FR4 Mouse, anti-Mouse, R718, Clone: 12A5, BD OptiBuild™

Description

• The monoclonal antibodies TH6 and 12A5 recognize Folate Receptor 4 (FR4), also known as the membrane folate-binding protein 3 (FBP3)
• FR4 is a heavily glycosylated 35 kD receptor expressed exclusively in lymphoid tissue and an isoform of the family of receptors that recognize the essential nutrient folic acid
• Natural T regulatory cells constitutively express high levels of FR4
• Differential expression of FR4 in combination with CD25 can distinguish four functionally distinct CD4+ T cell subpopulations| Natural Tregs, effector T cells, memory-like T cells and Naïve T cells
• FR4hi CD25+ expressing CD4+ T cells also express high amounts of Foxp3, GITR and CTLA-4
• Monoclonal antibody TH6 and 12A5 stained CD25+CD4+ T cells at a higher level than other CD4+ or CD8+ T cells
• In addition, in vivo injection of TH6 monoclonal antibody reduced the number of CD25+CD4+ T cells and CD25-CD4+ T cells in peripheral blood
• Clone 12A5 has been demonstrated to work in western blot
• Clones TH6 and 12A5 do not block one another in a flow cytometric assay.