MABN1780MI
MilliporeSigma™ Cereblon, Mouse, Unlabeled, Clone: 3B12F7,
Manufacturer: MilliporeSigma™
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | MABN1780MI-Each-of-1 | In Stock | ₹ 40,531.48 |
MABN1780MI - Each of 1
In Stock
Quantity
1
Base Price: ₹ 40,531.48
GST (18%): ₹ 7,295.666
Total Price: ₹ 47,827.146
Antigen
Cereblon
Classification
Monoclonal
Concentration
Please refer to lot specific datasheet.
Formulation
Purified mouse IgG1 in buffer containing 0.1M Tris-Glycine (pH 7.4), 150mM NaCl with 0.05% Sodium Azide.
Gene Accession No.
Q96SW2
Immunogen
KLH-conjugated linear peptide corresponding to a sequence near the N-terminus of human cereblon.
Quantity
100 μL
Research Discipline
Apoptosis & Cancer
Gene ID (Entrez)
NP_057386
Target Species
Human
Form
Purified
Applications
Western Blot
Clone
3B12F7
Conjugate
Unconjugated
Gene
CRBN, AD-006, MRT2A
Host Species
Mouse
Purification Method
Protein G Purified
Regulatory Status
RUO
Primary or Secondary
Primary
Test Specificity
Clone 3B12F7 was raised against an N-terminal sequence present in human cereblon spliced isoforms 1&2 and murine cereblon spliced isoforms 1&3 (UniProt Q96SW2 and Q8C7D2). Immunogen sequence is well conserved in essentially all mammalian species.
Content And Storage
Stable for 1 year at 2°-8°C from date of receipt.
Isotype
IgG1 κ
Related Products
Description
- Protein cereblon (UniProt Q96SW2) is encoded by the CRBN (also known as AD-006, MRT2A) gene (Gene ID 51185) in human
- Originally identified as a genetic cause of inherited autosomal recessive mental retardation, the human CRBN gene encodes a substrate receptor of a ubiquitin E3 cullin 4-RING ligase (CRL4) complex
- A cullin-4 (CULT4) protein provides a scaffold for assembling RING box-domain protein (RBX1) and the adaptor protein damaged DNA-binding protein 1 (DDB1) to form a CRL4 E3 complex, where RBX1 serves as the docking site for the activated E2 protein, and DDB1 recruits substrate-specificity receptors or DCAFs (DDB1-cullin 4-associated factors) to form the substrate-presenting side of the CRL4 complex
- Thalidomide and its analogs (lenalidomide, pomalidomide and apremilast) are immunomodulatory drugs (IMiDs) that exert their antiproliferative effects on multiple myeloma (MM) cells and immunomodulatory effects on T cells by directly binding cereblon and promoting recruitment of the hematopoietic zinc-finger transcription factors Ikaros (IKZF1) and Aiolos (IKZF3), leading to their ubiquitination and proteasomal degradation
- Human cereblon is a 442-amino acid protein composed of a Lon protease-like scaffold (a.a
- 80-317) with a helical insertion that mediates DDB1 binding and a thalidomide-binding domain (TBD; a.a
- 339-442).
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Antigen: Cereblon
Classification: Monoclonal
Concentration: Please refer to lot specific datasheet.
Formulation: Purified mouse IgG1 in buffer containing 0.1M Tris-Glycine (pH 7.4), 150mM NaCl with 0.05% Sodium Azide.
Gene Accession No.: Q96SW2
Immunogen: KLH-conjugated linear peptide corresponding to a sequence near the N-terminus of human cereblon.
Quantity: 100 μL
Research Discipline: Apoptosis & Cancer
Gene ID (Entrez): NP_057386
Target Species: Human
Form: Purified
Applications: Western Blot
Clone: 3B12F7
Conjugate: Unconjugated
Gene: CRBN, AD-006, MRT2A
Host Species: Mouse
Purification Method: Protein G Purified
Regulatory Status: RUO
Primary or Secondary: Primary
Test Specificity: Clone 3B12F7 was raised against an N-terminal sequence present in human cereblon spliced isoforms 1&2 and murine cereblon spliced isoforms 1&3 (UniProt Q96SW2 and Q8C7D2). Immunogen sequence is well conserved in essentially all mammalian species.
Content And Storage: Stable for 1 year at 2°-8°C from date of receipt.
Isotype: IgG1 κ
Antigen:
Cereblon
Classification:
Monoclonal
Concentration:
Please refer to lot specific datasheet.
Formulation:
Purified mouse IgG1 in buffer containing 0.1M Tris-Glycine (pH 7.4), 150mM NaCl with 0.05% Sodium Azide.
Gene Accession No.:
Q96SW2
Immunogen:
KLH-conjugated linear peptide corresponding to a sequence near the N-terminus of human cereblon.
Quantity:
100 μL
Research Discipline:
Apoptosis & Cancer
Gene ID (Entrez):
NP_057386
Target Species:
Human
Form:
Purified
Applications:
Western Blot
Clone:
3B12F7
Conjugate:
Unconjugated
Gene:
CRBN, AD-006, MRT2A
Host Species:
Mouse
Purification Method:
Protein G Purified
Regulatory Status:
RUO
Primary or Secondary:
Primary
Test Specificity:
Clone 3B12F7 was raised against an N-terminal sequence present in human cereblon spliced isoforms 1&2 and murine cereblon spliced isoforms 1&3 (UniProt Q96SW2 and Q8C7D2). Immunogen sequence is well conserved in essentially all mammalian species.
Content And Storage:
Stable for 1 year at 2°-8°C from date of receipt.
Isotype:
IgG1 κ
Antigen: __
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Antigen: HLA Class I Antigens
Classification: Monoclonal
Concentration: Please refer to lot specific datasheet.
Formulation: Purified mouse monoclonal antibody IgG2a in PBS without preservatives.
Gene Accession No.: P30443;Q96DW9;O19617;P13747
Immunogen: Membrane from human tonsil lymphocyte preparations (Barnstable, C.J., et al. (1978). Cell. 14(1):9-20).
Quantity: 100 μL
Research Discipline: Neuroscience
Gene ID (Entrez): NP_001229687;CAD30340;NP_001229971;NP_005507
Target Species: Human
Form: Purified
Applications: Affinity Purification, Flow Cytometry, Immunohistochemistry, Immunoprecipitation, Neutralization
Clone: W6/32
Conjugate: Unconjugated
Gene: HLA-A, HLAA, HLA-B, HLAB, HLA-C, HLAC, HLA-E, HLAE
Host Species: Mouse
Purification Method: Protein G Purified
Regulatory Status: RUO
Primary or Secondary: Primary
Test Specificity: Clone W6/32 targets HLA classe I heavy chains (HLA-A, -B, -C, -E, and possibly -F) in a light chain-dependent manner, although clone W6/32 does not exhibit affinity toward isolated light chain (β2-microglobulin or β2-m). A tight interaction between β2-m residue 89 and some residues within the heavy chain second domain might be responsible for the formation of the W6/32 antigenic determinant which, however, is only expressed (or exposed) when β2-m cannot covalently bind to the heavy chain Cys121 (Kahn-Perles, B., et al. (1987). J. Immunol. 138(7).2190-2196). Cell surface MHC I molecules reconstituted with allele-specific peptides and recombinant human β2-m that contains a methionine at the N-terminus is not recognized by W6/32 (Shields, M.J., and Ribaudo, R.K. (1998). Tissue Antigens. 51(5):567-570). Clone W6/32 is reported to bind murine, rat, rabbit and guinea pig HLA class I heavy chains only when they are associated with bovine β2-m, but not with their
Content And Storage: Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Isotype: IgG2a
Antigen:
HLA Class I Antigens
Classification:
Monoclonal
Concentration:
Please refer to lot specific datasheet.
Formulation:
Purified mouse monoclonal antibody IgG2a in PBS without preservatives.
Gene Accession No.:
P30443;Q96DW9;O19617;P13747
Immunogen:
Membrane from human tonsil lymphocyte preparations (Barnstable, C.J., et al. (1978). Cell. 14(1):9-20).
Quantity:
100 μL
Research Discipline:
Neuroscience
Gene ID (Entrez):
NP_001229687;CAD30340;NP_001229971;NP_005507
Target Species:
Human
Form:
Purified
Applications:
Affinity Purification, Flow Cytometry, Immunohistochemistry, Immunoprecipitation, Neutralization
Clone:
W6/32
Conjugate:
Unconjugated
Gene:
HLA-A, HLAA, HLA-B, HLAB, HLA-C, HLAC, HLA-E, HLAE
Host Species:
Mouse
Purification Method:
Protein G Purified
Regulatory Status:
RUO
Primary or Secondary:
Primary
Test Specificity:
Clone W6/32 targets HLA classe I heavy chains (HLA-A, -B, -C, -E, and possibly -F) in a light chain-dependent manner, although clone W6/32 does not exhibit affinity toward isolated light chain (β2-microglobulin or β2-m). A tight interaction between β2-m residue 89 and some residues within the heavy chain second domain might be responsible for the formation of the W6/32 antigenic determinant which, however, is only expressed (or exposed) when β2-m cannot covalently bind to the heavy chain Cys121 (Kahn-Perles, B., et al. (1987). J. Immunol. 138(7).2190-2196). Cell surface MHC I molecules reconstituted with allele-specific peptides and recombinant human β2-m that contains a methionine at the N-terminus is not recognized by W6/32 (Shields, M.J., and Ribaudo, R.K. (1998). Tissue Antigens. 51(5):567-570). Clone W6/32 is reported to bind murine, rat, rabbit and guinea pig HLA class I heavy chains only when they are associated with bovine β2-m, but not with their
Content And Storage:
Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Isotype:
IgG2a
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