MABF210725

Chlamydial LPS, Mouse anti-Bacteria, Clone: EVI-H1, Millipore Sigma™

Manufacturer: Fischer Scientific

Select a Size

Pack Size SKU Availability Price
Each of 1 MABF210725-Each-of-1 In Stock ₹ 16,456.61

MABF210725 - Each of 1

₹ 16,456.61

In Stock

Quantity

1

Base Price: ₹ 16,456.61

GST (18%): ₹ 2,962.19

Total Price: ₹ 19,418.80

Antigen

Chlamydial LPS

Classification

Monoclonal

Conjugate

Unconjugated

Formulation

Purified mouse monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Host Species

Mouse

Purification Method

Protein G purified

Regulatory Status

RUO

Primary or Secondary

Primary

Target Species

Bacteria

Form

Purified

Applications

Immunocytochemistry, Immunofluorescence, Immunomicroscopy, Western Blot

Clone

EVI-H1

Dilution

Immunofluorescence Analysis: A representative lot detected Chlamydial LPS in Immunofluorescence applications (Su, H., et. al. (2000). Infect Immun. 68(1):192-6; Wolf, K., et. al. (2001). Infect Immun. 69(5):3082-91).Immunocytochemistry Analysis: A representative lot detected Chlamydial LPS in Immunocytochemistry applications (Wolf, K., et. al. (2001). Infect Immun. 69(5):3082-91).Electron Microscopy Analysis: A representative lot detected Chlamydial LPS with Trasnsmission Electron Microscopy applications (Wolf, K., et. al. (2001). Infect Immun. 69(5):3082-91).

Gene Alias

Chlamydial lipopolysaccharide;Chlamydia trachomatis LPS

Immunogen

Formalin-killed elementary bodies of the Chlamydia trachomatis L2 serovar.

Quantity

25 μg

Research Discipline

Inflammation & Immunology

Test Specificity

Clone EVH-H1 detects lipopolysaccharide from Chlamydia genus.

Content And Storage

Stable for 1 year at 2-8°C from date of receipt.

Isotype

IgG2a

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Description

  • Anti-Chlamydial LPS, clone EVI-H1, Cat
  • No
  • MABF2107, is a mouse monoclonal antibody that detects Chlamydial lipopolysaccharide (LPS) and has been tested for use in Electron Microscopy, Immunocytochemistry, Immunofluorescence, and Western Blotting
  • Chlamydia trachomatis is a Gram-negative bacterium that is responsible to sexually transmitted diseases leading to pelvic inflammatory disease, ectopic pregnancy, infertility, and outbreaks of trachoma-associated blindness and lymphogranuloma venereum (LGV)
  • Chlamydia trachomatis consists of eighteen different serological variants (serovars) that include a few subvariants
  • These are identified based on serological reactivity of the epitopes on their outer membrane
  • Intracellularly chlamydia replicates within a vacuole
  • Chlamydia infection is initiated with the expression of a chlamydial early gene product(s), which isolate the inclusion from the endocytic-lysosomal pathway and makes it fusogenic with sphingomyelin-containing exocytic vesicles
  • This change in vesicular interaction allows the delivery of the vacuole to the peri-Golgi region of the host cell
  • Antigens from all members of the Chlamydia genus display heat resistance and sensitivity to oxidation by sodium periodate
  • Lipopolysaccharides (LPS) of Chlamydia consists of at least three antigen domains, two of which are shared by the LPS of certain other gram-negative organisms and one that is unique only to chlamydial LPS
  • It has been reported that the quantitative yields of chlamydial LPS can be achieved when elementary bodies (EBs) are first reduced and alkylated prior to extraction with hot phenol-water
  • (Ref.: Caldwell, HD., and Hitchcock, PJ
  • (1984)
  • Infect
  • Immun
  • (44(2); 306-314; Scidmore, MA., et al
  • (1996)
  • Infect
  • Immun
  • 64(12); 5366-5372; Turingan, RS et al
  • (2017)
  • PLoS ONE 12(5): e0178653).

Compare Similar Items

Show Difference

Img

Fischer Scientific

MABF210725

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Antigen:
Chlamydial LPS

Classification:
Monoclonal

Conjugate:
Unconjugated

Formulation:
Purified mouse monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Host Species:
Mouse

Purification Method:
Protein G purified

Regulatory Status:
RUO

Primary or Secondary:
Primary

Target Species:
Bacteria

Form:
Purified

Applications:
Immunocytochemistry, Immunofluorescence, Immunomicroscopy, Western Blot

Clone:
EVI-H1

Dilution:
Immunofluorescence Analysis: A representative lot detected Chlamydial LPS in Immunofluorescence applications (Su, H., et. al. (2000). Infect Immun. 68(1):192-6; Wolf, K., et. al. (2001). Infect Immun. 69(5):3082-91).Immunocytochemistry Analysis: A representative lot detected Chlamydial LPS in Immunocytochemistry applications (Wolf, K., et. al. (2001). Infect Immun. 69(5):3082-91).Electron Microscopy Analysis: A representative lot detected Chlamydial LPS with Trasnsmission Electron Microscopy applications (Wolf, K., et. al. (2001). Infect Immun. 69(5):3082-91).

Gene Alias:
Chlamydial lipopolysaccharide;Chlamydia trachomatis LPS

Immunogen:
Formalin-killed elementary bodies of the Chlamydia trachomatis L2 serovar.

Quantity:
25 μg

Research Discipline:
Inflammation & Immunology

Test Specificity:
Clone EVH-H1 detects lipopolysaccharide from Chlamydia genus.

Content And Storage:
Stable for 1 year at 2-8°C from date of receipt.

Isotype:
IgG2a

Img

Fischer Scientific

MABF2108100

--


Antigen:
Chlamydial HSP60

Classification:
Monoclonal

Conjugate:
Unconjugated

Formulation:
Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Host Species:
Mouse

Purification Method:
Protein G purified

Regulatory Status:
RUO

Primary or Secondary:
Primary

Target Species:
Bacteria

Form:
Purified

Applications:
ELISA, Immunocytochemistry, Immunofluorescence, Western Blot

Clone:
A57-B9

Dilution:
Immunocytochemistry Analysis: A representative lot detected Chlamydial HSP60 in Immunocytochemistry applications (Yuan, Y., et. al. (1992). Infect Immun. 60(6):2288-96).Immunofluorescence Analysis: A representative lot detected Chlamydial HSP60 in Immunofluorescence applications (Southern, T., et. al. (2012). Clin Vaccine Immunol. 19(11):1864-9).ELISA Analysis: A representative lot detected Chlamydial HSP60 in ELISA applications (Morrison, S.G., et. al. (2005). J Immunol. 175(11):7536-42).Western Blotting Analysis: A representative lot detected chlamydial HSP60 in Western Blotting applications (Hechard, C., et. al. (2004). J Med Microbiol. 53(Pt 9):861-8; LaVerda, D., et. al. (1997). J Clin Microbiol. 35(5):1209-15; Southern, T., et. al. (2012). Clin Vaccine Immunol. 19(11):1864-9; Yuan, Y., et. al. (1992). Infect Immun. 60(6):2288-96).

Gene Alias:
Chlamydia heat shock protein 60

Immunogen:
Recombinant Chlamydia trachomatis serovar A HSP60 emulsified in complete Freund adjuvant.

Quantity:
100 μg

Research Discipline:
Inflammation & Immunology

Test Specificity:
Clone A57-B9 detects Chlamydial HSP60. It targets an epitope with in 144 amino acids in the carboxy terminal of HSP60.

Content And Storage:
Stable for 1 year at 2-8°C from date of receipt.

Isotype:
IgG1 κ

Img

Fischer Scientific

MABF210825

--


Antigen:
Chlamydial HSP60

Classification:
Monoclonal

Conjugate:
Unconjugated

Formulation:
Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Host Species:
Mouse

Purification Method:
Protein G purified

Regulatory Status:
RUO

Primary or Secondary:
Primary

Target Species:
Bacteria

Form:
Purified

Applications:
ELISA, Immunocytochemistry, Immunofluorescence, Western Blot

Clone:
A57-B9

Dilution:
Immunocytochemistry Analysis: A representative lot detected Chlamydial HSP60 in Immunocytochemistry applications (Yuan, Y., et. al. (1992). Infect Immun. 60(6):2288-96).Immunofluorescence Analysis: A representative lot detected Chlamydial HSP60 in Immunofluorescence applications (Southern, T., et. al. (2012). Clin Vaccine Immunol. 19(11):1864-9).ELISA Analysis: A representative lot detected Chlamydial HSP60 in ELISA applications (Morrison, S.G., et. al. (2005). J Immunol. 175(11):7536-42).Western Blotting Analysis: A representative lot detected chlamydial HSP60 in Western Blotting applications (Hechard, C., et. al. (2004). J Med Microbiol. 53(Pt 9):861-8; LaVerda, D., et. al. (1997). J Clin Microbiol. 35(5):1209-15; Southern, T., et. al. (2012). Clin Vaccine Immunol. 19(11):1864-9; Yuan, Y., et. al. (1992). Infect Immun. 60(6):2288-96).

Gene Alias:
Chlamydia heat shock protein 60

Immunogen:
Recombinant Chlamydia trachomatis serovar A HSP60 emulsified in complete Freund adjuvant.

Quantity:
25 μg

Research Discipline:
Inflammation & Immunology

Test Specificity:
Clone A57-B9 detects Chlamydial HSP60. It targets an epitope with in 144 amino acids in the carboxy terminal of HSP60.

Content And Storage:
Stable for 1 year at 2-8°C from date of receipt.

Isotype:
IgG1 κ

Img

Fischer Scientific

MABF21151MI

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Antigen:
NKR-P1B (CD161b)

Classification:
Monoclonal

Conjugate:
Unconjugated

Formulation:
__

Host Species:
Mouse

Purification Method:
Protein G Purified

Regulatory Status:
__

Primary or Secondary:
Primary

Target Species:
Mouse

Form:
Purified

Applications:
Flow Cytometry, Functional Assay, Immunoprecipitation, Western Blot

Clone:
2D12

Dilution:
__

Gene Alias:
Killer cell lectin-like receptor subfamily B member 1B allele B;CD161 antigen-like family member B;Inhibitory receptor NKR-P1B;Lymphocyte antigen 55d;Ly-55d;Natural killer cell surface protein NKR-P1B allele B6;CD161b

Immunogen:
Mouse BaF/3 cells (murine IL-3-dependent pro-B cell line) expressing mouse NKR-P1B protein.

Quantity:
100 μL

Research Discipline:
Inflammation and Immunology

Test Specificity:
Clone 2D12 is a mouse monoclonal antibody that detects Natural killer cell surface protein NKR-P1B allele B6 (NKR-P1B) in murine species. It does not react with Nkrp1a, Nkrp1c, and Nkrp1f.

Content And Storage:
Stable for 1 year at -20°C from date of receipt.

Isotype:
IgG2a λ