conjugated to BD Horizon™ BV711 which is part of the BD Horizon Brilliant™ Violet family of dyes
• This dye is a tandem fluorochrome of BD Horizon BV421 with an Ex Max of 405nm and an acceptor dye with an Em Max at 711nm
• BD Horizon BV711 can be excited by the violet laser and detected in a filter used to detect Cy™5.5 / Alexa Fluor™ 700-like dyes (eg, 712/20nm filter)
• Due to the excitation and emission characteristics of the acceptor dye, there may be moderate spillover into the Alexa Fluor™ 700 and PerCP-Cy5.5 detectors
• However, the spillover can be corrected through compensation as with any other dye combination
• BV711 is a tandem fluorochrome of BD Horizon BV421 and an acceptor dye with an emission maximum at 711 nm
• This dye offers a very bright choice for the violet laser
• Due to the excitation and emission characteristics of the acceptor dye, there may be moderate spillover into the Alexa Fluor™ 700 and PerCP-Cy5.5 detectors
• BV711 will also have moderate spillover into the BD Horizon Brilliant™ Violet 786 detector.