MABT823MI
MilliporeSigma™ alpha-Actin, Cardiac Muscle, Mouse, Unlabeled, Clone: 22D3,
Manufacturer: MilliporeSigma™
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | MABT823MI-Each-of-1 | In Stock | ₹ 38,734.72 |
MABT823MI - Each of 1
In Stock
Quantity
1
Base Price: ₹ 38,734.72
GST (18%): ₹ 6,972.25
Total Price: ₹ 45,706.97
Antigen
alpha-Actin, Cardiac Muscle
Classification
Monoclonal
Concentration
Please refer to lot specific datasheet.
Formulation
Purified mouse IgG1 in buffer containing 0.1M Tris-Glycine (pH 7.4), 150mM NaCl with 0.05% Sodium Azide.
Gene Accession No.
P68032
Immunogen
KLH-conjugated linear peptide corresponding to human mature alpha-cardiac actin N-terminal end sequence with an acetylated N-terminus.
Quantity
100 μL
Research Discipline
Cell Structure
Gene ID (Entrez)
NP_005150.1
Target Species
Goat
Form
Purified
Applications
Immunocytochemistry, Immunofluorescence, Western Blot
Clone
22D3
Conjugate
Unconjugated
Gene
ACTC1, ACTC, ASD5, CMD1R, CMH11, LVNC4
Host Species
Mouse
Purification Method
Protein G Purified
Regulatory Status
RUO
Primary or Secondary
Primary
Test Specificity
Clone 22D3 specifically detected alpha-cardiac actin in rat myocardium homogenates with no cross-reactivity toward - or -smooth muscle actin in rat aorta and chicken gizzard homogenates, cytoplasmic - or -actin in human platelet lysate, nor -skeletal actin in rat skeletal muscle homogenate (Driesen, R.B., et al. (2009). J. Cell. Mol. Med. 13(5):896-908).
Content And Storage
Stable for 1 year at 2°-8°C from date of receipt.
Isotype
IgG1 κ
Related Products
Description
- Actin, alpha cardiac muscle 1 (UniProt P68032; also known as Alpha-cardiac actin) is encoded by the ACTC1 (also known as ACTC, ASD5, CMD1R, CMH11, LVNC4) gene (Gene ID 70) in human
- Actins are globular multi-functional proteins that serve as the basic building blocks of cytoskeletal microfilaments and are among the most conserved eukaryotic proteins
- Six actin types exist, skeletal muscle alpha-actin is encoded by the ACTA1 gene, smooth muscle alpha-actin by the ACTA2 gene, cytoplasmic beta-actin by the ACTB gene, cardiac muscle alpha-actin by the ACTC1 gene, cytoplasmic gamma-actin by the ACTG1 gene, and smooth muscle gamma-actin by the ACTG2 (a.k.a
- ACTA3) gene
- Although actins show >90% overall sequence homology, isoforms do show spatial, temporal, and tissue-specific expresson patterns and only 50-60% homology is found in their 18 N-terminal residues
- Cytoplasmic - and -actins, are thought to be present in all cells, while the other four actin isoforms are typically found in specific adult muscle tissue types
- Actins exist in a variety of structural states, depending on the specific ionic conditions or the interaction with ligand proteins
- The oligomeric and polymeric forms that actin molecules assume are dependent on the distinct conformations they adopt.
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Antigen: alpha-Actin, Cardiac Muscle
Classification: Monoclonal
Concentration: Please refer to lot specific datasheet.
Formulation: Purified mouse IgG1 in buffer containing 0.1M Tris-Glycine (pH 7.4), 150mM NaCl with 0.05% Sodium Azide.
Gene Accession No.: P68032
Immunogen: KLH-conjugated linear peptide corresponding to human mature alpha-cardiac actin N-terminal end sequence with an acetylated N-terminus.
Quantity: 100 μL
Research Discipline: Cell Structure
Gene ID (Entrez): NP_005150.1
Target Species: Goat
Form: Purified
Applications: Immunocytochemistry, Immunofluorescence, Western Blot
Clone: 22D3
Conjugate: Unconjugated
Gene: ACTC1, ACTC, ASD5, CMD1R, CMH11, LVNC4
Host Species: Mouse
Purification Method: Protein G Purified
Regulatory Status: RUO
Primary or Secondary: Primary
Test Specificity: Clone 22D3 specifically detected alpha-cardiac actin in rat myocardium homogenates with no cross-reactivity toward - or -smooth muscle actin in rat aorta and chicken gizzard homogenates, cytoplasmic - or -actin in human platelet lysate, nor -skeletal actin in rat skeletal muscle homogenate (Driesen, R.B., et al. (2009). J. Cell. Mol. Med. 13(5):896-908).
Content And Storage: Stable for 1 year at 2°-8°C from date of receipt.
Isotype: IgG1 κ
Antigen:
alpha-Actin, Cardiac Muscle
Classification:
Monoclonal
Concentration:
Please refer to lot specific datasheet.
Formulation:
Purified mouse IgG1 in buffer containing 0.1M Tris-Glycine (pH 7.4), 150mM NaCl with 0.05% Sodium Azide.
Gene Accession No.:
P68032
Immunogen:
KLH-conjugated linear peptide corresponding to human mature alpha-cardiac actin N-terminal end sequence with an acetylated N-terminus.
Quantity:
100 μL
Research Discipline:
Cell Structure
Gene ID (Entrez):
NP_005150.1
Target Species:
Goat
Form:
Purified
Applications:
Immunocytochemistry, Immunofluorescence, Western Blot
Clone:
22D3
Conjugate:
Unconjugated
Gene:
ACTC1, ACTC, ASD5, CMD1R, CMH11, LVNC4
Host Species:
Mouse
Purification Method:
Protein G Purified
Regulatory Status:
RUO
Primary or Secondary:
Primary
Test Specificity:
Clone 22D3 specifically detected alpha-cardiac actin in rat myocardium homogenates with no cross-reactivity toward - or -smooth muscle actin in rat aorta and chicken gizzard homogenates, cytoplasmic - or -actin in human platelet lysate, nor -skeletal actin in rat skeletal muscle homogenate (Driesen, R.B., et al. (2009). J. Cell. Mol. Med. 13(5):896-908).
Content And Storage:
Stable for 1 year at 2°-8°C from date of receipt.
Isotype:
IgG1 κ
Antigen: beta-Actin
Classification: Monoclonal
Concentration: __
Formulation: Purified mouse monoclonal IgG1κ antibody in buffer containing 0.1M Tris-Glycine (pH 7.4), 150mM NaCl with 0.05% sodium azide.
Gene Accession No.: P60709
Immunogen: KLH-conjugated linear peptide corresponding to the the N-terminal sequence of human beta-Actin.
Quantity: 100 μg
Research Discipline: Cell Structure
Gene ID (Entrez): NP_001092.1
Target Species: Chicken, Human, Mouse, Rat
Form: Purified
Applications: Immunocytochemistry, Western Blot
Clone: 4C2
Conjugate: __
Gene: __
Host Species: Mouse
Purification Method: Protein G Purified
Regulatory Status: RUO
Primary or Secondary: Primary
Test Specificity: __
Content And Storage: Stable for one year at 2°C to 8°C from date of receipt.
Isotype: IgG1 κ
Antigen:
beta-Actin
Classification:
Monoclonal
Concentration:
__
Formulation:
Purified mouse monoclonal IgG1κ antibody in buffer containing 0.1M Tris-Glycine (pH 7.4), 150mM NaCl with 0.05% sodium azide.
Gene Accession No.:
P60709
Immunogen:
KLH-conjugated linear peptide corresponding to the the N-terminal sequence of human beta-Actin.
Quantity:
100 μg
Research Discipline:
Cell Structure
Gene ID (Entrez):
NP_001092.1
Target Species:
Chicken, Human, Mouse, Rat
Form:
Purified
Applications:
Immunocytochemistry, Western Blot
Clone:
4C2
Conjugate:
__
Gene:
__
Host Species:
Mouse
Purification Method:
Protein G Purified
Regulatory Status:
RUO
Primary or Secondary:
Primary
Test Specificity:
__
Content And Storage:
Stable for one year at 2°C to 8°C from date of receipt.
Isotype:
IgG1 κ
Antigen: Actin
Classification: Monoclonal
Concentration: __
Formulation: Purified mouse monoclonal IgMκ antibody in PBS with 0.05% sodium azide.
Gene Accession No.: P68135
Immunogen: Reconstituted complex of rabbit skeletal muscle actin and calf thymus profilin.
Quantity: 100 μL
Research Discipline: Cell Structure
Gene ID (Entrez): __
Target Species: Chicken, Frog, Human, Monkey, Mouse, Rabbit, Rat
Form: Purified
Applications: Immunocytochemistry, Western Blot
Clone: 2G2
Conjugate: Unconjugated
Gene: __
Host Species: Mouse
Purification Method: Purified by ion-exchange chromatography
Regulatory Status: RUO
Primary or Secondary: Primary
Test Specificity: Clone 2G2 recognizes a tripartite epitope identified in a region of the actin monomer (G-actin) that is not exposed on the surface the filamentous F-actin, but is accessible on the surface of profilin-complexed actin molecule (Gonsior, S.M., et al. (1999). J. Cell Sci. 112 (Pt 6):797-809).
Content And Storage: 2°C to 8°C for one year from date of shipment
Isotype: IgM κ
Antigen:
Actin
Classification:
Monoclonal
Concentration:
__
Formulation:
Purified mouse monoclonal IgMκ antibody in PBS with 0.05% sodium azide.
Gene Accession No.:
P68135
Immunogen:
Reconstituted complex of rabbit skeletal muscle actin and calf thymus profilin.
Quantity:
100 μL
Research Discipline:
Cell Structure
Gene ID (Entrez):
__
Target Species:
Chicken, Frog, Human, Monkey, Mouse, Rabbit, Rat
Form:
Purified
Applications:
Immunocytochemistry, Western Blot
Clone:
2G2
Conjugate:
Unconjugated
Gene:
__
Host Species:
Mouse
Purification Method:
Purified by ion-exchange chromatography
Regulatory Status:
RUO
Primary or Secondary:
Primary
Test Specificity:
Clone 2G2 recognizes a tripartite epitope identified in a region of the actin monomer (G-actin) that is not exposed on the surface the filamentous F-actin, but is accessible on the surface of profilin-complexed actin molecule (Gonsior, S.M., et al. (1999). J. Cell Sci. 112 (Pt 6):797-809).
Content And Storage:
2°C to 8°C for one year from date of shipment
Isotype:
IgM κ
Antigen: Dystrophin
Classification: Monoclonal
Concentration: Please refer to lot specific datasheet.
Formulation: Purified mouse IgG2a Alexa Fluor™ 488 conjugate in buffer containing PBS, 1.5% BSA with 0.05% Sodium Azide.
Gene Accession No.: P11532
Immunogen: TrpE-tagged recombinant protein corresponding to the Exon 43-coded pectrin-like repeat 16 region of human Dystrophin.
Quantity: 100 μL
Research Discipline: Cell Structure
Gene ID (Entrez): NP_000100
Target Species: Human
Form: __
Applications: Immunofluorescence
Clone: 2C6 (MANDYS106)
Conjugate: Alexa Fluor 488
Gene: DMD, BMD, CMD3B, DXS142, DXS164, DXS206, DXS230, DXS239, DXS268, DXS269, DXS270, DXS272, MRX85
Host Species: Mouse
Purification Method: Protein G Purified
Regulatory Status: RUO
Primary or Secondary: Primary
Test Specificity: Detects dystrophin spliced isoforms 1-4, but not isoforms 5-10, or utrophin. Positive muscle membrane staining of tissue samples from healthy donors, reduced staining of Becker muscular dystrophy (BMD) biopsies, and no staining is seen with Duchenne muscular dystrophy (DMD) biopsy samples (Anthony, K., et al. (2011). Brain. 134(Pt 12):3547-3559).
Content And Storage: Stable for 1 year at 2°-8°C from date of receipt.
Isotype: IgG2a κ
Antigen:
Dystrophin
Classification:
Monoclonal
Concentration:
Please refer to lot specific datasheet.
Formulation:
Purified mouse IgG2a Alexa Fluor™ 488 conjugate in buffer containing PBS, 1.5% BSA with 0.05% Sodium Azide.
Gene Accession No.:
P11532
Immunogen:
TrpE-tagged recombinant protein corresponding to the Exon 43-coded pectrin-like repeat 16 region of human Dystrophin.
Quantity:
100 μL
Research Discipline:
Cell Structure
Gene ID (Entrez):
NP_000100
Target Species:
Human
Form:
__
Applications:
Immunofluorescence
Clone:
2C6 (MANDYS106)
Conjugate:
Alexa Fluor 488
Gene:
DMD, BMD, CMD3B, DXS142, DXS164, DXS206, DXS230, DXS239, DXS268, DXS269, DXS270, DXS272, MRX85
Host Species:
Mouse
Purification Method:
Protein G Purified
Regulatory Status:
RUO
Primary or Secondary:
Primary
Test Specificity:
Detects dystrophin spliced isoforms 1-4, but not isoforms 5-10, or utrophin. Positive muscle membrane staining of tissue samples from healthy donors, reduced staining of Becker muscular dystrophy (BMD) biopsies, and no staining is seen with Duchenne muscular dystrophy (DMD) biopsy samples (Anthony, K., et al. (2011). Brain. 134(Pt 12):3547-3559).
Content And Storage:
Stable for 1 year at 2°-8°C from date of receipt.
Isotype:
IgG2a κ