CD171 Mouse, anti-Human, R718, Clone: 5G3, BD OptiBuild™

Description

• Neurite adhesion molecule L1 has been implicated in neuron-neuron and neuron-Schwann cell adhesion in vertebrates
• L1-like molecules, found in mouse, rat, chicken, and human, promote axonal elongation and may also play a role in regeneration of axons after injury
• Molecular cloning data suggest 87% amino acid identity between mouse and human L1 molecules
• 5G3 antigen (Ag), originally defined by monoclonal antibody 5G3, is considered to be the human homologue of mouse L1
• The 5G3 antibody was developed against a human neuroblastoma cell line to use as a probe for the elucidating the biological characteristics of neuroblastoma
• 5G3 specifically recognizes a neuroblastoma target glycoprotein antigen of 215 kDa and its 200 kDa precursor
• The 215 kDa molecule is expressed on the cell surface| whereas the 200 kDa precursor is shed from the cell surface
• The 215 and 200 kDa species also differ in their posttranslational modification patterns
• The 5G3 antibody has been used as a marker for neuroblastoma, and to purify 5G3 Ag from normal adult human brain
• The antibody recognizes human L1 on human neuroblastoma cell lines and tissues
• Reactivity has been tested on a variety of malignant and normal tissues
• Squamous lung, squamous skin, and osteogenic sarcoma cell lines were positive, as were two out of eight melanoma cell lines tested
• A variety of other cell lines and tumor tissues tested negative
• 5G3 did not react with either T or B lymphoblastoid cell lines or a fibroblast cell line
• Among all the normal tissues tested, mAb 5G3 reacted only with cerebellum
• The molecular masses observed using mAb 5G3 may vary among immunoprecipitation isolates
• In normal human cerebellum, 5G3 Ag migrated as a 190/200 kDa doublet, 140 kDa band with minor bands at 80 and 65 kDa
• 5G3 Ag isolated from SK-N-AS cells migrates as 200 to 215 kDa bands, or as a diffuse band ranging from 200 to 215 kDa
• Additional bands have been described at 140 to 150 kDa in SK-N-AS cells
• Only the 200 kDa band has been detected in culture media from SK-N-AS cells.