CD49c Mouse anti-Human, Brilliant Violet 605, Clone: C3 II.1, BD Optibuild™

Description

• The C3 II.1 monoclonal antibody specifically recognizes CD49c which is also known as Integrin alpha 3 (α3 integrin)
• CD49c is encoded by ITGA3 /(Integrin subunit alpha 3)
• CD49c isa 150 kDa type I transmembrane glycoprotein which non-covalently associates with integrin β1 (CD29) to form the α3β1 (CD49c/CD29, VLA-3) complex
• CD49c is expressed mostly on endothelial and epithelial cells (basal epidermal layers)
• It is not expressedon platelets and is expressed weakly on peripheral blood leucocytes, including T cells, B cells, and monocytes
• The CD49c/CD29 complex serves as an adhesive receptor for kalinin or epiligrin
• This interaction may be important for thymocyte interaction with thymic epithelium
• This antibody is conjugated to BD Horizon™ BV605 which is part of the BD Horizon Brilliant™ Violet family of dyes
• With an Ex Max of 407nm and Em Max of 602nm, BD Horizon BV605 can be excited by a violet laser and detected with a standard 610/20nm filter set
• BD Horizon BV605 is a tandem fluorochrome of BD Horizon BV421 and an acceptor dye with an Em max at 605nm
• Due to the excitation of the acceptor dye by the green (532 nm) and yellow-green (561 nm) lasers, there will be significant spillover into the PE and BD Horizon PE-CF594 detectors off the green or yellow-green lasers
• BD Horizon BV605 conjugates are very bright, often exhibiting brightness equivalent to PE conjugates and can be used as a third color off of the violet laser
• BV605 is a tandem fluorochrome that combines BD Horizon BV421 and Cy™3.5
• These conjugates are very bright, exhibiting similar brightness to equivalent PE conjugates
• Due to the excitation of Cy3.5 by the green (532nm) and yellow-green (561nm) lasers, there will be significant spillover of the BD Horizon BV605 signal into the PE and BD Horizon PE-CF594 detectors off the green or yellow-green lasers.