GCAP1 Monoclonal Antibody (G2), Invitrogen™

Description

• MA1-724 detects GCAP-1 from human, non-human primate and bovine samples
• This antibody is specific for GCAP-1 and does not react with other isotypes
• MA1-724 has been successfully used in Western blot procedures
• By Western blot, this antibody detects a ∽23 kDa protein representing GCAP-1 protein on bovine rod outer segment membranes
• The MA1-724 immunogen is bacterial expressed full length GCAP-1
• Guanylate cyclase-activating protein is a l Ca(2+)-binding protein that upregulates synthesis of cGMP in photoreceptors
• The known mammalian GCAPs are more than 90% similar, consisting of 201 to 205 amino acids, and containing 3 identically conserved Ca(2+)-binding sites
• The GUCA1A gene, also termed GCAP1, is transcribed into a single 1.7-kb mRNA species detectable only in the retina
• In a 4-generation British family with typical clinical features of autosomal dominant cone dystrophy a tyr99-to-cys mutation) in the GUCA1A gene has been identified
• Another family with a pro50-to-leu mutation in GUCA1A demonstrated phenotypic variability ranging from mild photophobia to rod-cone dystrophy
• The mutant protein could activate guanylate cyclase 1 (GUCY2D) and displayed similar calcium sensitivity to wildtype protein
• However, there was a marked increase in the susceptibility to protease degradation and a reduction in the thermal stability of the pro50-to-leu mutation, which may depress cellular concentration and thereby contribute to retinal cell mortality.