MABT338100
phospho-cytokeratin-8 (K8) (Ser431), Mouse anti-Human, Mouse, Clone: 5B3, Millipore Sigma™
Manufacturer: Fischer Scientific
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| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | MABT338100-Each-of-1 | In Stock | ₹ 41,308.37 |
MABT338100 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 41,308.37
GST (18%): ₹ 7,435.507
Total Price: ₹ 48,743.877
Antigen
phospho-cytokeratin-8 (K8) (Ser431)
Classification
Monoclonal
Conjugate
Unconjugated
Formulation
Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Gene Symbols
KRT8;CYK8
Immunogen
Cytokeratin-8/18 purified from okadaic acid-treated HT29 cells.
Quantity
100 μg
Research Discipline
Cell Structure
Gene ID (Entrez)
NP_001243222
Target Species
Human, Mouse
Form
Purified
Applications
Immunofluorescence, Western Blot
Clone
5B3
Dilution
Immunofluorescence Analysis: A representative lot detected phospho-cytokeratin-8 (K8) (Ser431) in Immunofluorescence applications (Ku, N.O., et. al. (1997). J Biol Chem. 272(11):7556-64).Western Blotting Analysis: A representative lot detected phospho-cytokeratin-8 (K8) (Ser431) in Western Blotting applications (Yoon, K.H., et. al. (2001). J Cell Biol. 153(3):503-16; Ridge, K.M., et. al. (2005). J Biol Chem. 280(34):30400-5; Ku, N.O., et. al. (1997). J Biol Chem. 272(11):7556-64).
Gene Alias
Keratin;type II cytoskeletal 8;CK-8;Keratin-8;K8;Type-II keratin Kb80
Host Species
Mouse
Purification Method
Protein G purified
Regulatory Status
RUO
Primary or Secondary
Primary
Test Specificity
Clone 5B3 specifically detects cytokeratin-8 phosphorylated on serine 431.
Content And Storage
Stable for 1 year at 2-8°C from date of receipt.
Isotype
IgG1 κ
Related Products
Description
- Anti-phospho-cytokeratin-8 (K8) (Ser431), clone 5B3, Cat
- No
- MABT338, is a mouse monoclonal antibody that detects type II Cytokeratin-8 phopshorylated on serine 431 and has been tested for use in Immunofluorescence and Western Blotting
- Keratin, type II cytoskeletal 8 (UniProt: P05787; also known as Cytokeratin-8, CK-8 Keratin-8, K8, Type-II keratin Kb80) is encoded by the KRT8 (also known as CYK8) gene (Gene ID: 3856) in human
- Cytokeratin-8 is a type II, neutral to basic, protein of the intermediate filament family that together with Cytokeratin-19 (KRT19) helps to link the contractile apparatus to dystrophin at the costameres of striated muscle
- Cytokeratin-8 can undergo phosphorylation on three major serine residues: Serine 23, 431, and 73
- Serine 23 is shown to be highly conserved in all type II keratins
- Phosphorylation at Serine 431 is reported to increase during mitosis and upon treatment with epidermal growth factor
- Cytokeratin-8 can also undergo O-glycosylation in a cell cycle-dependent manner and glycosylation increases its solubility and reduces stability by inducing proteasomal degradation
- Mutations in KRT8 gene have been linked to liver cirrhosis that is characterized by severe panlobular liver-cell swelling with Mallory body formation, prominent pericellular fibrosis, and marked deposits of copper
- Two isoforms of Cytokeratin-8 have been described that are produced by alternative splicing.
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Antigen: phospho-cytokeratin-8 (K8) (Ser431)
Classification: Monoclonal
Conjugate: Unconjugated
Formulation: Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Gene Symbols: KRT8;CYK8
Immunogen: Cytokeratin-8/18 purified from okadaic acid-treated HT29 cells.
Quantity: 100 μg
Research Discipline: Cell Structure
Gene ID (Entrez): NP_001243222
Target Species: Human, Mouse
Form: Purified
Applications: Immunofluorescence, Western Blot
Clone: 5B3
Dilution: Immunofluorescence Analysis: A representative lot detected phospho-cytokeratin-8 (K8) (Ser431) in Immunofluorescence applications (Ku, N.O., et. al. (1997). J Biol Chem. 272(11):7556-64).Western Blotting Analysis: A representative lot detected phospho-cytokeratin-8 (K8) (Ser431) in Western Blotting applications (Yoon, K.H., et. al. (2001). J Cell Biol. 153(3):503-16; Ridge, K.M., et. al. (2005). J Biol Chem. 280(34):30400-5; Ku, N.O., et. al. (1997). J Biol Chem. 272(11):7556-64).
Gene Alias: Keratin;type II cytoskeletal 8;CK-8;Keratin-8;K8;Type-II keratin Kb80
Host Species: Mouse
Purification Method: Protein G purified
Regulatory Status: RUO
Primary or Secondary: Primary
Test Specificity: Clone 5B3 specifically detects cytokeratin-8 phosphorylated on serine 431.
Content And Storage: Stable for 1 year at 2-8°C from date of receipt.
Isotype: IgG1 κ
Antigen:
phospho-cytokeratin-8 (K8) (Ser431)
Classification:
Monoclonal
Conjugate:
Unconjugated
Formulation:
Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Gene Symbols:
KRT8;CYK8
Immunogen:
Cytokeratin-8/18 purified from okadaic acid-treated HT29 cells.
Quantity:
100 μg
Research Discipline:
Cell Structure
Gene ID (Entrez):
NP_001243222
Target Species:
Human, Mouse
Form:
Purified
Applications:
Immunofluorescence, Western Blot
Clone:
5B3
Dilution:
Immunofluorescence Analysis: A representative lot detected phospho-cytokeratin-8 (K8) (Ser431) in Immunofluorescence applications (Ku, N.O., et. al. (1997). J Biol Chem. 272(11):7556-64).Western Blotting Analysis: A representative lot detected phospho-cytokeratin-8 (K8) (Ser431) in Western Blotting applications (Yoon, K.H., et. al. (2001). J Cell Biol. 153(3):503-16; Ridge, K.M., et. al. (2005). J Biol Chem. 280(34):30400-5; Ku, N.O., et. al. (1997). J Biol Chem. 272(11):7556-64).
Gene Alias:
Keratin;type II cytoskeletal 8;CK-8;Keratin-8;K8;Type-II keratin Kb80
Host Species:
Mouse
Purification Method:
Protein G purified
Regulatory Status:
RUO
Primary or Secondary:
Primary
Test Specificity:
Clone 5B3 specifically detects cytokeratin-8 phosphorylated on serine 431.
Content And Storage:
Stable for 1 year at 2-8°C from date of receipt.
Isotype:
IgG1 κ
Antigen: phospho-cytokeratin-8 (K8) (Ser431)
Classification: Monoclonal
Conjugate: Unconjugated
Formulation: Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Gene Symbols: KRT8;CYK8
Immunogen: Cytokeratin-8/18 purified from okadaic acid-treated HT29 cells.
Quantity: 25 μg
Research Discipline: Cell Structure
Gene ID (Entrez): NP_001243222
Target Species: Human, Mouse
Form: Purified
Applications: Immunofluorescence, Western Blot
Clone: 5B3
Dilution: Immunofluorescence Analysis: A representative lot detected phospho-cytokeratin-8 (K8) (Ser431) in Immunofluorescence applications (Ku, N.O., et. al. (1997). J Biol Chem. 272(11):7556-64).Western Blotting Analysis: A representative lot detected phospho-cytokeratin-8 (K8) (Ser431) in Western Blotting applications (Yoon, K.H., et. al. (2001). J Cell Biol. 153(3):503-16; Ridge, K.M., et. al. (2005). J Biol Chem. 280(34):30400-5; Ku, N.O., et. al. (1997). J Biol Chem. 272(11):7556-64).
Gene Alias: Keratin;type II cytoskeletal 8;CK-8;Keratin-8;K8;Type-II keratin Kb80
Host Species: Mouse
Purification Method: Protein G purified
Regulatory Status: RUO
Primary or Secondary: Primary
Test Specificity: Clone 5B3 specifically detects cytokeratin-8 phosphorylated on serine 431.
Content And Storage: Stable for 1 year at 2-8°C from date of receipt.
Isotype: IgG1 κ
Antigen:
phospho-cytokeratin-8 (K8) (Ser431)
Classification:
Monoclonal
Conjugate:
Unconjugated
Formulation:
Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Gene Symbols:
KRT8;CYK8
Immunogen:
Cytokeratin-8/18 purified from okadaic acid-treated HT29 cells.
Quantity:
25 μg
Research Discipline:
Cell Structure
Gene ID (Entrez):
NP_001243222
Target Species:
Human, Mouse
Form:
Purified
Applications:
Immunofluorescence, Western Blot
Clone:
5B3
Dilution:
Immunofluorescence Analysis: A representative lot detected phospho-cytokeratin-8 (K8) (Ser431) in Immunofluorescence applications (Ku, N.O., et. al. (1997). J Biol Chem. 272(11):7556-64).Western Blotting Analysis: A representative lot detected phospho-cytokeratin-8 (K8) (Ser431) in Western Blotting applications (Yoon, K.H., et. al. (2001). J Cell Biol. 153(3):503-16; Ridge, K.M., et. al. (2005). J Biol Chem. 280(34):30400-5; Ku, N.O., et. al. (1997). J Biol Chem. 272(11):7556-64).
Gene Alias:
Keratin;type II cytoskeletal 8;CK-8;Keratin-8;K8;Type-II keratin Kb80
Host Species:
Mouse
Purification Method:
Protein G purified
Regulatory Status:
RUO
Primary or Secondary:
Primary
Test Specificity:
Clone 5B3 specifically detects cytokeratin-8 phosphorylated on serine 431.
Content And Storage:
Stable for 1 year at 2-8°C from date of receipt.
Isotype:
IgG1 κ
Antigen: Proteinase 3/PR3
Classification: Monoclonal
Conjugate: Unconjugated
Formulation: Purified mouse monoclonal IgG1κ antibody in buffer containing 0.1M Tris-Glycine (pH 7.4), 150mM NaCl with 0.05% Sodium Azide.
Gene Symbols: __
Immunogen: Granule extract from HMC-1/PR3 mast cells expressing transfected human neutrophil PR3 (Sun, J., et al. (1998). J. Immunol. Methods. 211(1-2):111-123).
Quantity: 100 μg
Research Discipline: Cell Structure
Gene ID (Entrez): NP_002768
Target Species: Human
Form: Purified
Applications: ELISA, Flow Cytometry, Western Blot
Clone: MCPR3-2
Dilution: __
Gene Alias: __
Host Species: Mouse
Purification Method: Protein G Purified
Regulatory Status: RUO
Primary or Secondary: Primary
Test Specificity: Clone MCPR3-2 detected wild-type PR3 and the catalytically inactive S176A PR3 mutant, but not neutrophil elastase or cathepsin G. Clone MCPR3-2 did not interfere with PR3 enzymatic activity or its interaction with α1-protease inhibitor/α1-PI (Sun, J., et al. (1998). J. Immunol. Methods. 211(1-2):111-123). The ratios of OD readings obtained with PR3-complexed clone MCPR3-2 to the corresponding ODs obtained with PR3-complexed clone MCPR3-3 (Mfr. No. MABF973) in ELISA-based serum PR3 autoantibodies (ANCAs) measurements correlate well with the neutralizing activity of ANCAs in patients serum samples (Silva, F., et al. (2010). J. Autoimmun. 35(4) 299-308).
Content And Storage: Stable for 1 year at 2°-8°C from date of receipt.
Isotype: IgG1 κ
Antigen:
Proteinase 3/PR3
Classification:
Monoclonal
Conjugate:
Unconjugated
Formulation:
Purified mouse monoclonal IgG1κ antibody in buffer containing 0.1M Tris-Glycine (pH 7.4), 150mM NaCl with 0.05% Sodium Azide.
Gene Symbols:
__
Immunogen:
Granule extract from HMC-1/PR3 mast cells expressing transfected human neutrophil PR3 (Sun, J., et al. (1998). J. Immunol. Methods. 211(1-2):111-123).
Quantity:
100 μg
Research Discipline:
Cell Structure
Gene ID (Entrez):
NP_002768
Target Species:
Human
Form:
Purified
Applications:
ELISA, Flow Cytometry, Western Blot
Clone:
MCPR3-2
Dilution:
__
Gene Alias:
__
Host Species:
Mouse
Purification Method:
Protein G Purified
Regulatory Status:
RUO
Primary or Secondary:
Primary
Test Specificity:
Clone MCPR3-2 detected wild-type PR3 and the catalytically inactive S176A PR3 mutant, but not neutrophil elastase or cathepsin G. Clone MCPR3-2 did not interfere with PR3 enzymatic activity or its interaction with α1-protease inhibitor/α1-PI (Sun, J., et al. (1998). J. Immunol. Methods. 211(1-2):111-123). The ratios of OD readings obtained with PR3-complexed clone MCPR3-2 to the corresponding ODs obtained with PR3-complexed clone MCPR3-3 (Mfr. No. MABF973) in ELISA-based serum PR3 autoantibodies (ANCAs) measurements correlate well with the neutralizing activity of ANCAs in patients serum samples (Silva, F., et al. (2010). J. Autoimmun. 35(4) 299-308).
Content And Storage:
Stable for 1 year at 2°-8°C from date of receipt.
Isotype:
IgG1 κ
Antigen: phospho-cytokeratin-8 (K8) (Ser73)
Classification: Monoclonal
Conjugate: Unconjugated
Formulation: Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Gene Symbols: KRT8;CYK8
Immunogen: Cytokeratin-8 purified from HT29 cells treated with okadaic acid (1 mg/ml, 2h).
Quantity: 100 μg
Research Discipline: Cell Structure
Gene ID (Entrez): NP_001243222
Target Species: Mouse, Human
Form: Purified
Applications: ELISA, Immunocytochemistry, Immunofluorescence
Clone: LJ4
Dilution: Immunofluorescence Analysis: A representative lot detected phospho-cytokeratin-8 (K8) (Ser73) in Immunofluorescence applications (McIntosh, P.B., et. al. (2010). J Cell Sci. 123(Pt 16):2810-22; Liao, J., et. al. (1997). J Biol Chem. 272(28):17565-73; Ku, N.O., et. al. (2003). Hepatology. 37(5):1006-14; Toivola, D.M., et. al. (2002). Mol Biol Cell. 13(6):1857-70).Immunoprecipitation Analysis: A representative lot immunoprecipitaated phospho-cytokeratin-8 (K8) (Ser73) in Immunoprecipitation applications (Ku, N.O., et. al. (2003). Hepatology. 37(5):1006-14; Liao, J., et. al. (1997). J Biol Chem. 272(28):17565-73).Immunocytochemistry Analysis: A representative lot detected phospho-cytokeratin-8 (K8) (Ser73) in Immunocytochemistry applications (Toivola, D.M., et. al. (2002). Mol Biol Cell. 13(6):1857-70).Western Blotting Analysis: A representative lot detected phospho-cytokeratin-8 (K8) (Ser73) in Western Blotting applications (Toivola, D.M., et. al. (2002). Mol Biol Cell. 13(6):1857-70; Ku
Gene Alias: Keratin;type II cytoskeletal 8;CK-8;Keratin-8;K8;Type-II keratin Kb80
Host Species: Mouse
Purification Method: Protein G purified
Regulatory Status: RUO
Primary or Secondary: Primary
Test Specificity: Clone LJ4 detects human cytokeratin-8 phosphorylated on serine 73 and murine cytokeratin-8 phosphorylated on serine 79.
Content And Storage: Stable for 1 year at 2-8°C from date of receipt.
Isotype: IgG1 κ
Antigen:
phospho-cytokeratin-8 (K8) (Ser73)
Classification:
Monoclonal
Conjugate:
Unconjugated
Formulation:
Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Gene Symbols:
KRT8;CYK8
Immunogen:
Cytokeratin-8 purified from HT29 cells treated with okadaic acid (1 mg/ml, 2h).
Quantity:
100 μg
Research Discipline:
Cell Structure
Gene ID (Entrez):
NP_001243222
Target Species:
Mouse, Human
Form:
Purified
Applications:
ELISA, Immunocytochemistry, Immunofluorescence
Clone:
LJ4
Dilution:
Immunofluorescence Analysis: A representative lot detected phospho-cytokeratin-8 (K8) (Ser73) in Immunofluorescence applications (McIntosh, P.B., et. al. (2010). J Cell Sci. 123(Pt 16):2810-22; Liao, J., et. al. (1997). J Biol Chem. 272(28):17565-73; Ku, N.O., et. al. (2003). Hepatology. 37(5):1006-14; Toivola, D.M., et. al. (2002). Mol Biol Cell. 13(6):1857-70).Immunoprecipitation Analysis: A representative lot immunoprecipitaated phospho-cytokeratin-8 (K8) (Ser73) in Immunoprecipitation applications (Ku, N.O., et. al. (2003). Hepatology. 37(5):1006-14; Liao, J., et. al. (1997). J Biol Chem. 272(28):17565-73).Immunocytochemistry Analysis: A representative lot detected phospho-cytokeratin-8 (K8) (Ser73) in Immunocytochemistry applications (Toivola, D.M., et. al. (2002). Mol Biol Cell. 13(6):1857-70).Western Blotting Analysis: A representative lot detected phospho-cytokeratin-8 (K8) (Ser73) in Western Blotting applications (Toivola, D.M., et. al. (2002). Mol Biol Cell. 13(6):1857-70; Ku
Gene Alias:
Keratin;type II cytoskeletal 8;CK-8;Keratin-8;K8;Type-II keratin Kb80
Host Species:
Mouse
Purification Method:
Protein G purified
Regulatory Status:
RUO
Primary or Secondary:
Primary
Test Specificity:
Clone LJ4 detects human cytokeratin-8 phosphorylated on serine 73 and murine cytokeratin-8 phosphorylated on serine 79.
Content And Storage:
Stable for 1 year at 2-8°C from date of receipt.
Isotype:
IgG1 κ