CD49c Mouse anti-Human, Brilliant Violet 421, Clone: C3 II.1, BD Optibuild™

Description

• The C3 II.1 monoclonal antibody specifically recognizes CD49c which is also known as Integrin alpha 3 (α3 integrin)
• CD49c is encoded by ITGA3 /(Integrin subunit alpha 3)
• CD49c isa 150 kDa type I transmembrane glycoprotein which non-covalently associates with integrin β1 (CD29) to form the α3β1 (CD49c/CD29, VLA-3) complex
• CD49c is expressed mostly on endothelial and epithelial cells (basal epidermal layers)
• It is not expressedon platelets and is expressed weakly on peripheral blood leucocytes, including T cells, B cells, and monocytes
• The CD49c/CD29 complex serves as an adhesive receptor for kalinin or epiligrin
• This interaction may be important for thymocyte interaction with thymic epithelium
• The antibody was conjugated to BD Horizon™ BV421 which is part of the BD Horizon Brilliant™ Violet family of dyes
• With an Ex Max of 407nm and Em Max at 421nm, BD Horizon BV421 can be excited by the violet laser and detected in the standard Pacific Blue™ filter set (eg, 450/50nm filter)
• BD Horizon BV421 conjugates are very bright, often exhibiting a 10 fold improvement in brightness compared to Pacific Blue conjugates
• BV421 is a polymer-based dye excited by the violet laser and is one of the brightest fluorochromes offered by BD Biosciences
• Conjugates are typically 10 times brighter than Pacific Blue™ conjugates and are often as bright as or brighter than PE conjugates
• Due to nearly identical excitation and emission properties but different spillover characteristics, BD Horizon BV421, Pacific Blue™, and BD Horizon™ V450 cannot be used simultaneously.