MABT1330100
ADAM 12, Mouse anti-Human, Clone: 8F8, Millipore Sigma™
Manufacturer: Fischer Scientific
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | MABT1330100-Each-of-1 | In Stock | ₹ 42,676.47 |
MABT1330100 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 42,676.47
GST (18%): ₹ 7,681.765
Total Price: ₹ 50,358.235
Antigen
ADAM 12
Classification
Monoclonal
Conjugate
Unconjugated
Formulation
Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Gene Symbols
ADAM12;MLTN;UNQ346/PRO545
Immunogen
A full-length recombinant human ADAM12 secreted expressed in HEK293 cells.
Quantity
100 μg
Research Discipline
Apoptosis & Cancer
Gene ID (Entrez)
NP_001275903
Target Species
Human
Form
Purified
Applications
Flow Cytometry, Immunocytochemistry, Immunoprecipitation, Inhibition Assays, Western Blot
Clone
8F8
Dilution
Immunoprecipitation Analysis: A representative lot immunoprecipitated ADAM 12 in Immunoprecipitation applications (Kveiborg, M., et. al. (2005). Cancer Res. 65(11):4754-61).Inhibition Analysis: A representative inhibited ADAM 12 induced gelatin degradation. (Albrechtsen, R., et. al. (2013). J Cell Sci. 126(Pt 20):4707-20). Flow Cytometry Analysis: 1μg from a representative lot detected ADAM 12 in one million ADAM 12-DeltaCyt MCF-7 cells.Immunocytochemistry Analysis: A representative lot detected ADAM 12 in Immunocytochemistry applications (Sundberg, C., et. al. (2004). J Biol Chem. 279(49):51601-11).Western Blotting Analysis: A representative lot detected ADAM 12 in Western Blotting applications (Kveiborg, M., et. al. (2005). Cancer Res. 65(11):4754-61).
Gene Alias
Disintegrin and metalloproteinase domain-containing protein 12;Meltrin-alpha
Host Species
Mouse
Purification Method
Protein G purified
Regulatory Status
RUO
Primary or Secondary
Primary
Test Specificity
Clone 8F8 detects ADAM 12-DeltaCyt secreted form in human cells. It targets an epitope with in the prodomain region.
Content And Storage
Stable for 1 year at 2-8°C from date of receipt.
Isotype
IgG1 κ
Related Products
Description
- Anti-ADAM 12, clone 8F8, Cat
- No
- MABT1330, is a mouse monoclonal antibody that detects Disintegrin and metalloproteinase domain-containing protein 12 (ADAM12) and has been tested for use in Flow Cytometry, Immunocytochemistry, Immunoprecipitation, Inhibition Assays, and Western Blotting
- Disintegrin and metalloproteinase domain-containing protein 12 (UniProt: O43184, also known as ADAM 12, Meltrin-alpha) is encoded by the ADAM12 (also known as MLTN, UNQ346/PRO545) gene (Gene ID: 8038) in human
- ADAM (A disintegrin and metalloprotease) family consists of multidomain cell-surface proteins that are synthesized in their proform
- Four isoforms of ADAM12 have been described that are produced by alternative splicing
- Isoform 1 is a single-pass transmembrane protein and isoforms 2,3, and 4 are secreted proteins
- ADM12 is synthesized with a signal peptide (aa 1-28) and a propeptide (aa 29-207), which are subsequently cleaved off in the mature form
- Isoform 1 shown to be expressed in placenta and in skeletal, cardiac, and smooth muscle
- Isoform 2 is reported to be expressed only in placenta or in embryo and fetus
- ADAM12 contains a cysteine rich domain (aa 514-649) that support cell adhesion through syndecans and triggers signaling events that lead to beta-1 integrin-dependent cell spreading
- However, in carcinoma cells the binding of this domain to syndecans does not allow the integrin-mediated cell spreading
- The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme
- Dissociation of the cysteine from the zinc ion activates the enzyme
- Upregulation of ADAM12 has been reported in malignant tumors
- In human breast cancer cells expressing ADAM12, MMP-14 is shown to be recruited to the cell surface which results in ADAM12 activation and accelerates tumor progression
- ADAM12 does not affect tumor cell proliferation, but it causes a diminution in tumor cell apoptosis and increases stromal cell apoptosis
- (Ref.: Sundberg, C., et al
- (2004)
- J
- Biol
- Chem
- 279(49); 51601 51611; Albrechtsen, R., et al
- (2013)
- J
- Cell Sci
- 126(20); 4707 4720; Kveiborg, M., et al
- (2005)
- Cancer Res
- 65(11); 4754-4761).
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Antigen: ADAM 12
Classification: Monoclonal
Conjugate: Unconjugated
Formulation: Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Gene Symbols: ADAM12;MLTN;UNQ346/PRO545
Immunogen: A full-length recombinant human ADAM12 secreted expressed in HEK293 cells.
Quantity: 100 μg
Research Discipline: Apoptosis & Cancer
Gene ID (Entrez): NP_001275903
Target Species: Human
Form: Purified
Applications: Flow Cytometry, Immunocytochemistry, Immunoprecipitation, Inhibition Assays, Western Blot
Clone: 8F8
Dilution: Immunoprecipitation Analysis: A representative lot immunoprecipitated ADAM 12 in Immunoprecipitation applications (Kveiborg, M., et. al. (2005). Cancer Res. 65(11):4754-61).Inhibition Analysis: A representative inhibited ADAM 12 induced gelatin degradation. (Albrechtsen, R., et. al. (2013). J Cell Sci. 126(Pt 20):4707-20). Flow Cytometry Analysis: 1μg from a representative lot detected ADAM 12 in one million ADAM 12-DeltaCyt MCF-7 cells.Immunocytochemistry Analysis: A representative lot detected ADAM 12 in Immunocytochemistry applications (Sundberg, C., et. al. (2004). J Biol Chem. 279(49):51601-11).Western Blotting Analysis: A representative lot detected ADAM 12 in Western Blotting applications (Kveiborg, M., et. al. (2005). Cancer Res. 65(11):4754-61).
Gene Alias: Disintegrin and metalloproteinase domain-containing protein 12;Meltrin-alpha
Host Species: Mouse
Purification Method: Protein G purified
Regulatory Status: RUO
Primary or Secondary: Primary
Test Specificity: Clone 8F8 detects ADAM 12-DeltaCyt secreted form in human cells. It targets an epitope with in the prodomain region.
Content And Storage: Stable for 1 year at 2-8°C from date of receipt.
Isotype: IgG1 κ
Antigen:
ADAM 12
Classification:
Monoclonal
Conjugate:
Unconjugated
Formulation:
Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Gene Symbols:
ADAM12;MLTN;UNQ346/PRO545
Immunogen:
A full-length recombinant human ADAM12 secreted expressed in HEK293 cells.
Quantity:
100 μg
Research Discipline:
Apoptosis & Cancer
Gene ID (Entrez):
NP_001275903
Target Species:
Human
Form:
Purified
Applications:
Flow Cytometry, Immunocytochemistry, Immunoprecipitation, Inhibition Assays, Western Blot
Clone:
8F8
Dilution:
Immunoprecipitation Analysis: A representative lot immunoprecipitated ADAM 12 in Immunoprecipitation applications (Kveiborg, M., et. al. (2005). Cancer Res. 65(11):4754-61).Inhibition Analysis: A representative inhibited ADAM 12 induced gelatin degradation. (Albrechtsen, R., et. al. (2013). J Cell Sci. 126(Pt 20):4707-20). Flow Cytometry Analysis: 1μg from a representative lot detected ADAM 12 in one million ADAM 12-DeltaCyt MCF-7 cells.Immunocytochemistry Analysis: A representative lot detected ADAM 12 in Immunocytochemistry applications (Sundberg, C., et. al. (2004). J Biol Chem. 279(49):51601-11).Western Blotting Analysis: A representative lot detected ADAM 12 in Western Blotting applications (Kveiborg, M., et. al. (2005). Cancer Res. 65(11):4754-61).
Gene Alias:
Disintegrin and metalloproteinase domain-containing protein 12;Meltrin-alpha
Host Species:
Mouse
Purification Method:
Protein G purified
Regulatory Status:
RUO
Primary or Secondary:
Primary
Test Specificity:
Clone 8F8 detects ADAM 12-DeltaCyt secreted form in human cells. It targets an epitope with in the prodomain region.
Content And Storage:
Stable for 1 year at 2-8°C from date of receipt.
Isotype:
IgG1 κ
Antigen: ADAM 12
Classification: Monoclonal
Conjugate: Unconjugated
Formulation: Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Gene Symbols: ADAM12;MLTN;UNQ346/PRO545
Immunogen: A full-length recombinant human ADAM12 secreted expressed in HEK293 cells.
Quantity: 25 μg
Research Discipline: Apoptosis & Cancer
Gene ID (Entrez): NP_001275903
Target Species: Human
Form: Purified
Applications: Flow Cytometry, Immunocytochemistry, Immunoprecipitation, Inhibition Assays, Western Blot
Clone: 8F8
Dilution: Immunoprecipitation Analysis: A representative lot immunoprecipitated ADAM 12 in Immunoprecipitation applications (Kveiborg, M., et. al. (2005). Cancer Res. 65(11):4754-61).Inhibition Analysis: A representative inhibited ADAM 12 induced gelatin degradation. (Albrechtsen, R., et. al. (2013). J Cell Sci. 126(Pt 20):4707-20). Flow Cytometry Analysis: 1μg from a representative lot detected ADAM 12 in one million ADAM 12-DeltaCyt MCF-7 cells.Immunocytochemistry Analysis: A representative lot detected ADAM 12 in Immunocytochemistry applications (Sundberg, C., et. al. (2004). J Biol Chem. 279(49):51601-11).Western Blotting Analysis: A representative lot detected ADAM 12 in Western Blotting applications (Kveiborg, M., et. al. (2005). Cancer Res. 65(11):4754-61).
Gene Alias: Disintegrin and metalloproteinase domain-containing protein 12;Meltrin-alpha
Host Species: Mouse
Purification Method: Protein G purified
Regulatory Status: RUO
Primary or Secondary: Primary
Test Specificity: Clone 8F8 detects ADAM 12-DeltaCyt secreted form in human cells. It targets an epitope with in the prodomain region.
Content And Storage: Stable for 1 year at 2-8°C from date of receipt.
Isotype: IgG1 κ
Antigen:
ADAM 12
Classification:
Monoclonal
Conjugate:
Unconjugated
Formulation:
Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Gene Symbols:
ADAM12;MLTN;UNQ346/PRO545
Immunogen:
A full-length recombinant human ADAM12 secreted expressed in HEK293 cells.
Quantity:
25 μg
Research Discipline:
Apoptosis & Cancer
Gene ID (Entrez):
NP_001275903
Target Species:
Human
Form:
Purified
Applications:
Flow Cytometry, Immunocytochemistry, Immunoprecipitation, Inhibition Assays, Western Blot
Clone:
8F8
Dilution:
Immunoprecipitation Analysis: A representative lot immunoprecipitated ADAM 12 in Immunoprecipitation applications (Kveiborg, M., et. al. (2005). Cancer Res. 65(11):4754-61).Inhibition Analysis: A representative inhibited ADAM 12 induced gelatin degradation. (Albrechtsen, R., et. al. (2013). J Cell Sci. 126(Pt 20):4707-20). Flow Cytometry Analysis: 1μg from a representative lot detected ADAM 12 in one million ADAM 12-DeltaCyt MCF-7 cells.Immunocytochemistry Analysis: A representative lot detected ADAM 12 in Immunocytochemistry applications (Sundberg, C., et. al. (2004). J Biol Chem. 279(49):51601-11).Western Blotting Analysis: A representative lot detected ADAM 12 in Western Blotting applications (Kveiborg, M., et. al. (2005). Cancer Res. 65(11):4754-61).
Gene Alias:
Disintegrin and metalloproteinase domain-containing protein 12;Meltrin-alpha
Host Species:
Mouse
Purification Method:
Protein G purified
Regulatory Status:
RUO
Primary or Secondary:
Primary
Test Specificity:
Clone 8F8 detects ADAM 12-DeltaCyt secreted form in human cells. It targets an epitope with in the prodomain region.
Content And Storage:
Stable for 1 year at 2-8°C from date of receipt.
Isotype:
IgG1 κ
Antigen: ADAM 12
Classification: Monoclonal
Conjugate: Unconjugated
Formulation: Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Gene Symbols: ADAM12;MLTN;UNQ346/PRO545
Immunogen: A full-length recombinant human ADAM12 secreted form expressed in HEK293 cells.
Quantity: 100 μg
Research Discipline: Apoptosis & Cancer
Gene ID (Entrez): NP_001275903
Target Species: Human
Form: Purified
Applications: Flow Cytometry, Immunocytochemistry, Immunoprecipitation, Western Blot
Clone: 6E6
Dilution: Flow Cytometry Analysis: 1μg from a representative lot detected ADAM 12 in one million ADAM 12-DeltaCyt MCF-7 cells.Western Blotting Analysis: A representative lot detected ADAM 12 in Western Blotting applications.Immunoprecipitation Analysis: A representative lot immunoprecipitated ADAM 12 in Immunoprecipitation applications.Immunocytochemistry Analysis: A representative lot detected ADAM 12 in Immunocytochemistry applications.Flow Cytometry Analysis: A representative lot detected ADAM 12 in Flow cytometry applications.
Gene Alias: Disintegrin and metalloproteinase domain-containing protein 12;Meltrin-alpha
Host Species: Mouse
Purification Method: Protein G purified
Regulatory Status: RUO
Primary or Secondary: Primary
Test Specificity: Clone 6E6 detects ADAM 12-DeltaCyt. It targets an epitope with in extracellular disintegrin-cysteine-rich domains.
Content And Storage: Stable for 1 year at 2-8°C from date of receipt.
Isotype: IgG1 κ
Antigen:
ADAM 12
Classification:
Monoclonal
Conjugate:
Unconjugated
Formulation:
Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Gene Symbols:
ADAM12;MLTN;UNQ346/PRO545
Immunogen:
A full-length recombinant human ADAM12 secreted form expressed in HEK293 cells.
Quantity:
100 μg
Research Discipline:
Apoptosis & Cancer
Gene ID (Entrez):
NP_001275903
Target Species:
Human
Form:
Purified
Applications:
Flow Cytometry, Immunocytochemistry, Immunoprecipitation, Western Blot
Clone:
6E6
Dilution:
Flow Cytometry Analysis: 1μg from a representative lot detected ADAM 12 in one million ADAM 12-DeltaCyt MCF-7 cells.Western Blotting Analysis: A representative lot detected ADAM 12 in Western Blotting applications.Immunoprecipitation Analysis: A representative lot immunoprecipitated ADAM 12 in Immunoprecipitation applications.Immunocytochemistry Analysis: A representative lot detected ADAM 12 in Immunocytochemistry applications.Flow Cytometry Analysis: A representative lot detected ADAM 12 in Flow cytometry applications.
Gene Alias:
Disintegrin and metalloproteinase domain-containing protein 12;Meltrin-alpha
Host Species:
Mouse
Purification Method:
Protein G purified
Regulatory Status:
RUO
Primary or Secondary:
Primary
Test Specificity:
Clone 6E6 detects ADAM 12-DeltaCyt. It targets an epitope with in extracellular disintegrin-cysteine-rich domains.
Content And Storage:
Stable for 1 year at 2-8°C from date of receipt.
Isotype:
IgG1 κ
Antigen: ADAM 12
Classification: Monoclonal
Conjugate: Unconjugated
Formulation: Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Gene Symbols: ADAM12;MLTN;UNQ346/PRO545
Immunogen: A full-length recombinant human ADAM12 secreted form expressed in HEK293 cells.
Quantity: 25 μg
Research Discipline: Apoptosis & Cancer
Gene ID (Entrez): NP_001275903
Target Species: Human
Form: Purified
Applications: Flow Cytometry, Immunocytochemistry, Immunoprecipitation, Western Blot
Clone: 6E6
Dilution: Flow Cytometry Analysis: 1μg from a representative lot detected ADAM 12 in one million ADAM 12-DeltaCyt MCF-7 cells.Western Blotting Analysis: A representative lot detected ADAM 12 in Western Blotting applications.Immunoprecipitation Analysis: A representative lot immunoprecipitated ADAM 12 in Immunoprecipitation applications.Immunocytochemistry Analysis: A representative lot detected ADAM 12 in Immunocytochemistry applications.Flow Cytometry Analysis: A representative lot detected ADAM 12 in Flow cytometry applications.
Gene Alias: Disintegrin and metalloproteinase domain-containing protein 12;Meltrin-alpha
Host Species: Mouse
Purification Method: Protein G purified
Regulatory Status: RUO
Primary or Secondary: Primary
Test Specificity: Clone 6E6 detects ADAM 12-DeltaCyt. It targets an epitope with in extracellular disintegrin-cysteine-rich domains.
Content And Storage: Stable for 1 year at 2-8°C from date of receipt.
Isotype: IgG1 κ
Antigen:
ADAM 12
Classification:
Monoclonal
Conjugate:
Unconjugated
Formulation:
Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Gene Symbols:
ADAM12;MLTN;UNQ346/PRO545
Immunogen:
A full-length recombinant human ADAM12 secreted form expressed in HEK293 cells.
Quantity:
25 μg
Research Discipline:
Apoptosis & Cancer
Gene ID (Entrez):
NP_001275903
Target Species:
Human
Form:
Purified
Applications:
Flow Cytometry, Immunocytochemistry, Immunoprecipitation, Western Blot
Clone:
6E6
Dilution:
Flow Cytometry Analysis: 1μg from a representative lot detected ADAM 12 in one million ADAM 12-DeltaCyt MCF-7 cells.Western Blotting Analysis: A representative lot detected ADAM 12 in Western Blotting applications.Immunoprecipitation Analysis: A representative lot immunoprecipitated ADAM 12 in Immunoprecipitation applications.Immunocytochemistry Analysis: A representative lot detected ADAM 12 in Immunocytochemistry applications.Flow Cytometry Analysis: A representative lot detected ADAM 12 in Flow cytometry applications.
Gene Alias:
Disintegrin and metalloproteinase domain-containing protein 12;Meltrin-alpha
Host Species:
Mouse
Purification Method:
Protein G purified
Regulatory Status:
RUO
Primary or Secondary:
Primary
Test Specificity:
Clone 6E6 detects ADAM 12-DeltaCyt. It targets an epitope with in extracellular disintegrin-cysteine-rich domains.
Content And Storage:
Stable for 1 year at 2-8°C from date of receipt.
Isotype:
IgG1 κ