MABE1112MI

MilliporeSigma™ PRMT9, Mouse, Unlabeled, Clone: 128-29-1,

Manufacturer: MilliporeSigma™

CAS Number: 128-29-1

Select a Size

Pack Size SKU Availability Price
Each of 1 MABE1112MI-Each-of-1 In Stock ₹ 38,734.72

MABE1112MI - Each of 1

₹ 38,734.72

In Stock

Quantity

1

Base Price: ₹ 38,734.72

GST (18%): ₹ 6,972.25

Total Price: ₹ 45,706.97

Antigen

PRMT9

Classification

Monoclonal

Concentration

Please refer to lot specific datasheet.

Formulation

Purified mouse monoclonal IgG1κ antibody in buffer containing 0.1M Tris-Glycine (pH 7.4), 150mM NaCl with 0.05% Sodium Azide.

Gene Accession No.

Q6P2P2

Immunogen

GST-tagged recombinant protein corresponding to the N-terminal half of human PRMT9.

Quantity

100 μg

Research Discipline

Epigenetics & Nuclear Function

Gene ID (Entrez)

NP_612373

Target Species

Human

Form

Purified

Applications

Immunohistochemistry, Immunoprecipitation, Western Blot

Clone

128-29-1

Conjugate

Unconjugated

Gene

PRMT9, PRMT10

Host Species

Mouse

Purification Method

Protein G Purified

Regulatory Status

RUO

Primary or Secondary

Primary

Test Specificity

Clone detected GST fusion containing recombinant human PRMT9 fragment a.a. 401-550, as well as endogenous human, but not mouse, PRMT9 in cell lysates (Yang, Y., et al. (2015). Nat. Commun. 6:6428). Epitope region is present in both spliced isoforms of human PRMT9 reported by UniProt (Q6P2P2).

Content And Storage

Stable for 1 year at 2°-8°C from date of receipt.

Isotype

IgG1 κ

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Description

  • Putative protein arginine N-methyltransferase 9 (UniProt Q6P2P2; also known as Putative protein arginine N-methyltransferase 10) is encoded by the PRMT9 (also known as PRMT10) gene (Gene ID 90826) in human
  • Mammalian protein arginine methyltransferases (PRMTs) catalyze the methylation of arginine residues on histone and non-histone proteins
  • PRMTs are divided into three types based on their mode of activity
  • Type I PRMTs (PRMT1–4, PRMT6, and PRMT8) transfer up to two methyl groups on the same terminal guanidino nitrogen of arginine to form ω-NG-monomethylarginine (MMA) and ω-NG,NG-asymmetric dimethylarginine (ADMA) residues
  • Type II PRMTs (PRMT5 and PRMT9) transfer methyl groups on different terminal guanidino nitrogens to form μμα and ω-NG,N'G-symmetric dimethylarginine (SDMA) residues, while type III PRMT7 transfers only one single methyl group to form MMA residues
  • PRMT9 is involved in alternative splicing regulation by modulating spliceosomal small nuclear ribonucleoprotein (snRNP) maturation in the cytoplasm
  • PRMT9 complexes with the splicing factors SF3B2 (SAP145) and SF3B4 (SAP49) and mediates SF3B2 Arg508 modification via MMA and SDMA
  • Similar to PRMT7, but unlike other PRMTs, PRMT9 contains a duplicated methyltransferase domain (a.a
  • 137-446 and 530-845), with the N-terminal MTase acting as a funtional AdoMet-binding domain and the C-terminal MTase domain serving a pseudodimer function, mimicking the homodimer formation of other PRMTs needed for subtrate processing
  • In addition, PRMT9 contains three tetratricopeptide repeats (TPR1-3; a.a
  • 25-58, 67-100, 101-134) known to mediate protein-protein interactions.

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MABE1112MI

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Antigen:
PRMT9

Classification:
Monoclonal

Concentration:
Please refer to lot specific datasheet.

Formulation:
Purified mouse monoclonal IgG1κ antibody in buffer containing 0.1M Tris-Glycine (pH 7.4), 150mM NaCl with 0.05% Sodium Azide.

Gene Accession No.:
Q6P2P2

Immunogen:
GST-tagged recombinant protein corresponding to the N-terminal half of human PRMT9.

Quantity:
100 μg

Research Discipline:
Epigenetics & Nuclear Function

Gene ID (Entrez):
NP_612373

Target Species:
Human

Form:
Purified

Applications:
Immunohistochemistry, Immunoprecipitation, Western Blot

Clone:
128-29-1

Conjugate:
Unconjugated

Gene:
PRMT9, PRMT10

Host Species:
Mouse

Purification Method:
Protein G Purified

Regulatory Status:
RUO

Primary or Secondary:
Primary

Test Specificity:
Clone detected GST fusion containing recombinant human PRMT9 fragment a.a. 401-550, as well as endogenous human, but not mouse, PRMT9 in cell lysates (Yang, Y., et al. (2015). Nat. Commun. 6:6428). Epitope region is present in both spliced isoforms of human PRMT9 reported by UniProt (Q6P2P2).

Content And Storage:
Stable for 1 year at 2°-8°C from date of receipt.

Isotype:
IgG1 κ

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MABE1119MI

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Antigen:
H2AK15ub

Classification:
Monoclonal

Concentration:
Please refer to lot specific datasheet.

Formulation:
__

Gene Accession No.:
__

Immunogen:
KLH-conjugated branched peptide corresponding to the ubiquitination site on human H2AK15.

Quantity:
100 μg

Research Discipline:
Epigenetics and Nuclear Function

Gene ID (Entrez):
NP_003500.1

Target Species:
Mouse, Drosophila, Human

Form:
Purified

Applications:
Western Blot, Immunocytochemistry

Clone:
EDL H2AK15-4

Conjugate:
Unconjugated

Gene:
__

Host Species:
Mouse

Purification Method:
Protein G Purified

Regulatory Status:
RUO

Primary or Secondary:
Primary

Test Specificity:
Clone EDL H2AK15-4 specifically recognizes histone H2A monoubiquitylated at Lys15, but not unmodified H2A. Does not recognize di-ubiquitin, K63 and K27 linked di-ubiquitin species.

Content And Storage:
Stable for 1 year at 2-8°C from date of receipt.

Isotype:
IgG2b κ

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Sigma Aldrich

MABE1120

clone N69, from mouse...


Antigen:
__

Classification:
__

Concentration:
__

Formulation:
__

Gene Accession No.:
__

Immunogen:
__

Quantity:
__

Research Discipline:
__

Gene ID (Entrez):
__

Target Species:
__

Form:
__

Applications:
__

Clone:
__

Conjugate:
__

Gene:
__

Host Species:
__

Purification Method:
__

Regulatory Status:
__

Primary or Secondary:
__

Test Specificity:
__

Content And Storage:
__

Isotype:
__

Img

MilliporeSigma™

MABE1120MI

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Antigen:
HSPA8/Hsc70

Classification:
Monoclonal

Concentration:
__

Formulation:
Purified mouse monoclonal IgG1κ antibody in buffer containing 0.1M Tris-Glycine (pH 7.4), 150mM NaCl with 0.05% sodium azide.

Gene Accession No.:
P19120

Immunogen:
Co-purified Hsc73 and Hsp72 proteins from bovine skeletal muscle.

Quantity:
100 μg

Research Discipline:
Epigenetics, Nuclear Function

Gene ID (Entrez):
NP_776770

Target Species:
Bovine, Human, Mouse, Rat

Form:
Purified

Applications:
Flow Cytometry, Immunocytochemistry, Immunohistochemistry, Western Blot

Clone:
N69

Conjugate:
Unconjugated

Gene:
__

Host Species:
Mouse

Purification Method:
Protein G purified

Regulatory Status:
RUO

Primary or Secondary:
Primary

Test Specificity:
Clone N69 reacts with the constitutively expressed HSPA8/Hsc70 (HSC73), but not the heat schock-inducible HSP72 (Demidov, O.N., et al. (1999). Eur. J. Cardiothorac. Surg. 16(4):444-449).

Content And Storage:
2°C to 8°C for one year from date of shipment

Isotype:
IgG1 κ