IGF2R Monoclonal Antibody (2G11), Invitrogen™

Description

• MA1-066 detects mannose 6-phospate receptor (MPR) from human, rat, monkey and bovine tissues
• This antibody does not detect Chinese hamster ovary cell MPR
• MA1-066 has been successfully used in Western blot, immunofluorescence, EM immunocytochemistry and immunoprecipitation procedures
• By Western blot, this antibody detects a ∽300 kDa protein representing MPR in HeLa cell extract under non-reducing conditions
• Immunofluorescence staining of MPR in HeLa cells with MA1-066 results in perinuclear staining
• MA1-066 immunogen is purified bovine ∽300 kDa Cl-MPR
• This antibody is shown to recognize an epitope in the extracellular domain of MPR
• Lysosomal enzymes containing one or two mannose 6-phosphate (man6P) moieties are moved about in the cell by two distinct but interconnected cycles by means of approximately 300 kDa cation independent mannose 6-phospate receptors (CI-MPR)
• The biosynthetic cycle refers to the MPR transport of newly synthesized lysosomal enzymes from the trans Golgi network to late endosomes or early lysosomes
• The endocytic cycle transports extracellular lysosomal enzymes from the plasma membrane via clathrin-coated vesicles to early endosomes
• The entire pool of MPRs cycles between these cellular compartments every 3 hours
• The steady state distribution of MPR's is predominantly within late endosomes, fewer in the trans Golgi network and approximately 10 % at cell surface
• In addition to its man6P binding activity, the MPR contains a separate binding site for the type II insulin-like growth factor and is capable of binding both man6P and IGF-II simultaneously
• An approximately 240 kDa soluble, truncated form, representing the extracellular domain of the protein, has also been found circulating in serum and is capable of binding both ligands.