V Alp 8.3 TCR Mouse, anti-Mouse, R718, Clone: KT50, BD OptiBuild™

Description

• The KT50 antibody reacts with some members of the Vα 8 T-cell Receptor (TCR) subfamily of mice having the a, b, c, and d haplotypes of the Tcra gene complex (e.g., all strains tested)
• It recognizes an epitope in the CDR1 of Vα 8.3, but not Vα 8.2, TCR subfamily member, as does the B21.14 mAb (Cat
• no
• 553374)
• Site-directed mutagenesis has identified three amino acids which are necessary for antibody reactivity and which are unique to Vα 8.3 among the five functional Vα 8 TCR subfamily members
• On a common H-2[k] background, the frequency of Vα 8.3 TCR-bearing T lymphocytes is higher in Tcra[a/a] mice than in Tcra[a/b] mice
• Furthermore, studies of congenic strains suggest that CD8+ Vα 8.3 TCR-bearing T lymphocytes undergo negative selection in mice expressing MHC class I antigens of the H-2[d] haplotype.