MABS190725
Ketohexokinase Isoform C, Mouse anti-Human, Clone: 5F1-3F5, Millipore Sigma™
Manufacturer: Fischer Scientific
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| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | MABS190725-Each-of-1 | In Stock | ₹ 15,999.72 |
MABS190725 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 15,999.72
GST (18%): ₹ 2,879.95
Total Price: ₹ 18,879.67
Antigen
Ketohexokinase Isoform C
Classification
Monoclonal
Conjugate
Unconjugated
Formulation
Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Gene Symbols
KHK
Immunogen
A full-length human recombinant Ketohexokinase, isoform C.
Quantity
25 μg
Research Discipline
Signaling
Gene ID (Entrez)
NP_000212
Target Species
Human
Form
Purified
Applications
Western Blot
Clone
5F1-3F5
Dilution
Western Blotting Analysis: 2μg/mL from a represenative lot detected Ketohexokinase Isoform C in HEK293 cells expressing HA-tagged KHK-C.Western Blotting Analysis: 1μg/mL from a represenative lot detected Ketohexokinase Isoform C in human liver tissue lysate.
Gene Alias
EC: 2.7.1.3;Hepatic fructokinase;Fructokinase
Host Species
Mouse
Purification Method
Protein G purified
Regulatory Status
RUO
Primary or Secondary
Primary
Test Specificity
Clone 5F1-3F5 specifically detects isoform C of human ketohexokinase.
Content And Storage
Stable for 1 year at 2-8°C from date of receipt.
Isotype
IgG1 κ
Related Products
Description
- Anti-Ketohexokinase Isoform C, clone 5F1-3F5, Cat
- No
- MABS1907, is a mouse monoclonal antibody that detectsKetohexokinase and has been tested for use in Western Blotting
- Ketohexokinase (UniProt: P50053; also known as EC: 2.7.1.3, Hepatic fructokinase) is encoded by the KHK gene (Gene ID: 3795) in human
- Ketohexokinase is a homodimeric cytosolic enzyme in that catalyzes the conversion of fructose to fructose 1 phosphate
- It is most abundant in the liver, where it may constitute up to 0.6% of the total liver protein
- It has also been reported in kidney, spleen, gut, and pancreas
- Very low levels are also found in adrenal, muscle, brain, and eyes
- It has multiple fructose binding sites
- There are two major isoforms of this enzyme ketofructokinase C and ketofructokinase A
- Due to its high Km (∽ 28 mM), fructose is preferentially metabolized by the C isoform
- Ketohexokinase -C is unique among sugar kinases in that its metabolism of fructose is associated with a rapid depletion of intracellular ATP
- Unlike glucokinase, in which excessive phosphorylation of glucose is prevented by a negative feedback system, the metabolism of fructose by ketohexokinase-C results in rapid phosphorylation with a fall in intracellular phosphate and ATP
- If the ATP levels fall significantly then the protein synthesis is transiently stopped and cells may develop features that are consistent with ischemia
- Mutations in KHK gene has been linked to fructosuria, a clinically benign condition that is characterized nu the incomplete metabolism of fructose in the liver, leading to its urinary excretion
- (Ref.: Diggle, CP et al., (2009)
- J
- Histochem
- Cytochem
- 57(8): 763-774; Hayward, BE., and Bonthron, DT (1998)
- Eur
- J
- Biochem
- 257(1); 85-91).
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Antigen: Ketohexokinase Isoform C
Classification: Monoclonal
Conjugate: Unconjugated
Formulation: Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Gene Symbols: KHK
Immunogen: A full-length human recombinant Ketohexokinase, isoform C.
Quantity: 25 μg
Research Discipline: Signaling
Gene ID (Entrez): NP_000212
Target Species: Human
Form: Purified
Applications: Western Blot
Clone: 5F1-3F5
Dilution: Western Blotting Analysis: 2μg/mL from a represenative lot detected Ketohexokinase Isoform C in HEK293 cells expressing HA-tagged KHK-C.Western Blotting Analysis: 1μg/mL from a represenative lot detected Ketohexokinase Isoform C in human liver tissue lysate.
Gene Alias: EC: 2.7.1.3;Hepatic fructokinase;Fructokinase
Host Species: Mouse
Purification Method: Protein G purified
Regulatory Status: RUO
Primary or Secondary: Primary
Test Specificity: Clone 5F1-3F5 specifically detects isoform C of human ketohexokinase.
Content And Storage: Stable for 1 year at 2-8°C from date of receipt.
Isotype: IgG1 κ
Antigen:
Ketohexokinase Isoform C
Classification:
Monoclonal
Conjugate:
Unconjugated
Formulation:
Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Gene Symbols:
KHK
Immunogen:
A full-length human recombinant Ketohexokinase, isoform C.
Quantity:
25 μg
Research Discipline:
Signaling
Gene ID (Entrez):
NP_000212
Target Species:
Human
Form:
Purified
Applications:
Western Blot
Clone:
5F1-3F5
Dilution:
Western Blotting Analysis: 2μg/mL from a represenative lot detected Ketohexokinase Isoform C in HEK293 cells expressing HA-tagged KHK-C.Western Blotting Analysis: 1μg/mL from a represenative lot detected Ketohexokinase Isoform C in human liver tissue lysate.
Gene Alias:
EC: 2.7.1.3;Hepatic fructokinase;Fructokinase
Host Species:
Mouse
Purification Method:
Protein G purified
Regulatory Status:
RUO
Primary or Secondary:
Primary
Test Specificity:
Clone 5F1-3F5 specifically detects isoform C of human ketohexokinase.
Content And Storage:
Stable for 1 year at 2-8°C from date of receipt.
Isotype:
IgG1 κ
Antigen: Signal peptide peptidase-like 3
Classification: Monoclonal
Conjugate: Unconjugated
Formulation: Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Gene Symbols: SPPL3;IMP2
Immunogen: KLH-conjugated linear peptide corresponding to 15 amino acids from the C-terminal half of human Signal peptide peptidase-like 3.
Quantity: 25 μg
Research Discipline: Signaling
Gene ID (Entrez): __
Target Species: Human
Form: Purified
Applications: Immunoprecipitation, Western Blot
Clone: 7F9
Dilution: Western Blotting Analysis: A representative lot detected Signal peptide peptidase-like 3 in Western Blotting applications (Voss, M., et. al. (2012). J Biol Chem. 287(52):43401-9; Voss, M., et. al. (2014). EMBO J. 33(24):2890-905; Fleck, D., et. al. (2016). J Biol Chem. 291(1):318-33).Immunoprecipitation Analysis: A representative lot detected Signal peptide peptidase-like 3 in Immunoprecipitation applications (Voss, M., et. al. (2012). J Biol Chem. 287(52):43401-9).
Gene Alias: Signal peptide peptidase-like 3;SPP-like 3;Intramembrane protease 2;IMP-2;Presenilin homologous protein 1;PSH1;Presenilin-like protein 4
Host Species: Mouse
Purification Method: Protein G purified
Regulatory Status: RUO
Primary or Secondary: Primary
Test Specificity: Clone 7F9 detects Signal peptide peptidase-like 3 in human cells. it targets an epitope within 15 amino acids from the C-terminal half. Immunogen sequence is conserved in all three isoforms.
Content And Storage: Stable for 1 year at 2-8°C from date of receipt.
Isotype: IgG1
Antigen:
Signal peptide peptidase-like 3
Classification:
Monoclonal
Conjugate:
Unconjugated
Formulation:
Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Gene Symbols:
SPPL3;IMP2
Immunogen:
KLH-conjugated linear peptide corresponding to 15 amino acids from the C-terminal half of human Signal peptide peptidase-like 3.
Quantity:
25 μg
Research Discipline:
Signaling
Gene ID (Entrez):
__
Target Species:
Human
Form:
Purified
Applications:
Immunoprecipitation, Western Blot
Clone:
7F9
Dilution:
Western Blotting Analysis: A representative lot detected Signal peptide peptidase-like 3 in Western Blotting applications (Voss, M., et. al. (2012). J Biol Chem. 287(52):43401-9; Voss, M., et. al. (2014). EMBO J. 33(24):2890-905; Fleck, D., et. al. (2016). J Biol Chem. 291(1):318-33).Immunoprecipitation Analysis: A representative lot detected Signal peptide peptidase-like 3 in Immunoprecipitation applications (Voss, M., et. al. (2012). J Biol Chem. 287(52):43401-9).
Gene Alias:
Signal peptide peptidase-like 3;SPP-like 3;Intramembrane protease 2;IMP-2;Presenilin homologous protein 1;PSH1;Presenilin-like protein 4
Host Species:
Mouse
Purification Method:
Protein G purified
Regulatory Status:
RUO
Primary or Secondary:
Primary
Test Specificity:
Clone 7F9 detects Signal peptide peptidase-like 3 in human cells. it targets an epitope within 15 amino acids from the C-terminal half. Immunogen sequence is conserved in all three isoforms.
Content And Storage:
Stable for 1 year at 2-8°C from date of receipt.
Isotype:
IgG1
Antigen: Mitochondrial Pyruvate Carrier 2 (BRP44)
Classification: Monoclonal
Conjugate: Unconjugated
Formulation: Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Gene Symbols: MCP2;BRP44
Immunogen: A linear peptide corresponding to 10 amino acids from the internal region of human mitochondrial pyruvate carrier 2 protein.
Quantity: 25 μg
Research Discipline: Signaling
Gene ID (Entrez): __
Target Species: Human, Mouse
Form: Purified
Applications: Immunoprecipitation, Western Blot
Clone: __
Dilution: Western Blotting Analysis: 1μg/mL from a representative lot detected Mitochondrial Pyruvate Carrier 2 (BRP44) in mouse heart and mouse liver tissue lysates. Immunoprecipitation Analysis: A 1:1,000 dilution from a representative lot immunoprecipitated Mitochondrial Pyruvate Carrier 2 (BRP44) in mouse liver mitoplasts (Courtesy of Prof. Jean-Claude Martinou, Ph.D., University of Geneva, Switzerland).. Western Blotting Analysis: 4 μg/mL from a represenetative lot detected Mitochondrial Pyruvate Carrier 2 (BRP44) in mouse pancreas tissue lysate. Western Blotting Analysis: A representative lot detected Mitochondrial Pyruvate Carrier 2 (BRP44) in Western Blotting applications (Compan, V., et. al. (2015). Mol Cell. 59(3):491-501; Vanderperre, B., et. al. (2016). PLoS Genet. 12(5):e1006056).
Gene Alias: Mitochondrial pyruvate carrier 2;Brain protein 44;BRP44
Host Species: Mouse
Purification Method: Protein G purified
Regulatory Status: RUO
Primary or Secondary: Primary
Test Specificity: This mouse monoclonal antibody detects Mitochondrial Pyruvate carrrier Protein 2 in human and mouse cells. It targets an epitope within 10 amino acids from the internal region.
Content And Storage: Stable for 1 year at 2-8°C from date of receipt.
Isotype: IgG1
Antigen:
Mitochondrial Pyruvate Carrier 2 (BRP44)
Classification:
Monoclonal
Conjugate:
Unconjugated
Formulation:
Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Gene Symbols:
MCP2;BRP44
Immunogen:
A linear peptide corresponding to 10 amino acids from the internal region of human mitochondrial pyruvate carrier 2 protein.
Quantity:
25 μg
Research Discipline:
Signaling
Gene ID (Entrez):
__
Target Species:
Human, Mouse
Form:
Purified
Applications:
Immunoprecipitation, Western Blot
Clone:
__
Dilution:
Western Blotting Analysis: 1μg/mL from a representative lot detected Mitochondrial Pyruvate Carrier 2 (BRP44) in mouse heart and mouse liver tissue lysates. Immunoprecipitation Analysis: A 1:1,000 dilution from a representative lot immunoprecipitated Mitochondrial Pyruvate Carrier 2 (BRP44) in mouse liver mitoplasts (Courtesy of Prof. Jean-Claude Martinou, Ph.D., University of Geneva, Switzerland).. Western Blotting Analysis: 4 μg/mL from a represenetative lot detected Mitochondrial Pyruvate Carrier 2 (BRP44) in mouse pancreas tissue lysate. Western Blotting Analysis: A representative lot detected Mitochondrial Pyruvate Carrier 2 (BRP44) in Western Blotting applications (Compan, V., et. al. (2015). Mol Cell. 59(3):491-501; Vanderperre, B., et. al. (2016). PLoS Genet. 12(5):e1006056).
Gene Alias:
Mitochondrial pyruvate carrier 2;Brain protein 44;BRP44
Host Species:
Mouse
Purification Method:
Protein G purified
Regulatory Status:
RUO
Primary or Secondary:
Primary
Test Specificity:
This mouse monoclonal antibody detects Mitochondrial Pyruvate carrrier Protein 2 in human and mouse cells. It targets an epitope within 10 amino acids from the internal region.
Content And Storage:
Stable for 1 year at 2-8°C from date of receipt.
Isotype:
IgG1
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