MABT81925UL
Glycosaminoglycan, Mouse anti-Shark, Human, Chicken, Clone: PG-4 , Millipore Sigma™
Manufacturer: MilliporeSigma
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| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | MABT81925UL-Each-of-1 | In Stock | ₹ 16,456.61 |
MABT81925UL - Each of 1
In Stock
Quantity
1
Base Price: ₹ 16,456.61
GST (18%): ₹ 2,962.19
Total Price: ₹ 19,418.80
Antigen
Glycosaminoglycan
Classification
Monoclonal
Conjugate
Unconjugated
Formulation
Purified mouse monoclonal antibody IgM in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Host Species
Mouse
Purification Method
Protein G purified
Regulatory Status
RUO
Primary or Secondary
Primary
Target Species
Shark, Human, Chicken
Form
Purified
Applications
ELISA, Immunohistochemistry (Paraffin), Western Blot
Clone
PG-4
Dilution
Western Blotting Analysis: A representative lot detected Glycosaminoglycan in Western Blotting applications (Sorrell, J.M., et. al. (1999). Histochem J. 31(8):549-58).ELISA Analysis: A representative lot detected Glycosaminoglycan in ELISA applications (Sorrell, J.M., et. al. (1999). Histochem J. 31(8):549-58).Immunohistochemistry Analysis: A representative lot detected Glycosaminoglycan in Immunohistochemistry applications (Sorrell, J.M., et. al. (1999). Histochem J. 31(8):549-58).
Gene Alias
Proteoglycan;Dermatan sulfate;Chondroitin sulfate
Immunogen
Crude membrane fractions from adult human dermal fibroblasts from breast skin.
Quantity
25 μL
Research Discipline
Cell Structure
Test Specificity
Clone PG4 detects glycosaminoglycan in skin cells.
Content And Storage
Stable for 1 year at 2-8°C from date of receipt.
Isotype
IgM κ
Description
- Anti-Glycosaminoglycan, skin specific, clone PG-4, Cat
- No
- MABT819, is a mouse monoclonal antibody that detects glycosaminoglycan and has been tested for use in ELISA, Immunohistochemistry (Paraffin), and Western Blotting
- Proteoglycans (PG), present in all tissues, are one of the best-studied classes of matrix molecules
- Over 50 members of PG have been characterized and studied for their biological significance
- PG consist of a protein core to which glycosaminoglycan chains are covalently linked
- Glycosaminoglycans (GAGs) are natural heteropolysaccharides that are present in every mammalian tissue
- They are composed of repeating disaccharide units that consist of either sulfated or non-sulfated monosaccharides
- Their molecular size and the sulfation type vary based on tissue type
- The major types of GAGs found in mammalian tissues are: hyaluronan, chondroitin sulfate, and dermatan sulfate, heparin and heparan sulfate, and keratan sulfate
- GAG chains are covalently bound to serine residues of the PG protein core via a tetrasaccharide linkage, consisting of xylose, two galactose residues and glucuronic acid
- This antibody, clone PG-4, recognizes antigen that is associated with extracellular matrices and surfaces of cultured human dermal fibroblasts and the surfaces of cultured human adult keratinocytes
- This antibody recognize native epitopes located within glycosaminoglycan chains and is detects both chondroitin sulfate (Versican) and dermatan sulfate (Decorin and Biglycan) proteoglycans
- Treatment of PG with chondroitinases is reported to abolish its immunoreactivity for all antigens
- (Ref.: Afratis, N et al
- (2012)
- FEBS Journal 279 (7); 1177-1197; Sorrell, JM et al
- (1999)
- Histochem
- J
- 31 (8); 549-558).
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Antigen: Glycosaminoglycan
Classification: Monoclonal
Conjugate: Unconjugated
Formulation: Purified mouse monoclonal antibody IgM in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Host Species: Mouse
Purification Method: Protein G purified
Regulatory Status: RUO
Primary or Secondary: Primary
Target Species: Shark, Human, Chicken
Form: Purified
Applications: ELISA, Immunohistochemistry (Paraffin), Western Blot
Clone: PG-4
Dilution: Western Blotting Analysis: A representative lot detected Glycosaminoglycan in Western Blotting applications (Sorrell, J.M., et. al. (1999). Histochem J. 31(8):549-58).ELISA Analysis: A representative lot detected Glycosaminoglycan in ELISA applications (Sorrell, J.M., et. al. (1999). Histochem J. 31(8):549-58).Immunohistochemistry Analysis: A representative lot detected Glycosaminoglycan in Immunohistochemistry applications (Sorrell, J.M., et. al. (1999). Histochem J. 31(8):549-58).
Gene Alias: Proteoglycan;Dermatan sulfate;Chondroitin sulfate
Immunogen: Crude membrane fractions from adult human dermal fibroblasts from breast skin.
Quantity: 25 μL
Research Discipline: Cell Structure
Test Specificity: Clone PG4 detects glycosaminoglycan in skin cells.
Content And Storage: Stable for 1 year at 2-8°C from date of receipt.
Isotype: IgM κ
Antigen:
Glycosaminoglycan
Classification:
Monoclonal
Conjugate:
Unconjugated
Formulation:
Purified mouse monoclonal antibody IgM in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Host Species:
Mouse
Purification Method:
Protein G purified
Regulatory Status:
RUO
Primary or Secondary:
Primary
Target Species:
Shark, Human, Chicken
Form:
Purified
Applications:
ELISA, Immunohistochemistry (Paraffin), Western Blot
Clone:
PG-4
Dilution:
Western Blotting Analysis: A representative lot detected Glycosaminoglycan in Western Blotting applications (Sorrell, J.M., et. al. (1999). Histochem J. 31(8):549-58).ELISA Analysis: A representative lot detected Glycosaminoglycan in ELISA applications (Sorrell, J.M., et. al. (1999). Histochem J. 31(8):549-58).Immunohistochemistry Analysis: A representative lot detected Glycosaminoglycan in Immunohistochemistry applications (Sorrell, J.M., et. al. (1999). Histochem J. 31(8):549-58).
Gene Alias:
Proteoglycan;Dermatan sulfate;Chondroitin sulfate
Immunogen:
Crude membrane fractions from adult human dermal fibroblasts from breast skin.
Quantity:
25 μL
Research Discipline:
Cell Structure
Test Specificity:
Clone PG4 detects glycosaminoglycan in skin cells.
Content And Storage:
Stable for 1 year at 2-8°C from date of receipt.
Isotype:
IgM κ
Antigen: Integrin beta1 (CD29)
Classification: Monoclonal
Conjugate: Unconjugated
Formulation: Purified rat monoclonal antibody IgG2a in PBS without preservatives.
Host Species: Rat
Purification Method: Protein G purified
Regulatory Status: RUO
Primary or Secondary: Primary
Target Species: Human
Form: Purified
Applications: Immunocytochemistry, Immunoprecipitation, Inhibition Assays
Clone: mAb13
Dilution: Inhibition Analysis: A representative lot blocked cell attachment in Inhibition studies (Akiyama, S.K., et. al. (1989). J Cell Biol. 109(2):863-75; Humphries, J.D., et. al. (2005). J Biol Chem. 280(11):10234-43). Immunocytochemistry Analysis: A representative lot detected Integrin beta1 (CD29) in Immunocytochemistry applications (Akiyama, S.K., et. al. (1989). J Cell Biol. 109(2):863-75; Humphries, J.D., et. al. (2005). J Biol Chem. 280(11):10234-43). Immunoprecipitation Analysis: A representative lot detected Integrin beta1 (CD29) in Immunoprecipitation applications (Akiyama, S.K., et. al. (1989). J Cell Biol. 109(2):863-75). Inhibition Analysis: A representative lot blocked HT-1080 cells adhesion to fibronectin coated plates (Courtesy of Dr. Kenneth M. Yamada, M.D., Ph.D., NIDCR, National Institutes of Health, Bethesda, MD USA).
Gene Alias: Fibronectin receptor subunit beta;Glycoprotein IIa;GPIIA;VLA-4 subunit beta;CD29
Immunogen: Fibronectin receptor (alpha V beta 1) purified from human placenta.
Quantity: 25 μL
Research Discipline: Cell Structure
Test Specificity: Clone mAb13 specifically detects beta 1 subunit of human integrin. It targets an epitope within 12 amino acids from the N-terminal region.
Content And Storage: Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Isotype: IgG2a κ
Antigen:
Integrin beta1 (CD29)
Classification:
Monoclonal
Conjugate:
Unconjugated
Formulation:
Purified rat monoclonal antibody IgG2a in PBS without preservatives.
Host Species:
Rat
Purification Method:
Protein G purified
Regulatory Status:
RUO
Primary or Secondary:
Primary
Target Species:
Human
Form:
Purified
Applications:
Immunocytochemistry, Immunoprecipitation, Inhibition Assays
Clone:
mAb13
Dilution:
Inhibition Analysis: A representative lot blocked cell attachment in Inhibition studies (Akiyama, S.K., et. al. (1989). J Cell Biol. 109(2):863-75; Humphries, J.D., et. al. (2005). J Biol Chem. 280(11):10234-43). Immunocytochemistry Analysis: A representative lot detected Integrin beta1 (CD29) in Immunocytochemistry applications (Akiyama, S.K., et. al. (1989). J Cell Biol. 109(2):863-75; Humphries, J.D., et. al. (2005). J Biol Chem. 280(11):10234-43). Immunoprecipitation Analysis: A representative lot detected Integrin beta1 (CD29) in Immunoprecipitation applications (Akiyama, S.K., et. al. (1989). J Cell Biol. 109(2):863-75). Inhibition Analysis: A representative lot blocked HT-1080 cells adhesion to fibronectin coated plates (Courtesy of Dr. Kenneth M. Yamada, M.D., Ph.D., NIDCR, National Institutes of Health, Bethesda, MD USA).
Gene Alias:
Fibronectin receptor subunit beta;Glycoprotein IIa;GPIIA;VLA-4 subunit beta;CD29
Immunogen:
Fibronectin receptor (alpha V beta 1) purified from human placenta.
Quantity:
25 μL
Research Discipline:
Cell Structure
Test Specificity:
Clone mAb13 specifically detects beta 1 subunit of human integrin. It targets an epitope within 12 amino acids from the N-terminal region.
Content And Storage:
Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Isotype:
IgG2a κ
Antigen: __
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Antigen: alpha-Actin, Cardiac Muscle
Classification: Monoclonal
Conjugate: Unconjugated
Formulation: Purified mouse IgG1 in buffer containing 0.1M Tris-Glycine (pH 7.4), 150mM NaCl with 0.05% Sodium Azide.
Host Species: Mouse
Purification Method: Protein G Purified
Regulatory Status: RUO
Primary or Secondary: Primary
Target Species: Goat
Form: Purified
Applications: Immunocytochemistry, Immunofluorescence, Western Blot
Clone: 22D3
Dilution: __
Gene Alias: __
Immunogen: KLH-conjugated linear peptide corresponding to human mature alpha-cardiac actin N-terminal end sequence with an acetylated N-terminus.
Quantity: 100 μL
Research Discipline: Cell Structure
Test Specificity: Clone 22D3 specifically detected alpha-cardiac actin in rat myocardium homogenates with no cross-reactivity toward - or -smooth muscle actin in rat aorta and chicken gizzard homogenates, cytoplasmic - or -actin in human platelet lysate, nor -skeletal actin in rat skeletal muscle homogenate (Driesen, R.B., et al. (2009). J. Cell. Mol. Med. 13(5):896-908).
Content And Storage: Stable for 1 year at 2°-8°C from date of receipt.
Isotype: IgG1 κ
Antigen:
alpha-Actin, Cardiac Muscle
Classification:
Monoclonal
Conjugate:
Unconjugated
Formulation:
Purified mouse IgG1 in buffer containing 0.1M Tris-Glycine (pH 7.4), 150mM NaCl with 0.05% Sodium Azide.
Host Species:
Mouse
Purification Method:
Protein G Purified
Regulatory Status:
RUO
Primary or Secondary:
Primary
Target Species:
Goat
Form:
Purified
Applications:
Immunocytochemistry, Immunofluorescence, Western Blot
Clone:
22D3
Dilution:
__
Gene Alias:
__
Immunogen:
KLH-conjugated linear peptide corresponding to human mature alpha-cardiac actin N-terminal end sequence with an acetylated N-terminus.
Quantity:
100 μL
Research Discipline:
Cell Structure
Test Specificity:
Clone 22D3 specifically detected alpha-cardiac actin in rat myocardium homogenates with no cross-reactivity toward - or -smooth muscle actin in rat aorta and chicken gizzard homogenates, cytoplasmic - or -actin in human platelet lysate, nor -skeletal actin in rat skeletal muscle homogenate (Driesen, R.B., et al. (2009). J. Cell. Mol. Med. 13(5):896-908).
Content And Storage:
Stable for 1 year at 2°-8°C from date of receipt.
Isotype:
IgG1 κ