MABF210725
Chlamydial LPS, Mouse anti-Bacteria, Clone: EVI-H1, Millipore Sigma™
Manufacturer: Fischer Scientific
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | MABF210725-Each-of-1 | In Stock | ₹ 16,456.61 |
MABF210725 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 16,456.61
GST (18%): ₹ 2,962.19
Total Price: ₹ 19,418.80
Antigen
Chlamydial LPS
Classification
Monoclonal
Conjugate
Unconjugated
Formulation
Purified mouse monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Host Species
Mouse
Purification Method
Protein G purified
Regulatory Status
RUO
Primary or Secondary
Primary
Target Species
Bacteria
Form
Purified
Applications
Immunocytochemistry, Immunofluorescence, Immunomicroscopy, Western Blot
Clone
EVI-H1
Dilution
Immunofluorescence Analysis: A representative lot detected Chlamydial LPS in Immunofluorescence applications (Su, H., et. al. (2000). Infect Immun. 68(1):192-6; Wolf, K., et. al. (2001). Infect Immun. 69(5):3082-91).Immunocytochemistry Analysis: A representative lot detected Chlamydial LPS in Immunocytochemistry applications (Wolf, K., et. al. (2001). Infect Immun. 69(5):3082-91).Electron Microscopy Analysis: A representative lot detected Chlamydial LPS with Trasnsmission Electron Microscopy applications (Wolf, K., et. al. (2001). Infect Immun. 69(5):3082-91).
Gene Alias
Chlamydial lipopolysaccharide;Chlamydia trachomatis LPS
Immunogen
Formalin-killed elementary bodies of the Chlamydia trachomatis L2 serovar.
Quantity
25 μg
Research Discipline
Inflammation & Immunology
Test Specificity
Clone EVH-H1 detects lipopolysaccharide from Chlamydia genus.
Content And Storage
Stable for 1 year at 2-8°C from date of receipt.
Isotype
IgG2a
Related Products
Description
- Anti-Chlamydial LPS, clone EVI-H1, Cat
- No
- MABF2107, is a mouse monoclonal antibody that detects Chlamydial lipopolysaccharide (LPS) and has been tested for use in Electron Microscopy, Immunocytochemistry, Immunofluorescence, and Western Blotting
- Chlamydia trachomatis is a Gram-negative bacterium that is responsible to sexually transmitted diseases leading to pelvic inflammatory disease, ectopic pregnancy, infertility, and outbreaks of trachoma-associated blindness and lymphogranuloma venereum (LGV)
- Chlamydia trachomatis consists of eighteen different serological variants (serovars) that include a few subvariants
- These are identified based on serological reactivity of the epitopes on their outer membrane
- Intracellularly chlamydia replicates within a vacuole
- Chlamydia infection is initiated with the expression of a chlamydial early gene product(s), which isolate the inclusion from the endocytic-lysosomal pathway and makes it fusogenic with sphingomyelin-containing exocytic vesicles
- This change in vesicular interaction allows the delivery of the vacuole to the peri-Golgi region of the host cell
- Antigens from all members of the Chlamydia genus display heat resistance and sensitivity to oxidation by sodium periodate
- Lipopolysaccharides (LPS) of Chlamydia consists of at least three antigen domains, two of which are shared by the LPS of certain other gram-negative organisms and one that is unique only to chlamydial LPS
- It has been reported that the quantitative yields of chlamydial LPS can be achieved when elementary bodies (EBs) are first reduced and alkylated prior to extraction with hot phenol-water
- (Ref.: Caldwell, HD., and Hitchcock, PJ
- (1984)
- Infect
- Immun
- (44(2); 306-314; Scidmore, MA., et al
- (1996)
- Infect
- Immun
- 64(12); 5366-5372; Turingan, RS et al
- (2017)
- PLoS ONE 12(5): e0178653).
Compare Similar Items
Show Difference
Antigen: Chlamydial LPS
Classification: Monoclonal
Conjugate: Unconjugated
Formulation: Purified mouse monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Host Species: Mouse
Purification Method: Protein G purified
Regulatory Status: RUO
Primary or Secondary: Primary
Target Species: Bacteria
Form: Purified
Applications: Immunocytochemistry, Immunofluorescence, Immunomicroscopy, Western Blot
Clone: EVI-H1
Dilution: Immunofluorescence Analysis: A representative lot detected Chlamydial LPS in Immunofluorescence applications (Su, H., et. al. (2000). Infect Immun. 68(1):192-6; Wolf, K., et. al. (2001). Infect Immun. 69(5):3082-91).Immunocytochemistry Analysis: A representative lot detected Chlamydial LPS in Immunocytochemistry applications (Wolf, K., et. al. (2001). Infect Immun. 69(5):3082-91).Electron Microscopy Analysis: A representative lot detected Chlamydial LPS with Trasnsmission Electron Microscopy applications (Wolf, K., et. al. (2001). Infect Immun. 69(5):3082-91).
Gene Alias: Chlamydial lipopolysaccharide;Chlamydia trachomatis LPS
Immunogen: Formalin-killed elementary bodies of the Chlamydia trachomatis L2 serovar.
Quantity: 25 μg
Research Discipline: Inflammation & Immunology
Test Specificity: Clone EVH-H1 detects lipopolysaccharide from Chlamydia genus.
Content And Storage: Stable for 1 year at 2-8°C from date of receipt.
Isotype: IgG2a
Antigen:
Chlamydial LPS
Classification:
Monoclonal
Conjugate:
Unconjugated
Formulation:
Purified mouse monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Host Species:
Mouse
Purification Method:
Protein G purified
Regulatory Status:
RUO
Primary or Secondary:
Primary
Target Species:
Bacteria
Form:
Purified
Applications:
Immunocytochemistry, Immunofluorescence, Immunomicroscopy, Western Blot
Clone:
EVI-H1
Dilution:
Immunofluorescence Analysis: A representative lot detected Chlamydial LPS in Immunofluorescence applications (Su, H., et. al. (2000). Infect Immun. 68(1):192-6; Wolf, K., et. al. (2001). Infect Immun. 69(5):3082-91).Immunocytochemistry Analysis: A representative lot detected Chlamydial LPS in Immunocytochemistry applications (Wolf, K., et. al. (2001). Infect Immun. 69(5):3082-91).Electron Microscopy Analysis: A representative lot detected Chlamydial LPS with Trasnsmission Electron Microscopy applications (Wolf, K., et. al. (2001). Infect Immun. 69(5):3082-91).
Gene Alias:
Chlamydial lipopolysaccharide;Chlamydia trachomatis LPS
Immunogen:
Formalin-killed elementary bodies of the Chlamydia trachomatis L2 serovar.
Quantity:
25 μg
Research Discipline:
Inflammation & Immunology
Test Specificity:
Clone EVH-H1 detects lipopolysaccharide from Chlamydia genus.
Content And Storage:
Stable for 1 year at 2-8°C from date of receipt.
Isotype:
IgG2a
Antigen: Chlamydial HSP60
Classification: Monoclonal
Conjugate: Unconjugated
Formulation: Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Host Species: Mouse
Purification Method: Protein G purified
Regulatory Status: RUO
Primary or Secondary: Primary
Target Species: Bacteria
Form: Purified
Applications: ELISA, Immunocytochemistry, Immunofluorescence, Western Blot
Clone: A57-B9
Dilution: Immunocytochemistry Analysis: A representative lot detected Chlamydial HSP60 in Immunocytochemistry applications (Yuan, Y., et. al. (1992). Infect Immun. 60(6):2288-96).Immunofluorescence Analysis: A representative lot detected Chlamydial HSP60 in Immunofluorescence applications (Southern, T., et. al. (2012). Clin Vaccine Immunol. 19(11):1864-9).ELISA Analysis: A representative lot detected Chlamydial HSP60 in ELISA applications (Morrison, S.G., et. al. (2005). J Immunol. 175(11):7536-42).Western Blotting Analysis: A representative lot detected chlamydial HSP60 in Western Blotting applications (Hechard, C., et. al. (2004). J Med Microbiol. 53(Pt 9):861-8; LaVerda, D., et. al. (1997). J Clin Microbiol. 35(5):1209-15; Southern, T., et. al. (2012). Clin Vaccine Immunol. 19(11):1864-9; Yuan, Y., et. al. (1992). Infect Immun. 60(6):2288-96).
Gene Alias: Chlamydia heat shock protein 60
Immunogen: Recombinant Chlamydia trachomatis serovar A HSP60 emulsified in complete Freund adjuvant.
Quantity: 100 μg
Research Discipline: Inflammation & Immunology
Test Specificity: Clone A57-B9 detects Chlamydial HSP60. It targets an epitope with in 144 amino acids in the carboxy terminal of HSP60.
Content And Storage: Stable for 1 year at 2-8°C from date of receipt.
Isotype: IgG1 κ
Antigen:
Chlamydial HSP60
Classification:
Monoclonal
Conjugate:
Unconjugated
Formulation:
Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Host Species:
Mouse
Purification Method:
Protein G purified
Regulatory Status:
RUO
Primary or Secondary:
Primary
Target Species:
Bacteria
Form:
Purified
Applications:
ELISA, Immunocytochemistry, Immunofluorescence, Western Blot
Clone:
A57-B9
Dilution:
Immunocytochemistry Analysis: A representative lot detected Chlamydial HSP60 in Immunocytochemistry applications (Yuan, Y., et. al. (1992). Infect Immun. 60(6):2288-96).Immunofluorescence Analysis: A representative lot detected Chlamydial HSP60 in Immunofluorescence applications (Southern, T., et. al. (2012). Clin Vaccine Immunol. 19(11):1864-9).ELISA Analysis: A representative lot detected Chlamydial HSP60 in ELISA applications (Morrison, S.G., et. al. (2005). J Immunol. 175(11):7536-42).Western Blotting Analysis: A representative lot detected chlamydial HSP60 in Western Blotting applications (Hechard, C., et. al. (2004). J Med Microbiol. 53(Pt 9):861-8; LaVerda, D., et. al. (1997). J Clin Microbiol. 35(5):1209-15; Southern, T., et. al. (2012). Clin Vaccine Immunol. 19(11):1864-9; Yuan, Y., et. al. (1992). Infect Immun. 60(6):2288-96).
Gene Alias:
Chlamydia heat shock protein 60
Immunogen:
Recombinant Chlamydia trachomatis serovar A HSP60 emulsified in complete Freund adjuvant.
Quantity:
100 μg
Research Discipline:
Inflammation & Immunology
Test Specificity:
Clone A57-B9 detects Chlamydial HSP60. It targets an epitope with in 144 amino acids in the carboxy terminal of HSP60.
Content And Storage:
Stable for 1 year at 2-8°C from date of receipt.
Isotype:
IgG1 κ
Antigen: Chlamydial HSP60
Classification: Monoclonal
Conjugate: Unconjugated
Formulation: Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Host Species: Mouse
Purification Method: Protein G purified
Regulatory Status: RUO
Primary or Secondary: Primary
Target Species: Bacteria
Form: Purified
Applications: ELISA, Immunocytochemistry, Immunofluorescence, Western Blot
Clone: A57-B9
Dilution: Immunocytochemistry Analysis: A representative lot detected Chlamydial HSP60 in Immunocytochemistry applications (Yuan, Y., et. al. (1992). Infect Immun. 60(6):2288-96).Immunofluorescence Analysis: A representative lot detected Chlamydial HSP60 in Immunofluorescence applications (Southern, T., et. al. (2012). Clin Vaccine Immunol. 19(11):1864-9).ELISA Analysis: A representative lot detected Chlamydial HSP60 in ELISA applications (Morrison, S.G., et. al. (2005). J Immunol. 175(11):7536-42).Western Blotting Analysis: A representative lot detected chlamydial HSP60 in Western Blotting applications (Hechard, C., et. al. (2004). J Med Microbiol. 53(Pt 9):861-8; LaVerda, D., et. al. (1997). J Clin Microbiol. 35(5):1209-15; Southern, T., et. al. (2012). Clin Vaccine Immunol. 19(11):1864-9; Yuan, Y., et. al. (1992). Infect Immun. 60(6):2288-96).
Gene Alias: Chlamydia heat shock protein 60
Immunogen: Recombinant Chlamydia trachomatis serovar A HSP60 emulsified in complete Freund adjuvant.
Quantity: 25 μg
Research Discipline: Inflammation & Immunology
Test Specificity: Clone A57-B9 detects Chlamydial HSP60. It targets an epitope with in 144 amino acids in the carboxy terminal of HSP60.
Content And Storage: Stable for 1 year at 2-8°C from date of receipt.
Isotype: IgG1 κ
Antigen:
Chlamydial HSP60
Classification:
Monoclonal
Conjugate:
Unconjugated
Formulation:
Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Host Species:
Mouse
Purification Method:
Protein G purified
Regulatory Status:
RUO
Primary or Secondary:
Primary
Target Species:
Bacteria
Form:
Purified
Applications:
ELISA, Immunocytochemistry, Immunofluorescence, Western Blot
Clone:
A57-B9
Dilution:
Immunocytochemistry Analysis: A representative lot detected Chlamydial HSP60 in Immunocytochemistry applications (Yuan, Y., et. al. (1992). Infect Immun. 60(6):2288-96).Immunofluorescence Analysis: A representative lot detected Chlamydial HSP60 in Immunofluorescence applications (Southern, T., et. al. (2012). Clin Vaccine Immunol. 19(11):1864-9).ELISA Analysis: A representative lot detected Chlamydial HSP60 in ELISA applications (Morrison, S.G., et. al. (2005). J Immunol. 175(11):7536-42).Western Blotting Analysis: A representative lot detected chlamydial HSP60 in Western Blotting applications (Hechard, C., et. al. (2004). J Med Microbiol. 53(Pt 9):861-8; LaVerda, D., et. al. (1997). J Clin Microbiol. 35(5):1209-15; Southern, T., et. al. (2012). Clin Vaccine Immunol. 19(11):1864-9; Yuan, Y., et. al. (1992). Infect Immun. 60(6):2288-96).
Gene Alias:
Chlamydia heat shock protein 60
Immunogen:
Recombinant Chlamydia trachomatis serovar A HSP60 emulsified in complete Freund adjuvant.
Quantity:
25 μg
Research Discipline:
Inflammation & Immunology
Test Specificity:
Clone A57-B9 detects Chlamydial HSP60. It targets an epitope with in 144 amino acids in the carboxy terminal of HSP60.
Content And Storage:
Stable for 1 year at 2-8°C from date of receipt.
Isotype:
IgG1 κ
Antigen: NKR-P1B (CD161b)
Classification: Monoclonal
Conjugate: Unconjugated
Formulation: __
Host Species: Mouse
Purification Method: Protein G Purified
Regulatory Status: __
Primary or Secondary: Primary
Target Species: Mouse
Form: Purified
Applications: Flow Cytometry, Functional Assay, Immunoprecipitation, Western Blot
Clone: 2D12
Dilution: __
Gene Alias: Killer cell lectin-like receptor subfamily B member 1B allele B;CD161 antigen-like family member B;Inhibitory receptor NKR-P1B;Lymphocyte antigen 55d;Ly-55d;Natural killer cell surface protein NKR-P1B allele B6;CD161b
Immunogen: Mouse BaF/3 cells (murine IL-3-dependent pro-B cell line) expressing mouse NKR-P1B protein.
Quantity: 100 μL
Research Discipline: Inflammation and Immunology
Test Specificity: Clone 2D12 is a mouse monoclonal antibody that detects Natural killer cell surface protein NKR-P1B allele B6 (NKR-P1B) in murine species. It does not react with Nkrp1a, Nkrp1c, and Nkrp1f.
Content And Storage: Stable for 1 year at -20°C from date of receipt.
Isotype: IgG2a λ
Antigen:
NKR-P1B (CD161b)
Classification:
Monoclonal
Conjugate:
Unconjugated
Formulation:
__
Host Species:
Mouse
Purification Method:
Protein G Purified
Regulatory Status:
__
Primary or Secondary:
Primary
Target Species:
Mouse
Form:
Purified
Applications:
Flow Cytometry, Functional Assay, Immunoprecipitation, Western Blot
Clone:
2D12
Dilution:
__
Gene Alias:
Killer cell lectin-like receptor subfamily B member 1B allele B;CD161 antigen-like family member B;Inhibitory receptor NKR-P1B;Lymphocyte antigen 55d;Ly-55d;Natural killer cell surface protein NKR-P1B allele B6;CD161b
Immunogen:
Mouse BaF/3 cells (murine IL-3-dependent pro-B cell line) expressing mouse NKR-P1B protein.
Quantity:
100 μL
Research Discipline:
Inflammation and Immunology
Test Specificity:
Clone 2D12 is a mouse monoclonal antibody that detects Natural killer cell surface protein NKR-P1B allele B6 (NKR-P1B) in murine species. It does not react with Nkrp1a, Nkrp1c, and Nkrp1f.
Content And Storage:
Stable for 1 year at -20°C from date of receipt.
Isotype:
IgG2a λ