MABT1331100
ADAM 12, Mouse anti-Human, Clone: 6E6, Millipore Sigma™
Manufacturer: Fischer Scientific
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | MABT1331100-Each-of-1 | In Stock | ₹ 43,067.48 |
MABT1331100 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 43,067.48
GST (18%): ₹ 7,752.146
Total Price: ₹ 50,819.626
Antigen
ADAM 12
Classification
Monoclonal
Conjugate
Unconjugated
Formulation
Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Gene Symbols
ADAM12;MLTN;UNQ346/PRO545
Immunogen
A full-length recombinant human ADAM12 secreted form expressed in HEK293 cells.
Quantity
100 μg
Research Discipline
Apoptosis & Cancer
Gene ID (Entrez)
NP_001275903
Target Species
Human
Form
Purified
Applications
Flow Cytometry, Immunocytochemistry, Immunoprecipitation, Western Blot
Clone
6E6
Dilution
Flow Cytometry Analysis: 1μg from a representative lot detected ADAM 12 in one million ADAM 12-DeltaCyt MCF-7 cells.Western Blotting Analysis: A representative lot detected ADAM 12 in Western Blotting applications.Immunoprecipitation Analysis: A representative lot immunoprecipitated ADAM 12 in Immunoprecipitation applications.Immunocytochemistry Analysis: A representative lot detected ADAM 12 in Immunocytochemistry applications.Flow Cytometry Analysis: A representative lot detected ADAM 12 in Flow cytometry applications.
Gene Alias
Disintegrin and metalloproteinase domain-containing protein 12;Meltrin-alpha
Host Species
Mouse
Purification Method
Protein G purified
Regulatory Status
RUO
Primary or Secondary
Primary
Test Specificity
Clone 6E6 detects ADAM 12-DeltaCyt. It targets an epitope with in extracellular disintegrin-cysteine-rich domains.
Content And Storage
Stable for 1 year at 2-8°C from date of receipt.
Isotype
IgG1 κ
Related Products
Description
- This mouse monoclonal Anti-ADAM 12, clone 6E6, Cat
- No
- MABT1331, is a mouse monoclonal antibody that detects Disintegrin and metalloproteinase domain-containing protein 12 and has been tested for use in Flow Cytometry, Immunocytochemistry, Immunoprecipitation, and Western Blotting
- Disintegrin and metalloproteinase domain-containing protein 12 (UniProt: O43184, also known as ADAM 12, Meltrin-alpha) is encoded by the ADAM12 (also known as MLTN, UNQ346/PRO545) gene (Gene ID: 8038) in human
- ADAM (A disintegrin and metalloprotease) family consists of multidomain cell-surface proteins that are synthesized in their proform
- Four isoforms of ADAM12 have been described that are produced by alternative splicing
- Isoform 1 is a single-pass transmembrane protein and isoforms 2,3, and 4 are secreted proteins
- ADM12 is synthesized with a signal peptide (aa 1-28) and a propeptide (aa 29-207), which are subsequently cleaved off in the mature form
- Isoform 1 shown to be expressed in placenta and in skeletal, cardiac, and smooth muscle
- Isoform 2 is reported to be expressed only in placenta or in embryo and fetus
- ADAM12 contains a cysteine rich domain (aa 514-649) that support cell adhesion through syndecans and triggers signaling events that lead to beta-1 integrin-dependent cell spreading
- However, in carcinoma cells the binding of this domain to syndecans does not allow the integrin-mediated cell spreading
- The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme
- Dissociation of the cysteine from the zinc ion activates the enzyme
- Upregulation of ADAM12 has been reported in malignant tumors
- In human breast cancer cells expressing ADAM12, MMP-14 is shown to be recruited to the cell surface which results in ADAM12 activation and accelerates tumor progression
- ADAM12 does not affect tumor cell proliferation, but it causes a diminution in tumor cell apoptosis and increases stromal cell apoptosis
- (Ref.: Sundberg, C., et al
- (2004)
- J
- Biol
- Chem
- 279(49); 51601 51611; Albrechtsen, R., et al
- (2013)
- J
- Cell Sci
- 126(20); 4707 4720; Kveiborg, M., et al
- (2005)
- Cancer Res
- 65(11); 4754-4761).
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Antigen: ADAM 12
Classification: Monoclonal
Conjugate: Unconjugated
Formulation: Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Gene Symbols: ADAM12;MLTN;UNQ346/PRO545
Immunogen: A full-length recombinant human ADAM12 secreted form expressed in HEK293 cells.
Quantity: 100 μg
Research Discipline: Apoptosis & Cancer
Gene ID (Entrez): NP_001275903
Target Species: Human
Form: Purified
Applications: Flow Cytometry, Immunocytochemistry, Immunoprecipitation, Western Blot
Clone: 6E6
Dilution: Flow Cytometry Analysis: 1μg from a representative lot detected ADAM 12 in one million ADAM 12-DeltaCyt MCF-7 cells.Western Blotting Analysis: A representative lot detected ADAM 12 in Western Blotting applications.Immunoprecipitation Analysis: A representative lot immunoprecipitated ADAM 12 in Immunoprecipitation applications.Immunocytochemistry Analysis: A representative lot detected ADAM 12 in Immunocytochemistry applications.Flow Cytometry Analysis: A representative lot detected ADAM 12 in Flow cytometry applications.
Gene Alias: Disintegrin and metalloproteinase domain-containing protein 12;Meltrin-alpha
Host Species: Mouse
Purification Method: Protein G purified
Regulatory Status: RUO
Primary or Secondary: Primary
Test Specificity: Clone 6E6 detects ADAM 12-DeltaCyt. It targets an epitope with in extracellular disintegrin-cysteine-rich domains.
Content And Storage: Stable for 1 year at 2-8°C from date of receipt.
Isotype: IgG1 κ
Antigen:
ADAM 12
Classification:
Monoclonal
Conjugate:
Unconjugated
Formulation:
Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Gene Symbols:
ADAM12;MLTN;UNQ346/PRO545
Immunogen:
A full-length recombinant human ADAM12 secreted form expressed in HEK293 cells.
Quantity:
100 μg
Research Discipline:
Apoptosis & Cancer
Gene ID (Entrez):
NP_001275903
Target Species:
Human
Form:
Purified
Applications:
Flow Cytometry, Immunocytochemistry, Immunoprecipitation, Western Blot
Clone:
6E6
Dilution:
Flow Cytometry Analysis: 1μg from a representative lot detected ADAM 12 in one million ADAM 12-DeltaCyt MCF-7 cells.Western Blotting Analysis: A representative lot detected ADAM 12 in Western Blotting applications.Immunoprecipitation Analysis: A representative lot immunoprecipitated ADAM 12 in Immunoprecipitation applications.Immunocytochemistry Analysis: A representative lot detected ADAM 12 in Immunocytochemistry applications.Flow Cytometry Analysis: A representative lot detected ADAM 12 in Flow cytometry applications.
Gene Alias:
Disintegrin and metalloproteinase domain-containing protein 12;Meltrin-alpha
Host Species:
Mouse
Purification Method:
Protein G purified
Regulatory Status:
RUO
Primary or Secondary:
Primary
Test Specificity:
Clone 6E6 detects ADAM 12-DeltaCyt. It targets an epitope with in extracellular disintegrin-cysteine-rich domains.
Content And Storage:
Stable for 1 year at 2-8°C from date of receipt.
Isotype:
IgG1 κ
Antigen: ADAM 12
Classification: Monoclonal
Conjugate: Unconjugated
Formulation: Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Gene Symbols: ADAM12;MLTN;UNQ346/PRO545
Immunogen: A full-length recombinant human ADAM12 secreted form expressed in HEK293 cells.
Quantity: 25 μg
Research Discipline: Apoptosis & Cancer
Gene ID (Entrez): NP_001275903
Target Species: Human
Form: Purified
Applications: Flow Cytometry, Immunocytochemistry, Immunoprecipitation, Western Blot
Clone: 6E6
Dilution: Flow Cytometry Analysis: 1μg from a representative lot detected ADAM 12 in one million ADAM 12-DeltaCyt MCF-7 cells.Western Blotting Analysis: A representative lot detected ADAM 12 in Western Blotting applications.Immunoprecipitation Analysis: A representative lot immunoprecipitated ADAM 12 in Immunoprecipitation applications.Immunocytochemistry Analysis: A representative lot detected ADAM 12 in Immunocytochemistry applications.Flow Cytometry Analysis: A representative lot detected ADAM 12 in Flow cytometry applications.
Gene Alias: Disintegrin and metalloproteinase domain-containing protein 12;Meltrin-alpha
Host Species: Mouse
Purification Method: Protein G purified
Regulatory Status: RUO
Primary or Secondary: Primary
Test Specificity: Clone 6E6 detects ADAM 12-DeltaCyt. It targets an epitope with in extracellular disintegrin-cysteine-rich domains.
Content And Storage: Stable for 1 year at 2-8°C from date of receipt.
Isotype: IgG1 κ
Antigen:
ADAM 12
Classification:
Monoclonal
Conjugate:
Unconjugated
Formulation:
Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Gene Symbols:
ADAM12;MLTN;UNQ346/PRO545
Immunogen:
A full-length recombinant human ADAM12 secreted form expressed in HEK293 cells.
Quantity:
25 μg
Research Discipline:
Apoptosis & Cancer
Gene ID (Entrez):
NP_001275903
Target Species:
Human
Form:
Purified
Applications:
Flow Cytometry, Immunocytochemistry, Immunoprecipitation, Western Blot
Clone:
6E6
Dilution:
Flow Cytometry Analysis: 1μg from a representative lot detected ADAM 12 in one million ADAM 12-DeltaCyt MCF-7 cells.Western Blotting Analysis: A representative lot detected ADAM 12 in Western Blotting applications.Immunoprecipitation Analysis: A representative lot immunoprecipitated ADAM 12 in Immunoprecipitation applications.Immunocytochemistry Analysis: A representative lot detected ADAM 12 in Immunocytochemistry applications.Flow Cytometry Analysis: A representative lot detected ADAM 12 in Flow cytometry applications.
Gene Alias:
Disintegrin and metalloproteinase domain-containing protein 12;Meltrin-alpha
Host Species:
Mouse
Purification Method:
Protein G purified
Regulatory Status:
RUO
Primary or Secondary:
Primary
Test Specificity:
Clone 6E6 detects ADAM 12-DeltaCyt. It targets an epitope with in extracellular disintegrin-cysteine-rich domains.
Content And Storage:
Stable for 1 year at 2-8°C from date of receipt.
Isotype:
IgG1 κ
Antigen: pan-Actin
Classification: Monoclonal
Conjugate: Unconjugated
Formulation: Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Gene Symbols: ACTA1;ACTA2;ACTB;ACTC1;ACTG1;ACTG2
Immunogen: KLH-conjugated linear peptide corresponding to a fragment from the C-terminal region of human Actin.
Quantity: 100 μL
Research Discipline: Cell Structure
Gene ID (Entrez): NP_001092
Target Species: Mouse, Rat, Drosophila, Hamster, Human, Monkey, Yeast
Form: Purified
Applications: Western Blot
Clone: 2A3
Dilution: Western Blotting Analysis: A 1:50 dilution from a representative lot detected Actins in various species samples (e.g. Human, rat, mouse, monkey, Hamster, Drosophila, Yeast) (Courtesy of Martina Mitterhuber, M.sc and Egon Ogris, M.D., Medical University of Vienna, Austria).
Gene Alias: Skeletal muscle alpha-actin;Smooth muscle alpha-actin;Cytoplasmic beta-actin;Cardiac muscle alpha-actin;Cytoplasmic gamma-actin;Smooth muscle gamma-actin
Host Species: Mouse
Purification Method: Protein G purified
Regulatory Status: RUO
Primary or Secondary: Primary
Test Specificity: Clone 2A3 detects all forms of actin in multiple species. It targets an epitope with in the C-terminal region.
Content And Storage: Stable for 1 year at 2-8°C from date of receipt.
Isotype: IgG1 κ
Antigen:
pan-Actin
Classification:
Monoclonal
Conjugate:
Unconjugated
Formulation:
Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Gene Symbols:
ACTA1;ACTA2;ACTB;ACTC1;ACTG1;ACTG2
Immunogen:
KLH-conjugated linear peptide corresponding to a fragment from the C-terminal region of human Actin.
Quantity:
100 μL
Research Discipline:
Cell Structure
Gene ID (Entrez):
NP_001092
Target Species:
Mouse, Rat, Drosophila, Hamster, Human, Monkey, Yeast
Form:
Purified
Applications:
Western Blot
Clone:
2A3
Dilution:
Western Blotting Analysis: A 1:50 dilution from a representative lot detected Actins in various species samples (e.g. Human, rat, mouse, monkey, Hamster, Drosophila, Yeast) (Courtesy of Martina Mitterhuber, M.sc and Egon Ogris, M.D., Medical University of Vienna, Austria).
Gene Alias:
Skeletal muscle alpha-actin;Smooth muscle alpha-actin;Cytoplasmic beta-actin;Cardiac muscle alpha-actin;Cytoplasmic gamma-actin;Smooth muscle gamma-actin
Host Species:
Mouse
Purification Method:
Protein G purified
Regulatory Status:
RUO
Primary or Secondary:
Primary
Test Specificity:
Clone 2A3 detects all forms of actin in multiple species. It targets an epitope with in the C-terminal region.
Content And Storage:
Stable for 1 year at 2-8°C from date of receipt.
Isotype:
IgG1 κ
Antigen: pan-Actin
Classification: Monoclonal
Conjugate: Unconjugated
Formulation: Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Gene Symbols: ACTA1;ACTA2;ACTB;ACTC1;ACTG1;ACTG2
Immunogen: KLH-conjugated linear peptide corresponding to a fragment from the C-terminal region of human Actin.
Quantity: 25 μL
Research Discipline: Cell Structure
Gene ID (Entrez): NP_001092
Target Species: Mouse, Rat, Drosophila, Hamster, Human, Monkey, Yeast
Form: Purified
Applications: Western Blot
Clone: 2A3
Dilution: Western Blotting Analysis: A 1:50 dilution from a representative lot detected Actins in various species samples (e.g. Human, rat, mouse, monkey, Hamster, Drosophila, Yeast) (Courtesy of Martina Mitterhuber, M.sc and Egon Ogris, M.D., Medical University of Vienna, Austria).
Gene Alias: Skeletal muscle alpha-actin;Smooth muscle alpha-actin;Cytoplasmic beta-actin;Cardiac muscle alpha-actin;Cytoplasmic gamma-actin;Smooth muscle gamma-actin
Host Species: Mouse
Purification Method: Protein G purified
Regulatory Status: RUO
Primary or Secondary: Primary
Test Specificity: Clone 2A3 detects all forms of actin in multiple species. It targets an epitope with in the C-terminal region.
Content And Storage: Stable for 1 year at 2-8°C from date of receipt.
Isotype: IgG1 κ
Antigen:
pan-Actin
Classification:
Monoclonal
Conjugate:
Unconjugated
Formulation:
Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Gene Symbols:
ACTA1;ACTA2;ACTB;ACTC1;ACTG1;ACTG2
Immunogen:
KLH-conjugated linear peptide corresponding to a fragment from the C-terminal region of human Actin.
Quantity:
25 μL
Research Discipline:
Cell Structure
Gene ID (Entrez):
NP_001092
Target Species:
Mouse, Rat, Drosophila, Hamster, Human, Monkey, Yeast
Form:
Purified
Applications:
Western Blot
Clone:
2A3
Dilution:
Western Blotting Analysis: A 1:50 dilution from a representative lot detected Actins in various species samples (e.g. Human, rat, mouse, monkey, Hamster, Drosophila, Yeast) (Courtesy of Martina Mitterhuber, M.sc and Egon Ogris, M.D., Medical University of Vienna, Austria).
Gene Alias:
Skeletal muscle alpha-actin;Smooth muscle alpha-actin;Cytoplasmic beta-actin;Cardiac muscle alpha-actin;Cytoplasmic gamma-actin;Smooth muscle gamma-actin
Host Species:
Mouse
Purification Method:
Protein G purified
Regulatory Status:
RUO
Primary or Secondary:
Primary
Test Specificity:
Clone 2A3 detects all forms of actin in multiple species. It targets an epitope with in the C-terminal region.
Content And Storage:
Stable for 1 year at 2-8°C from date of receipt.
Isotype:
IgG1 κ