MABS2034100
SREBP-1, Mouse anti-Hamster, Canine, Human, Clone: 2A4, Millipore Sigma™
Manufacturer: Fischer Scientific
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | MABS2034100-Each-of-1 | In Stock | ₹ 41,308.37 |
MABS2034100 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 41,308.37
GST (18%): ₹ 7,435.507
Total Price: ₹ 48,743.877
Antigen
SREBP-1
Classification
Monoclonal
Conjugate
Unconjugated
Formulation
Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Gene Symbols
SREBF1;BHLHD1;SREBP1
Immunogen
His-tagged recombinant fragment corresponding to 107 amino acids from the N-terminal half of human Sterol regulatory element-binding protein 1 (SREBP-1).
Quantity
100 μg
Research Discipline
Signaling
Gene ID (Entrez)
NP_001005291.1
Target Species
Hamster, Canine, Human
Form
Purified
Applications
Western Blot
Clone
2A4
Dilution
Western Blotting Analysis: A representative lot detected SREBP-1 in Western Blotting applications (Toth, J.I., et. al. (2004). Mol Cell Biol. 24(18):8288-300; Key, C.C., et. al. (2017). Cell Rep. 19(10):2116-2129; Rosenfeld, J.M., et. al. (1998). J Biol Chem. 273(26):16112-21; Sato, R., et. al. (1994). J Biol Chem. 269(25):17267-73).
Gene Alias
Sterol regulatory element-binding protein 1;Class D basic helix-loop-helix protein 1;bHLHd1;Sterol regulatory element-binding transcription factor 1
Host Species
Mouse
Purification Method
Protein G purified
Regulatory Status
RUO
Primary or Secondary
Primary
Test Specificity
Clone 2A4 is a mouse monoclonal antibody that detects Sterol regulatory element-binding protein 1. It targets an epitope within 107 amino acids from the N-terminal half.
Content And Storage
Stable for 1 year at 2-8°C from date of receipt.
Isotype
IgG1 κ
Related Products
Description
- Anti-SREBP-1, clone 2A4, Cat
- No
- MABS2034, is a mouse monoclonal antibody that detects Sterol regulatory element-binding protein 1 and has been tested for use in Western Blotting
- Sterol regulatory element-binding protein 1 (UniProt: P36956; also known as SREBP-1, Class D basic helix-loop-helix protein 1, bHLHd1, Sterol regulatory element-binding transcription factor 1) is encoded by the SREBF1 (also known as BHLHD1, SREBP1) gene (Gene ID: 6720) in human
- SREBP-1 is a multi-pass membrane protein found on nuclear, endoplasmic reticulum, and Golgi membranes
- It attaches to membranes via two hydrophobic sequences (aa 477-497 and 536-556)
- It is expressed in a wide variety of tissues, but is most abundant in liver and adrenal gland
- Six isoforms of SREBP-1 have been described that are produced by alternative splicing
- Isoform SREBP-1C predominates in liver, adrenal gland, and ovary, whereas isoform SREBP-1A predominates in hepatoma cell lines
- Both isoforms are found in kidney, brain, white fat, and muscle
- It serves as a transcriptional activator that is essential for lipid homeostasis and regulates transcription of the LDL receptor gene
- It is also involved in fatty acid and cholesterol synthesis
- It acts by binds to the sterol regulatory element 1 (SRE-1) (5'-ATCACCCCAC-3') and displays dual sequence specificity binding to both an E-box motif (5'-ATCACGTGA-3') and to SRE-1 (5'-ATCACCCCAC-3')
- SREBP-1 has two cytoplasmic domains (aa 1-487 and 569-1147), two transmembrane domains (aa 88-508 and 548-568), and one lumenal domain (aa 509-547)
- In the precursor form, which has a hairpin orientation in the membrane both the N-terminal transcription factor domain and the C-terminal regulatory domain face the cytoplasm
- Two separate site-specific proteolytic cleavages are required for release of the transcriptionally active N-terminal domain
- These cleavages are carried out by two distinct proteases, called site-1 protease (S1P; aa 490-491) and site-2 protease (S2P; aa 530-531)
- SREBP-1 can be phosphorylated by AMPK at Ser402, which suppress its processing and nuclear translocation, and thereby repress target gene expression.
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Antigen: SREBP-1
Classification: Monoclonal
Conjugate: Unconjugated
Formulation: Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Gene Symbols: SREBF1;BHLHD1;SREBP1
Immunogen: His-tagged recombinant fragment corresponding to 107 amino acids from the N-terminal half of human Sterol regulatory element-binding protein 1 (SREBP-1).
Quantity: 100 μg
Research Discipline: Signaling
Gene ID (Entrez): NP_001005291.1
Target Species: Hamster, Canine, Human
Form: Purified
Applications: Western Blot
Clone: 2A4
Dilution: Western Blotting Analysis: A representative lot detected SREBP-1 in Western Blotting applications (Toth, J.I., et. al. (2004). Mol Cell Biol. 24(18):8288-300; Key, C.C., et. al. (2017). Cell Rep. 19(10):2116-2129; Rosenfeld, J.M., et. al. (1998). J Biol Chem. 273(26):16112-21; Sato, R., et. al. (1994). J Biol Chem. 269(25):17267-73).
Gene Alias: Sterol regulatory element-binding protein 1;Class D basic helix-loop-helix protein 1;bHLHd1;Sterol regulatory element-binding transcription factor 1
Host Species: Mouse
Purification Method: Protein G purified
Regulatory Status: RUO
Primary or Secondary: Primary
Test Specificity: Clone 2A4 is a mouse monoclonal antibody that detects Sterol regulatory element-binding protein 1. It targets an epitope within 107 amino acids from the N-terminal half.
Content And Storage: Stable for 1 year at 2-8°C from date of receipt.
Isotype: IgG1 κ
Antigen:
SREBP-1
Classification:
Monoclonal
Conjugate:
Unconjugated
Formulation:
Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Gene Symbols:
SREBF1;BHLHD1;SREBP1
Immunogen:
His-tagged recombinant fragment corresponding to 107 amino acids from the N-terminal half of human Sterol regulatory element-binding protein 1 (SREBP-1).
Quantity:
100 μg
Research Discipline:
Signaling
Gene ID (Entrez):
NP_001005291.1
Target Species:
Hamster, Canine, Human
Form:
Purified
Applications:
Western Blot
Clone:
2A4
Dilution:
Western Blotting Analysis: A representative lot detected SREBP-1 in Western Blotting applications (Toth, J.I., et. al. (2004). Mol Cell Biol. 24(18):8288-300; Key, C.C., et. al. (2017). Cell Rep. 19(10):2116-2129; Rosenfeld, J.M., et. al. (1998). J Biol Chem. 273(26):16112-21; Sato, R., et. al. (1994). J Biol Chem. 269(25):17267-73).
Gene Alias:
Sterol regulatory element-binding protein 1;Class D basic helix-loop-helix protein 1;bHLHd1;Sterol regulatory element-binding transcription factor 1
Host Species:
Mouse
Purification Method:
Protein G purified
Regulatory Status:
RUO
Primary or Secondary:
Primary
Test Specificity:
Clone 2A4 is a mouse monoclonal antibody that detects Sterol regulatory element-binding protein 1. It targets an epitope within 107 amino acids from the N-terminal half.
Content And Storage:
Stable for 1 year at 2-8°C from date of receipt.
Isotype:
IgG1 κ
Antigen: SREBP-1
Classification: Monoclonal
Conjugate: Unconjugated
Formulation: Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Gene Symbols: SREBF1;BHLHD1;SREBP1
Immunogen: His-tagged recombinant fragment corresponding to 107 amino acids from the N-terminal half of human Sterol regulatory element-binding protein 1 (SREBP-1).
Quantity: 25 μg
Research Discipline: Signaling
Gene ID (Entrez): NP_001005291.1
Target Species: Hamster, Canine, Human
Form: Purified
Applications: Western Blot
Clone: 2A4
Dilution: Western Blotting Analysis: A representative lot detected SREBP-1 in Western Blotting applications (Toth, J.I., et. al. (2004). Mol Cell Biol. 24(18):8288-300; Key, C.C., et. al. (2017). Cell Rep. 19(10):2116-2129; Rosenfeld, J.M., et. al. (1998). J Biol Chem. 273(26):16112-21; Sato, R., et. al. (1994). J Biol Chem. 269(25):17267-73).
Gene Alias: Sterol regulatory element-binding protein 1;Class D basic helix-loop-helix protein 1;bHLHd1;Sterol regulatory element-binding transcription factor 1
Host Species: Mouse
Purification Method: Protein G purified
Regulatory Status: RUO
Primary or Secondary: Primary
Test Specificity: Clone 2A4 is a mouse monoclonal antibody that detects Sterol regulatory element-binding protein 1. It targets an epitope within 107 amino acids from the N-terminal half.
Content And Storage: Stable for 1 year at 2-8°C from date of receipt.
Isotype: IgG1 κ
Antigen:
SREBP-1
Classification:
Monoclonal
Conjugate:
Unconjugated
Formulation:
Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Gene Symbols:
SREBF1;BHLHD1;SREBP1
Immunogen:
His-tagged recombinant fragment corresponding to 107 amino acids from the N-terminal half of human Sterol regulatory element-binding protein 1 (SREBP-1).
Quantity:
25 μg
Research Discipline:
Signaling
Gene ID (Entrez):
NP_001005291.1
Target Species:
Hamster, Canine, Human
Form:
Purified
Applications:
Western Blot
Clone:
2A4
Dilution:
Western Blotting Analysis: A representative lot detected SREBP-1 in Western Blotting applications (Toth, J.I., et. al. (2004). Mol Cell Biol. 24(18):8288-300; Key, C.C., et. al. (2017). Cell Rep. 19(10):2116-2129; Rosenfeld, J.M., et. al. (1998). J Biol Chem. 273(26):16112-21; Sato, R., et. al. (1994). J Biol Chem. 269(25):17267-73).
Gene Alias:
Sterol regulatory element-binding protein 1;Class D basic helix-loop-helix protein 1;bHLHd1;Sterol regulatory element-binding transcription factor 1
Host Species:
Mouse
Purification Method:
Protein G purified
Regulatory Status:
RUO
Primary or Secondary:
Primary
Test Specificity:
Clone 2A4 is a mouse monoclonal antibody that detects Sterol regulatory element-binding protein 1. It targets an epitope within 107 amino acids from the N-terminal half.
Content And Storage:
Stable for 1 year at 2-8°C from date of receipt.
Isotype:
IgG1 κ
Antigen: Factor XII
Classification: Monoclonal
Conjugate: Unconjugated
Formulation: __
Gene Symbols: F12
Immunogen: A mixture of purified human Factor XII and Factor XIIa
Quantity: 100 μg
Research Discipline: Signaling
Gene ID (Entrez): NP_000496
Target Species: Baboon, Human
Form: Purified
Applications: ELISA, Inhibition Assays, Western Blot
Clone: 15H8
Dilution: __
Gene Alias: __
Host Species: Mouse
Purification Method: Protein G Purified
Regulatory Status: RUO
Primary or Secondary: Primary
Test Specificity: Clone 15H8 is a mouse monoclonal antibody that specifically detects Coagulation factor XII.
Content And Storage: Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Isotype: IgG2a κ
Antigen:
Factor XII
Classification:
Monoclonal
Conjugate:
Unconjugated
Formulation:
__
Gene Symbols:
F12
Immunogen:
A mixture of purified human Factor XII and Factor XIIa
Quantity:
100 μg
Research Discipline:
Signaling
Gene ID (Entrez):
NP_000496
Target Species:
Baboon, Human
Form:
Purified
Applications:
ELISA, Inhibition Assays, Western Blot
Clone:
15H8
Dilution:
__
Gene Alias:
__
Host Species:
Mouse
Purification Method:
Protein G Purified
Regulatory Status:
RUO
Primary or Secondary:
Primary
Test Specificity:
Clone 15H8 is a mouse monoclonal antibody that specifically detects Coagulation factor XII.
Content And Storage:
Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Isotype:
IgG2a κ
Antigen: Factor XII
Classification: Monoclonal
Conjugate: Unconjugated
Formulation: __
Gene Symbols: F12
Immunogen: A mixture of purified human Factor XII and Factor XIIa
Quantity: 25 μg
Research Discipline: Signaling
Gene ID (Entrez): NP_000496
Target Species: Baboon, Human
Form: Purified
Applications: ELISA, Inhibition Assays, Western Blot
Clone: 15H8
Dilution: __
Gene Alias: __
Host Species: Mouse
Purification Method: Protein G Purified
Regulatory Status: RUO
Primary or Secondary: Primary
Test Specificity: Clone 15H8 is a mouse monoclonal antibody that specifically detects Coagulation factor XII.
Content And Storage: Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Isotype: IgG2a κ
Antigen:
Factor XII
Classification:
Monoclonal
Conjugate:
Unconjugated
Formulation:
__
Gene Symbols:
F12
Immunogen:
A mixture of purified human Factor XII and Factor XIIa
Quantity:
25 μg
Research Discipline:
Signaling
Gene ID (Entrez):
NP_000496
Target Species:
Baboon, Human
Form:
Purified
Applications:
ELISA, Inhibition Assays, Western Blot
Clone:
15H8
Dilution:
__
Gene Alias:
__
Host Species:
Mouse
Purification Method:
Protein G Purified
Regulatory Status:
RUO
Primary or Secondary:
Primary
Test Specificity:
Clone 15H8 is a mouse monoclonal antibody that specifically detects Coagulation factor XII.
Content And Storage:
Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Isotype:
IgG2a κ