MABT340MI
MilliporeSigma™ Proteinase 3/PR3, Mouse, Unlabeled, Clone: MCPR3-2,
Manufacturer: MilliporeSigma™
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | MABT340MI-Each-of-1 | In Stock | ₹ 38,734.72 |
MABT340MI - Each of 1
In Stock
Quantity
1
Base Price: ₹ 38,734.72
GST (18%): ₹ 6,972.25
Total Price: ₹ 45,706.97
Antigen
Proteinase 3/PR3
Classification
Monoclonal
Concentration
Please refer to lot specific datasheet.
Formulation
Purified mouse monoclonal IgG1κ antibody in buffer containing 0.1M Tris-Glycine (pH 7.4), 150mM NaCl with 0.05% Sodium Azide.
Gene Accession No.
P24158
Immunogen
Granule extract from HMC-1/PR3 mast cells expressing transfected human neutrophil PR3 (Sun, J., et al. (1998). J. Immunol. Methods. 211(1-2):111-123).
Quantity
100 μg
Research Discipline
Cell Structure
Gene ID (Entrez)
NP_002768
Target Species
Human
Form
Purified
Applications
ELISA, Flow Cytometry, Western Blot
Clone
MCPR3-2
Conjugate
Unconjugated
Gene
PRTN3, CANCA, MBN, NP4
Host Species
Mouse
Purification Method
Protein G Purified
Regulatory Status
RUO
Primary or Secondary
Primary
Test Specificity
Clone MCPR3-2 detected wild-type PR3 and the catalytically inactive S176A PR3 mutant, but not neutrophil elastase or cathepsin G. Clone MCPR3-2 did not interfere with PR3 enzymatic activity or its interaction with α1-protease inhibitor/α1-PI (Sun, J., et al. (1998). J. Immunol. Methods. 211(1-2):111-123). The ratios of OD readings obtained with PR3-complexed clone MCPR3-2 to the corresponding ODs obtained with PR3-complexed clone MCPR3-3 (Mfr. No. MABF973) in ELISA-based serum PR3 autoantibodies (ANCAs) measurements correlate well with the neutralizing activity of ANCAs in patients serum samples (Silva, F., et al. (2010). J. Autoimmun. 35(4) 299-308).
Content And Storage
Stable for 1 year at 2°-8°C from date of receipt.
Isotype
IgG1 κ
Related Products
Description
- Myeloblastin (EC 3.4.21.76; UniProt P24158; also known as AGP7, C-ANCA antigen, Leukocyte proteinase 3, Neutrophil proteinase 4, NP-4, P29, PR-3, PR3, Wegener autoantigen) is encoded by the PRTN3 (also known as CANCA, MBN, NP4) gene (Gene ID 5657) in human
- Proteinase 3 (PR3) is one of four neutral serine proteases (elastase, cathepsin G, PR3, and neutrophil serine protease 4) stored as fully processed mature enzymes in azurophil granules of human neutrophils
- Instead of being targeted to granules after their synthesis, some PR3 molecules end up on the surface of neutrophil plasma membrane in either pro- or mature form
- The degree of such surface expression is genetically determined, but the surface exposure and pericellular activity of PR3 around neutrophils can be further upregulated upon neutrophil priming and activation
- PR3 autoantibody (anti-neutrophil cytoplasmic antibodies or ANCA) is the main pathogenic feature in patients suffering from granulomatosis with polyangiitis (GPA; formerly called Wegener granulomatosis)
- ANCAs are shown to activate cytokine-primed neutrophils in vitro by cross-linking surface-exposed PR3 and Fcγ receptors
- PR3 activity and/or its inactivation by alpha 1-antitrypsin/alpha-1 proteinase inhibitor (α1PI) varies in the human population and contributes to the risk for GPA manifestations either at onset, during relapses, or during systemic progression
- PR3 is initially produced with a signal peptide sequence (a.a
- 1-25), an N-terminal and a C-terminal propeptide sequence (a.a
- 26-27 and 249-256, respectively), the removal of which yields the mature enzyme (a.a
- 28-248).
Compare Similar Items
Show Difference
Antigen: Proteinase 3/PR3
Classification: Monoclonal
Concentration: Please refer to lot specific datasheet.
Formulation: Purified mouse monoclonal IgG1κ antibody in buffer containing 0.1M Tris-Glycine (pH 7.4), 150mM NaCl with 0.05% Sodium Azide.
Gene Accession No.: P24158
Immunogen: Granule extract from HMC-1/PR3 mast cells expressing transfected human neutrophil PR3 (Sun, J., et al. (1998). J. Immunol. Methods. 211(1-2):111-123).
Quantity: 100 μg
Research Discipline: Cell Structure
Gene ID (Entrez): NP_002768
Target Species: Human
Form: Purified
Applications: ELISA, Flow Cytometry, Western Blot
Clone: MCPR3-2
Conjugate: Unconjugated
Gene: PRTN3, CANCA, MBN, NP4
Host Species: Mouse
Purification Method: Protein G Purified
Regulatory Status: RUO
Primary or Secondary: Primary
Test Specificity: Clone MCPR3-2 detected wild-type PR3 and the catalytically inactive S176A PR3 mutant, but not neutrophil elastase or cathepsin G. Clone MCPR3-2 did not interfere with PR3 enzymatic activity or its interaction with α1-protease inhibitor/α1-PI (Sun, J., et al. (1998). J. Immunol. Methods. 211(1-2):111-123). The ratios of OD readings obtained with PR3-complexed clone MCPR3-2 to the corresponding ODs obtained with PR3-complexed clone MCPR3-3 (Mfr. No. MABF973) in ELISA-based serum PR3 autoantibodies (ANCAs) measurements correlate well with the neutralizing activity of ANCAs in patients serum samples (Silva, F., et al. (2010). J. Autoimmun. 35(4) 299-308).
Content And Storage: Stable for 1 year at 2°-8°C from date of receipt.
Isotype: IgG1 κ
Antigen:
Proteinase 3/PR3
Classification:
Monoclonal
Concentration:
Please refer to lot specific datasheet.
Formulation:
Purified mouse monoclonal IgG1κ antibody in buffer containing 0.1M Tris-Glycine (pH 7.4), 150mM NaCl with 0.05% Sodium Azide.
Gene Accession No.:
P24158
Immunogen:
Granule extract from HMC-1/PR3 mast cells expressing transfected human neutrophil PR3 (Sun, J., et al. (1998). J. Immunol. Methods. 211(1-2):111-123).
Quantity:
100 μg
Research Discipline:
Cell Structure
Gene ID (Entrez):
NP_002768
Target Species:
Human
Form:
Purified
Applications:
ELISA, Flow Cytometry, Western Blot
Clone:
MCPR3-2
Conjugate:
Unconjugated
Gene:
PRTN3, CANCA, MBN, NP4
Host Species:
Mouse
Purification Method:
Protein G Purified
Regulatory Status:
RUO
Primary or Secondary:
Primary
Test Specificity:
Clone MCPR3-2 detected wild-type PR3 and the catalytically inactive S176A PR3 mutant, but not neutrophil elastase or cathepsin G. Clone MCPR3-2 did not interfere with PR3 enzymatic activity or its interaction with α1-protease inhibitor/α1-PI (Sun, J., et al. (1998). J. Immunol. Methods. 211(1-2):111-123). The ratios of OD readings obtained with PR3-complexed clone MCPR3-2 to the corresponding ODs obtained with PR3-complexed clone MCPR3-3 (Mfr. No. MABF973) in ELISA-based serum PR3 autoantibodies (ANCAs) measurements correlate well with the neutralizing activity of ANCAs in patients serum samples (Silva, F., et al. (2010). J. Autoimmun. 35(4) 299-308).
Content And Storage:
Stable for 1 year at 2°-8°C from date of receipt.
Isotype:
IgG1 κ
Antigen: phospho-cytokeratin-8 (K8) (Ser73)
Classification: Monoclonal
Concentration: __
Formulation: Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Gene Accession No.: __
Immunogen: Cytokeratin-8 purified from HT29 cells treated with okadaic acid (1 mg/ml, 2h).
Quantity: 100 μg
Research Discipline: Cell Structure
Gene ID (Entrez): NP_001243222
Target Species: Mouse, Human
Form: Purified
Applications: ELISA, Immunocytochemistry, Immunofluorescence
Clone: LJ4
Conjugate: Unconjugated
Gene: __
Host Species: Mouse
Purification Method: Protein G purified
Regulatory Status: RUO
Primary or Secondary: Primary
Test Specificity: Clone LJ4 detects human cytokeratin-8 phosphorylated on serine 73 and murine cytokeratin-8 phosphorylated on serine 79.
Content And Storage: Stable for 1 year at 2-8°C from date of receipt.
Isotype: IgG1 κ
Antigen:
phospho-cytokeratin-8 (K8) (Ser73)
Classification:
Monoclonal
Concentration:
__
Formulation:
Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Gene Accession No.:
__
Immunogen:
Cytokeratin-8 purified from HT29 cells treated with okadaic acid (1 mg/ml, 2h).
Quantity:
100 μg
Research Discipline:
Cell Structure
Gene ID (Entrez):
NP_001243222
Target Species:
Mouse, Human
Form:
Purified
Applications:
ELISA, Immunocytochemistry, Immunofluorescence
Clone:
LJ4
Conjugate:
Unconjugated
Gene:
__
Host Species:
Mouse
Purification Method:
Protein G purified
Regulatory Status:
RUO
Primary or Secondary:
Primary
Test Specificity:
Clone LJ4 detects human cytokeratin-8 phosphorylated on serine 73 and murine cytokeratin-8 phosphorylated on serine 79.
Content And Storage:
Stable for 1 year at 2-8°C from date of receipt.
Isotype:
IgG1 κ
Antigen: phospho-cytokeratin-8 (K8) (Ser73)
Classification: Monoclonal
Concentration: __
Formulation: Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Gene Accession No.: __
Immunogen: Cytokeratin-8 purified from HT29 cells treated with okadaic acid (1 mg/ml, 2h).
Quantity: 25 μg
Research Discipline: Cell Structure
Gene ID (Entrez): NP_001243222
Target Species: Mouse, Human
Form: Purified
Applications: ELISA, Immunocytochemistry, Immunofluorescence
Clone: LJ4
Conjugate: Unconjugated
Gene: __
Host Species: Mouse
Purification Method: Protein G purified
Regulatory Status: RUO
Primary or Secondary: Primary
Test Specificity: Clone LJ4 detects human cytokeratin-8 phosphorylated on serine 73 and murine cytokeratin-8 phosphorylated on serine 79.
Content And Storage: Stable for 1 year at 2-8°C from date of receipt.
Isotype: IgG1 κ
Antigen:
phospho-cytokeratin-8 (K8) (Ser73)
Classification:
Monoclonal
Concentration:
__
Formulation:
Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Gene Accession No.:
__
Immunogen:
Cytokeratin-8 purified from HT29 cells treated with okadaic acid (1 mg/ml, 2h).
Quantity:
25 μg
Research Discipline:
Cell Structure
Gene ID (Entrez):
NP_001243222
Target Species:
Mouse, Human
Form:
Purified
Applications:
ELISA, Immunocytochemistry, Immunofluorescence
Clone:
LJ4
Conjugate:
Unconjugated
Gene:
__
Host Species:
Mouse
Purification Method:
Protein G purified
Regulatory Status:
RUO
Primary or Secondary:
Primary
Test Specificity:
Clone LJ4 detects human cytokeratin-8 phosphorylated on serine 73 and murine cytokeratin-8 phosphorylated on serine 79.
Content And Storage:
Stable for 1 year at 2-8°C from date of receipt.
Isotype:
IgG1 κ
Antigen: Prelamin-A
Classification: Monoclonal
Concentration: __
Formulation: Purified rat monoclonal IgG2aκ antibody in buffer containing 0.1M Tris-Glycine (pH 7.4), 150mM NaCl with 0.05% sodium azide.
Gene Accession No.: P48678
Immunogen: Linear peptide corresponding to the C-terminal propeptide sequence of mouse Prelamin-A.
Quantity: 100 μg
Research Discipline: Cell Structure
Gene ID (Entrez): NP_001002011
Target Species: Mouse
Form: Purified
Applications: Immunofluorescence, Western Blot
Clone: 7G11
Conjugate: Unconjugated
Gene: __
Host Species: Rat
Purification Method: Protein G purified
Regulatory Status: RUO
Primary or Secondary: Primary
Test Specificity: Clone 7G11 detects prelamin-A regardless of its farnesylation state, but not mature Lamin-A (a.a. 1-647) lacking the C-terminal propeptide sequence. Clone 7G11 does not detect the pre- or mature forms of the spliced isoforms C1 & C2 (Lamin-C; P48678-2 and P48678-3).
Content And Storage: 2°C to 8°C for one year from date of shipment
Isotype: IgG2a κ
Antigen:
Prelamin-A
Classification:
Monoclonal
Concentration:
__
Formulation:
Purified rat monoclonal IgG2aκ antibody in buffer containing 0.1M Tris-Glycine (pH 7.4), 150mM NaCl with 0.05% sodium azide.
Gene Accession No.:
P48678
Immunogen:
Linear peptide corresponding to the C-terminal propeptide sequence of mouse Prelamin-A.
Quantity:
100 μg
Research Discipline:
Cell Structure
Gene ID (Entrez):
NP_001002011
Target Species:
Mouse
Form:
Purified
Applications:
Immunofluorescence, Western Blot
Clone:
7G11
Conjugate:
Unconjugated
Gene:
__
Host Species:
Rat
Purification Method:
Protein G purified
Regulatory Status:
RUO
Primary or Secondary:
Primary
Test Specificity:
Clone 7G11 detects prelamin-A regardless of its farnesylation state, but not mature Lamin-A (a.a. 1-647) lacking the C-terminal propeptide sequence. Clone 7G11 does not detect the pre- or mature forms of the spliced isoforms C1 & C2 (Lamin-C; P48678-2 and P48678-3).
Content And Storage:
2°C to 8°C for one year from date of shipment
Isotype:
IgG2a κ